Leucobacter komagatae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales;

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2703 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D0HVG9|A0A0D0HVG9_9MICO Peptidyl-prolyl cis-trans isomerase OS=Leucobacter komagatae OX=55969 GN=SD72_14515 PE=3 SV=1
II1 pKa = 6.54ITTNGTEE8 pKa = 4.1PQNPLITYY16 pKa = 7.69ATTEE20 pKa = 3.98VGGGKK25 pKa = 9.38ILTAMYY31 pKa = 10.3AGLVSFTASGEE42 pKa = 4.1IEE44 pKa = 4.24NEE46 pKa = 3.91VAEE49 pKa = 4.82SIEE52 pKa = 4.32SDD54 pKa = 4.67DD55 pKa = 3.74AALWTITIEE64 pKa = 4.73DD65 pKa = 3.69GWTFSDD71 pKa = 4.59GEE73 pKa = 4.68AITAQTFVDD82 pKa = 3.66TWTNAAVDD90 pKa = 3.55PAGAYY95 pKa = 8.36WFGNIEE101 pKa = 4.13GTTEE105 pKa = 4.01EE106 pKa = 5.38GDD108 pKa = 3.71TAPTGLTVVDD118 pKa = 4.91DD119 pKa = 3.98LTFTVQLKK127 pKa = 7.83TAEE130 pKa = 4.07ADD132 pKa = 3.65FPLRR136 pKa = 11.84LAYY139 pKa = 8.24TAYY142 pKa = 10.08MPLPSAAFDD151 pKa = 3.62DD152 pKa = 4.25MEE154 pKa = 5.75AFGEE158 pKa = 4.12NPIGNGPYY166 pKa = 9.65MLDD169 pKa = 3.78GEE171 pKa = 4.86GAWRR175 pKa = 11.84HH176 pKa = 5.09EE177 pKa = 4.5EE178 pKa = 4.2GVSLVTNPTYY188 pKa = 10.75DD189 pKa = 3.64GNRR192 pKa = 11.84KK193 pKa = 8.15PVNGGLEE200 pKa = 3.93FVFYY204 pKa = 11.15NDD206 pKa = 4.03LASAYY211 pKa = 10.58ADD213 pKa = 3.48AQSGNLDD220 pKa = 3.66ILDD223 pKa = 4.43SVPDD227 pKa = 3.95TAFGTYY233 pKa = 7.48QTDD236 pKa = 3.75FPDD239 pKa = 3.59RR240 pKa = 11.84FVNEE244 pKa = 3.89PAAIFQGFNIPYY256 pKa = 8.54YY257 pKa = 10.21IPNFEE262 pKa = 5.08DD263 pKa = 4.14GEE265 pKa = 4.28EE266 pKa = 4.32GKK268 pKa = 10.44LRR270 pKa = 11.84RR271 pKa = 11.84AAISMSIDD279 pKa = 3.02RR280 pKa = 11.84AEE282 pKa = 3.76ITEE285 pKa = 4.56TIFQEE290 pKa = 4.23TRR292 pKa = 11.84TPATDD297 pKa = 3.47FTSPVLPGWTDD308 pKa = 3.22SLAGAEE314 pKa = 4.1VLDD317 pKa = 4.35YY318 pKa = 11.29NPEE321 pKa = 3.76EE322 pKa = 4.17AVRR325 pKa = 11.84LWEE328 pKa = 4.22QADD331 pKa = 3.37ALAPYY336 pKa = 9.99EE337 pKa = 4.37GTFTLAYY344 pKa = 9.87NADD347 pKa = 4.01GGHH350 pKa = 5.32QAWVDD355 pKa = 3.32AVTNSISNTLGIAAEE370 pKa = 4.32GLSLPTFAAALDD382 pKa = 3.85QRR384 pKa = 11.84SSGTLTGATRR394 pKa = 11.84SGWQGDD400 pKa = 4.21FPTQANFLSPLYY412 pKa = 9.79STGGSSNYY420 pKa = 9.81EE421 pKa = 4.36GYY423 pKa = 10.88SSAAFDD429 pKa = 3.84AKK431 pKa = 10.67LGEE434 pKa = 4.19AAAASSIEE442 pKa = 3.94EE443 pKa = 4.47SIDD446 pKa = 3.59LYY448 pKa = 11.39HH449 pKa = 6.87EE450 pKa = 4.22AQEE453 pKa = 4.47ILLEE457 pKa = 4.47DD458 pKa = 4.52LPAIPLWYY466 pKa = 10.3SNAVGVWGDD475 pKa = 3.47DD476 pKa = 3.51LEE478 pKa = 4.54NVVFGWDD485 pKa = 3.67SVPLHH490 pKa = 5.72HH491 pKa = 7.11QITKK495 pKa = 10.22GG496 pKa = 3.28

Molecular weight: 53.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D0HUF5|A0A0D0HUF5_9MICO DNA gyrase subunit B OS=Leucobacter komagatae OX=55969 GN=gyrB PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.23VHH17 pKa = 5.46GFRR20 pKa = 11.84SRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIITARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.68GRR40 pKa = 11.84TKK42 pKa = 10.85LSAA45 pKa = 3.61

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2703

0

2703

881507

29

3160

326.1

34.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.42 ± 0.057

0.495 ± 0.011

5.624 ± 0.044

6.247 ± 0.042

3.263 ± 0.031

9.103 ± 0.043

1.897 ± 0.021

4.758 ± 0.032

2.29 ± 0.035

10.218 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.872 ± 0.019

2.232 ± 0.027

5.154 ± 0.028

2.948 ± 0.024

6.661 ± 0.054

5.878 ± 0.027

6.118 ± 0.045

8.553 ± 0.045

1.353 ± 0.02

1.916 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski