Staphylococcus phage StB20-like

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N9SH85|A0A0N9SH85_9CAUD Endodeoxyribonuclease RusA OS=Staphylococcus phage StB20-like OX=1732064 GN=StB20like_00033 PE=4 SV=1
MM1 pKa = 7.73SMTNSNVDD9 pKa = 2.78TGQQDD14 pKa = 3.78MVVEE18 pKa = 5.08GYY20 pKa = 10.99AIIFNSMSDD29 pKa = 3.4DD30 pKa = 3.43LGGFRR35 pKa = 11.84EE36 pKa = 4.34IVAPNALDD44 pKa = 3.93GVDD47 pKa = 3.88VSDD50 pKa = 3.95VKK52 pKa = 11.21CLINHH57 pKa = 6.81DD58 pKa = 4.04FSYY61 pKa = 11.33VIGRR65 pKa = 11.84TQAGTLEE72 pKa = 4.17LQVDD76 pKa = 3.92EE77 pKa = 4.85KK78 pKa = 11.46GLYY81 pKa = 8.73FKK83 pKa = 10.94CHH85 pKa = 6.57LPNTSYY91 pKa = 11.46ARR93 pKa = 11.84DD94 pKa = 3.19IYY96 pKa = 11.41EE97 pKa = 4.31NIKK100 pKa = 10.32AGNVNQCSFFYY111 pKa = 10.23TLPPNDD117 pKa = 3.19STARR121 pKa = 11.84TWQNIDD127 pKa = 3.08NEE129 pKa = 4.46YY130 pKa = 10.44VQTINKK136 pKa = 8.74IDD138 pKa = 3.73EE139 pKa = 5.03LIEE142 pKa = 3.92VSIVTIPAYY151 pKa = 9.86KK152 pKa = 9.26DD153 pKa = 3.02TSVEE157 pKa = 3.88VGQRR161 pKa = 11.84ARR163 pKa = 11.84DD164 pKa = 3.56LKK166 pKa = 10.89KK167 pKa = 10.66FKK169 pKa = 10.37QLEE172 pKa = 4.12QMKK175 pKa = 10.15IALDD179 pKa = 3.8LEE181 pKa = 4.47SLRR184 pKa = 11.84FEE186 pKa = 4.44TT187 pKa = 5.56

Molecular weight: 21.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N9SKK8|A0A0N9SKK8_9CAUD Transcriptional activator RinB OS=Staphylococcus phage StB20-like OX=1732064 GN=StB20like_00029 PE=4 SV=1
MM1 pKa = 7.45LPFSVFLLKK10 pKa = 10.28KK11 pKa = 9.75VKK13 pKa = 10.2NNKK16 pKa = 8.41GRR18 pKa = 11.84HH19 pKa = 4.26ATRR22 pKa = 11.84KK23 pKa = 9.45IILEE27 pKa = 4.34IILDD31 pKa = 3.43VTYY34 pKa = 11.22NVFKK38 pKa = 10.09ITRR41 pKa = 11.84EE42 pKa = 3.85GLALGPRR49 pKa = 11.84MGVGEE54 pKa = 4.3SS55 pKa = 3.24

Molecular weight: 6.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

12327

37

1570

208.9

23.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.857 ± 0.551

0.535 ± 0.096

5.979 ± 0.353

7.374 ± 0.711

4.121 ± 0.229

5.808 ± 0.667

1.882 ± 0.155

7.828 ± 0.312

9.581 ± 0.364

7.812 ± 0.321

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.555 ± 0.241

7.269 ± 0.304

2.985 ± 0.17

3.991 ± 0.309

3.724 ± 0.172

5.963 ± 0.311

5.695 ± 0.257

5.881 ± 0.208

1.079 ± 0.149

4.08 ± 0.309
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski