Lactococcus phage phiQ1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Teubervirus; Lactococcus virus phiQ1

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A455VJF7|A0A455VJF7_9CAUD Uncharacterized protein OS=Lactococcus phage phiQ1 OX=2488571 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 10.47LKK4 pKa = 10.86LIDD7 pKa = 3.51VRR9 pKa = 11.84LDD11 pKa = 3.35EE12 pKa = 5.49DD13 pKa = 3.55FHH15 pKa = 9.63AEE17 pKa = 4.57FGTCDD22 pKa = 3.13LCMSTGYY29 pKa = 10.3AAQPTYY35 pKa = 10.6IFEE38 pKa = 4.66DD39 pKa = 3.69EE40 pKa = 3.99NGIRR44 pKa = 11.84TEE46 pKa = 3.92VEE48 pKa = 3.94GYY50 pKa = 7.58YY51 pKa = 9.85WSWGDD56 pKa = 3.39YY57 pKa = 11.39DD58 pKa = 4.81EE59 pKa = 6.32INVNNVIDD67 pKa = 4.16FAAWVAEE74 pKa = 3.84QDD76 pKa = 3.85FSDD79 pKa = 3.64TLKK82 pKa = 10.57EE83 pKa = 4.17VEE85 pKa = 4.68VRR87 pKa = 11.84WDD89 pKa = 3.28TYY91 pKa = 9.5NQHH94 pKa = 7.2SIFDD98 pKa = 4.08WGWLRR103 pKa = 11.84AIVQAYY109 pKa = 8.83NEE111 pKa = 4.28KK112 pKa = 10.62LNIVEE117 pKa = 5.04GVITYY122 pKa = 9.34DD123 pKa = 3.07

Molecular weight: 14.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A455VEG5|A0A455VEG5_9CAUD Uncharacterized protein OS=Lactococcus phage phiQ1 OX=2488571 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.0EE3 pKa = 3.23IWKK6 pKa = 10.14DD7 pKa = 3.48VIDD10 pKa = 3.95YY11 pKa = 10.09EE12 pKa = 5.41GYY14 pKa = 9.63YY15 pKa = 9.69QVSNLGGFRR24 pKa = 11.84SLEE27 pKa = 3.93RR28 pKa = 11.84KK29 pKa = 9.31SRR31 pKa = 11.84SGKK34 pKa = 5.84TLKK37 pKa = 10.92AKK39 pKa = 10.54VLTGTINQAGYY50 pKa = 10.42VAVHH54 pKa = 6.45LSKK57 pKa = 11.2DD58 pKa = 3.35GTLKK62 pKa = 10.59KK63 pKa = 10.48DD64 pKa = 3.44LLHH67 pKa = 6.44RR68 pKa = 11.84LVMKK72 pKa = 10.82AFVEE76 pKa = 4.32NPNNYY81 pKa = 8.9PQVNHH86 pKa = 6.64IDD88 pKa = 3.61EE89 pKa = 4.89NKK91 pKa = 9.71TNNCITNLEE100 pKa = 4.29WVSPKK105 pKa = 10.49QNANHH110 pKa = 5.86GTRR113 pKa = 11.84NQRR116 pKa = 11.84VVEE119 pKa = 3.97TTIRR123 pKa = 11.84NNKK126 pKa = 8.62LGKK129 pKa = 9.48PVTNGFKK136 pKa = 9.92TFQSIRR142 pKa = 11.84GAGRR146 pKa = 11.84ITGISSSGINACLRR160 pKa = 11.84GLQKK164 pKa = 9.77TAGGFTWRR172 pKa = 11.84YY173 pKa = 7.49MNEE176 pKa = 3.62

Molecular weight: 19.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

18207

45

1309

211.7

23.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.992 ± 0.473

1.126 ± 0.16

6.481 ± 0.22

7.113 ± 0.434

4.048 ± 0.176

6.119 ± 0.49

1.637 ± 0.144

6.585 ± 0.183

7.08 ± 0.317

8.464 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.801 ± 0.093

6.261 ± 0.184

3.175 ± 0.187

3.916 ± 0.209

3.757 ± 0.23

6.283 ± 0.328

7.113 ± 0.304

6.816 ± 0.217

1.126 ± 0.118

4.108 ± 0.251
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski