Paraburkholderia caballeronis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6189 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H7UIR0|A0A1H7UIR0_9BURK Probable 2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase OS=Paraburkholderia caballeronis OX=416943 GN=mdcB PE=3 SV=1
MM1 pKa = 7.61ALASAGKK8 pKa = 10.05CLLSYY13 pKa = 10.85RR14 pKa = 11.84LDD16 pKa = 3.37EE17 pKa = 4.46SRR19 pKa = 11.84QSRR22 pKa = 11.84SCIDD26 pKa = 3.82SNPSTQEE33 pKa = 3.29EE34 pKa = 4.54SDD36 pKa = 3.77AVHH39 pKa = 6.25CRR41 pKa = 11.84YY42 pKa = 9.48PVDD45 pKa = 3.78HH46 pKa = 7.28KK47 pKa = 11.47SPATGRR53 pKa = 11.84PFSFSCRR60 pKa = 11.84LLVATTAAFPFMLAAGPAYY79 pKa = 10.24ADD81 pKa = 3.69GAGGPNSAALGGSSGINGGAGGGGGVGGVGGANTGANVGAGGNGSATGTGGAGSSGTATGGGSPGGGGSGGAAGTAANPAGVGGGAGGNASCGLCVAGLGGGGGGGGGDD190 pKa = 3.54GFVGATLNGVTGVMTGGAGGNGGNATSNSLLAGADD225 pKa = 3.54GGSGGGGGAGAVLSGSGSTSTAANVSGGAGGSGGTALSSGGAGFGGGGGSGLVDD279 pKa = 3.06SGVNVTNSFSITGGNGGAGGSGAQAGTGRR308 pKa = 11.84SGGNGGAGVTGSSFTLTNTGIVQGGNGGATGGNGSGTGTGVLGAGGVGVQGANLQIVNGGTISGGTAGDD377 pKa = 3.35SSTRR381 pKa = 11.84ANAIAFTGGANSLQLQLGWSFVGNVVSTGGTSTLILGGDD420 pKa = 3.78ATNVSANQAGTSVSTVFALSQIGTVFQGFSGFQKK454 pKa = 9.6TGASTWQLIGSTSDD468 pKa = 3.24STPWTISAGVLQLGNGTTNGSITGNVTNNATLAFDD503 pKa = 4.2PASGTTLTNASEE515 pKa = 4.18ISGTGQVQQIGAGITVLSGVNSYY538 pKa = 11.53AGGTAINAGTLQVAADD554 pKa = 4.06ANLGAAAGTLAFNGGTLNTTASFASSRR581 pKa = 11.84ATTLDD586 pKa = 3.12AGGGTFDD593 pKa = 3.92VGSGTTLTMGGAISGAGLLTKK614 pKa = 9.96TDD616 pKa = 3.43TGSLVLTGTNSYY628 pKa = 11.31AGGTTISAGTLQLGDD643 pKa = 3.48GTTNGSITGNVTNNATLAFDD663 pKa = 4.36PAASTTLTNTSAISGTGQAQQIGSGTTVLTGASTYY698 pKa = 10.47TGGTTISAGVLQLGDD713 pKa = 3.44GTTNGSITGNVTNDD727 pKa = 2.54ATFAFDD733 pKa = 3.8PASGTTMTNPGAISGTGQVQQIGAGTTVLTGVNTYY768 pKa = 9.72TGGTTISGGVLQLGDD783 pKa = 3.57GTTNGSITGSVTNNATLAFDD803 pKa = 4.2PASGTTITNAGVISGSGDD821 pKa = 3.35VQQIGAGITMLSGANTYY838 pKa = 9.63TGGTTIDD845 pKa = 3.38AGTLQVAADD854 pKa = 4.3DD855 pKa = 4.34NLGGAAGALTFNGGTLNTTASFASSRR881 pKa = 11.84ATTLDD886 pKa = 3.12AGGGTFDD893 pKa = 3.92VGSGTTLTMGGTISGAGLLTKK914 pKa = 10.0TDD916 pKa = 3.29TGVLVLTGTNGYY928 pKa = 7.98TGGTTISAGTLQLGNGTTNGSITGNVTNNATLAFDD963 pKa = 4.36PAASTTLTNASAISGTGQVQQIGIGTTVLTGASSYY998 pKa = 10.87TGGTTISAGVLQLGDD1013 pKa = 3.44GTTNGSITGNVTNDD1027 pKa = 2.64ATLAFDD1033 pKa = 4.24PATGTTMTNSGLISGAGQVQQIGAGTTVLSGTNAYY1068 pKa = 8.91TGGTTIDD1075 pKa = 3.58AGTLQLANASAAGTGTVTVDD1095 pKa = 3.36TAAGNTAQGLSLAFSSAGNFANTLSGSGVTTVAAGSALATITGSNASYY1143 pKa = 10.66AGNWQIAGNAAVAPASTTSTANLGTGTVNVAAGGGLTVATSGAFAFNNALTGSGALSASSGGQAFSFGSAAGSAFQGTATLSNDD1227 pKa = 3.03TFALAGANTTALTNATLVLGAGNVTTVGDD1256 pKa = 4.0GNHH1259 pKa = 7.05TIGALTFDD1267 pKa = 3.93GGTAVFDD1274 pKa = 3.82GTAPAQQVATSLVTTGALDD1293 pKa = 3.27ISGSGAVRR1301 pKa = 11.84INVPNPYY1308 pKa = 9.23EE1309 pKa = 4.29PPVPTPSGDD1318 pKa = 3.26LSLFQQSQMNNGLKK1332 pKa = 10.45LIAAASVTGTGSGLALEE1349 pKa = 4.84DD1350 pKa = 3.53QNGNAVSAAQNVNVQQNGNVVAIGTYY1376 pKa = 10.16DD1377 pKa = 3.48YY1378 pKa = 11.84ALGTGASDD1386 pKa = 3.51DD1387 pKa = 3.84GLYY1390 pKa = 10.58VGYY1393 pKa = 10.18QLNQLALQAGQSLTLTEE1410 pKa = 4.98DD1411 pKa = 3.38PGATGADD1418 pKa = 3.35ADD1420 pKa = 4.97LAAQLTGTGNLVIAANDD1437 pKa = 3.55VVSLSNAGNTFTGTTLVQSGTLQANAANVIASSGAVDD1474 pKa = 3.65VEE1476 pKa = 4.8STFDD1480 pKa = 3.44TNGFSQSLNNLTGTAAASIALSGNDD1505 pKa = 3.43TLTLNGGASPNTFAGVIGGAGSLVQASGTEE1535 pKa = 4.13VLTGANTYY1543 pKa = 9.63TGGTTISGGTLQLGDD1558 pKa = 3.6GTTNGSITGNVTNDD1572 pKa = 2.64ATLAFDD1578 pKa = 4.14PAAATTMTNPGLISGSGQVQQMGAGTTVFTGTQTYY1613 pKa = 9.31TGGTTISAGVLQLGDD1628 pKa = 3.57GTTNGAIAGSVTDD1641 pKa = 3.77NATLAFDD1648 pKa = 4.31PATGTTTSHH1657 pKa = 6.65AGVISGLGVVQQIGAGTTVLTGANTYY1683 pKa = 9.63TGGTTISSGVLQLGDD1698 pKa = 3.41GTTNGSIAGSVTNDD1712 pKa = 2.59ATLAFDD1718 pKa = 4.26PAAGTTTTNAGMISGSGDD1736 pKa = 3.31VQQIGAGITVLSGANAYY1753 pKa = 8.8TGGTTLNAGTLQVAADD1769 pKa = 4.3DD1770 pKa = 4.34NLGGAAGALTFNGGTLNTTASFASSRR1796 pKa = 11.84ATTLNAGGGTFGVGSATTLTMGGVISGAGALTKK1829 pKa = 10.1TDD1831 pKa = 3.37AGSLVLTGTNSYY1843 pKa = 9.58TGGTTISVGTLQLGNGTTNGSVAGNVTNNATLAFDD1878 pKa = 4.29PAAGTTMANASVIAGAGQVQQIGTGTTVLTGASSYY1913 pKa = 10.87TGGTTISAGVLQLGDD1928 pKa = 3.57GTTNGAITGNVTDD1941 pKa = 4.06NATLAFDD1948 pKa = 4.4PAASTTLTSAVAISGAGQVQQIGTGTTNLTGDD1980 pKa = 3.24SSTFAGATSVTSGTLLVSGALGDD2003 pKa = 4.14AGSTASVSSGGTLGGGGTLGGSVSVADD2030 pKa = 3.88GTLAPGTTSVSGSPGTLTIGGDD2052 pKa = 3.61LALTGASTLNYY2063 pKa = 10.17HH2064 pKa = 6.9FGQANVVGGPFNDD2077 pKa = 3.5LTEE2080 pKa = 4.31VGGNLTLAGTLNVTSSVGSFDD2101 pKa = 3.39PGIYY2105 pKa = 9.83RR2106 pKa = 11.84IISYY2110 pKa = 10.7GGALTDD2116 pKa = 3.43NGLTVGALPSGTSATVQTSIATEE2139 pKa = 4.06VNLLNTTGVTANFWDD2154 pKa = 4.99GASSPRR2160 pKa = 11.84NNGAVDD2166 pKa = 3.49GGDD2169 pKa = 3.98GVWQSAAGNDD2179 pKa = 2.99NWVASDD2185 pKa = 3.59GALNVPYY2192 pKa = 10.11TSGAFAIFAATPGTVTVDD2210 pKa = 3.19NSAGPVVSGGMQFAIGGYY2228 pKa = 8.42RR2229 pKa = 11.84VEE2231 pKa = 4.82GGPITMAAGGHH2242 pKa = 6.53FIRR2245 pKa = 11.84VGDD2248 pKa = 3.83GTEE2251 pKa = 4.36DD2252 pKa = 3.21GTGYY2256 pKa = 10.78VATIASALTGSGGIDD2271 pKa = 3.43KK2272 pKa = 10.77DD2273 pKa = 4.26DD2274 pKa = 4.84LGTLILTGANSYY2286 pKa = 9.5TGGTTISGGVLQLGDD2301 pKa = 3.57GTTNGSITGNVTNNATLAFDD2321 pKa = 4.24PASGTTMTNPGLISGTGQVQQIGAGTTVFTGTQTYY2356 pKa = 9.35TGGTTIGAGVLQLGDD2371 pKa = 3.51GTTNGALAGNVTNDD2385 pKa = 2.8ATLAFDD2391 pKa = 4.39PAASTTTTNSSVISGTGQVQQIGAGVTVLGGANAYY2426 pKa = 9.0TGGTAINAGTLQVAADD2442 pKa = 3.98NNLGGAAGALTFNGGALNTTASFASSRR2469 pKa = 11.84ATTLNANGGTFDD2481 pKa = 3.62VDD2483 pKa = 3.19AATTLTMSGALSGAGGLTKK2502 pKa = 10.19TDD2504 pKa = 3.02TGTLVLTGTNTYY2516 pKa = 11.07AGGTTISGGTLQLGDD2531 pKa = 3.57GTTNGVITGNVTNNATLAFDD2551 pKa = 4.26PAAGTTLTSAVAISGAGLVQQTGVGTTNLTGDD2583 pKa = 3.34SSAFSGVTNVTGGALLVSGALGGAGSTTTASSGGTLGGGGTLGGNVSVADD2633 pKa = 3.88GTLAPGDD2640 pKa = 3.72ARR2642 pKa = 11.84LGGGPGTLTIGGDD2655 pKa = 3.61LALTGASTLDD2665 pKa = 3.46YY2666 pKa = 11.11HH2667 pKa = 7.19FGQANVIGGPFNDD2680 pKa = 3.89LTDD2683 pKa = 3.41VGGNLTLAGTLNVTSSVGSFDD2704 pKa = 3.39PGIYY2708 pKa = 9.79RR2709 pKa = 11.84IIGYY2713 pKa = 10.06GGGLTNNGLSIGALPAGTTAAVQTSIANEE2742 pKa = 3.95VNLLNTTGITADD2754 pKa = 3.44FWDD2757 pKa = 5.09GASSPRR2763 pKa = 11.84NNGAVDD2769 pKa = 3.49GGDD2772 pKa = 3.98GVWQSAAGNDD2782 pKa = 2.99NWVASDD2788 pKa = 3.59GALNVPYY2795 pKa = 10.28TNGAFAIFAAAPGTVTVDD2813 pKa = 3.17TSVGPVVSGGMQFASDD2829 pKa = 4.39GYY2831 pKa = 9.75RR2832 pKa = 11.84IQGGPITLAAGTDD2845 pKa = 4.51LIRR2848 pKa = 11.84VGDD2851 pKa = 3.99GTEE2854 pKa = 4.22DD2855 pKa = 3.45GAGYY2859 pKa = 10.27VATIASEE2866 pKa = 4.19LTGSGGIDD2874 pKa = 3.43KK2875 pKa = 10.72DD2876 pKa = 4.02DD2877 pKa = 4.88LGTLVLSGANTYY2889 pKa = 9.61TGGTTVSAGTLRR2901 pKa = 11.84LAGGGTLGAAGGATTVSGGLLDD2923 pKa = 5.62LGGTTQTQDD2932 pKa = 2.71AGLTLVSGEE2941 pKa = 4.36VNNGTLFSSTGFALQSGSASASLAGSGALDD2971 pKa = 3.42KK2972 pKa = 8.91TTAGTVTLTGANTYY2986 pKa = 9.38TGATTIDD2993 pKa = 3.74AGTLAIGSGGSIAASSGVTLTAAGAVFDD3021 pKa = 4.11VTAGGNQTVKK3031 pKa = 10.87DD3032 pKa = 3.84LAGVAGSTVDD3042 pKa = 3.74VGASTLNFGTGNSTTFAGSFTGSGALVWQGGGTFSLTGDD3081 pKa = 3.41SSRR3084 pKa = 11.84FGGTTTVAAGTFAIGTDD3101 pKa = 3.57AAPDD3105 pKa = 3.47ASLGGDD3111 pKa = 3.5VVVSGGTLKK3120 pKa = 11.05GFGTIGGNLANRR3132 pKa = 11.84SGTVAPGGSIGTLTVSGNYY3151 pKa = 6.69TQNAGGTLAIEE3162 pKa = 4.39VNPASASQLKK3172 pKa = 10.6VGGAASLDD3180 pKa = 3.55GKK3182 pKa = 10.5LALLFAPGTYY3192 pKa = 10.58NPTSYY3197 pKa = 11.42KK3198 pKa = 10.63LLTAQSVGGTFSAVSGSNPGGLTQSIDD3225 pKa = 3.45YY3226 pKa = 10.44HH3227 pKa = 6.2PADD3230 pKa = 3.24VTLRR3234 pKa = 11.84LSTPSPSSPSLPPPPLVVAPDD3255 pKa = 3.32NDD3257 pKa = 4.41TIYY3260 pKa = 10.31TALTSIAVLNAQQINGILLDD3280 pKa = 4.34RR3281 pKa = 11.84IGQRR3285 pKa = 11.84RR3286 pKa = 11.84AGIADD3291 pKa = 3.75GPVAGLGDD3299 pKa = 4.3PAPQLQYY3306 pKa = 11.53ASAFGPLPAVQSLPTAARR3324 pKa = 11.84GAWFRR3329 pKa = 11.84GIGNFTSLSGRR3340 pKa = 11.84AGAPGFTGSTGGFLLGYY3357 pKa = 8.72DD3358 pKa = 4.27QPFADD3363 pKa = 3.59NAYY3366 pKa = 9.92LGVAGGYY3373 pKa = 10.17LNSSVDD3379 pKa = 3.49EE3380 pKa = 4.74HH3381 pKa = 6.25STSSGSIEE3389 pKa = 3.99SARR3392 pKa = 11.84ISLYY3396 pKa = 10.5GGVVLGPSLFTATAGYY3412 pKa = 9.93AHH3414 pKa = 7.47DD3415 pKa = 3.49WMRR3418 pKa = 11.84TEE3420 pKa = 4.85RR3421 pKa = 11.84GIAGIGTARR3430 pKa = 11.84EE3431 pKa = 3.98SHH3433 pKa = 6.39GGDD3436 pKa = 2.97EE3437 pKa = 4.48ATFAGQWSMPLAIPGLAGGSATLTPKK3463 pKa = 10.7VGIQYY3468 pKa = 9.53VHH3470 pKa = 6.99LSEE3473 pKa = 6.05GGFTDD3478 pKa = 3.91TGAGGFDD3485 pKa = 3.92LGADD3489 pKa = 3.54SRR3491 pKa = 11.84SADD3494 pKa = 3.02SFQPYY3499 pKa = 9.98FGFALGQRR3507 pKa = 11.84FTTRR3511 pKa = 11.84GGTDD3515 pKa = 2.94ITPEE3519 pKa = 3.87LRR3521 pKa = 11.84VGYY3524 pKa = 10.21AYY3526 pKa = 7.78EE3527 pKa = 4.04TLSNARR3533 pKa = 11.84RR3534 pKa = 11.84MTVIAADD3541 pKa = 3.8GTAFPVTGVPPSRR3554 pKa = 11.84NQLTAGIGVTMSAGPNLSFYY3574 pKa = 11.33ANYY3577 pKa = 10.41DD3578 pKa = 3.68AILPVANTRR3587 pKa = 11.84SQTFQAGLRR3596 pKa = 11.84WRR3598 pKa = 11.84FF3599 pKa = 3.12

Molecular weight: 340.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H7H559|A0A1H7H559_9BURK Chromosome segregation DNA-binding protein OS=Paraburkholderia caballeronis OX=416943 GN=SAMN05192542_10268 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 8.74TAGGRR28 pKa = 11.84KK29 pKa = 9.04VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LAII44 pKa = 4.0

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6189

0

6189

2028450

29

6079

327.8

35.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.413 ± 0.044

0.898 ± 0.01

5.811 ± 0.028

4.813 ± 0.037

3.621 ± 0.02

8.429 ± 0.046

2.292 ± 0.016

4.424 ± 0.023

2.619 ± 0.027

10.191 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.177 ± 0.015

2.681 ± 0.024

5.279 ± 0.024

3.5 ± 0.017

7.38 ± 0.045

5.449 ± 0.029

5.408 ± 0.041

7.859 ± 0.028

1.371 ± 0.012

2.386 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski