Elizabethkingia sp. JS20170427COW

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Weeksellaceae; Elizabethkingia; unclassified Elizabethkingia

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2276 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B7UDV0|A0A5B7UDV0_9FLAO D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase OS=Elizabethkingia sp. JS20170427COW OX=2583851 GN=dacB PE=3 SV=1
MM1 pKa = 7.72RR2 pKa = 11.84NDD4 pKa = 3.15IPTNNQEE11 pKa = 4.23KK12 pKa = 10.87LEE14 pKa = 4.04YY15 pKa = 9.96ALKK18 pKa = 10.72GLYY21 pKa = 9.56DD22 pKa = 3.6VYY24 pKa = 11.27DD25 pKa = 4.02PEE27 pKa = 5.03VGLNVVDD34 pKa = 5.21LGLIYY39 pKa = 10.13EE40 pKa = 4.2IYY42 pKa = 10.28FYY44 pKa = 11.13EE45 pKa = 4.51EE46 pKa = 4.03EE47 pKa = 4.14EE48 pKa = 4.63KK49 pKa = 10.38MVSTLMTLTTQFCPMGEE66 pKa = 4.57AITNDD71 pKa = 3.12VTEE74 pKa = 4.84SLTQAFPGYY83 pKa = 9.54KK84 pKa = 9.54INVEE88 pKa = 4.17LTFDD92 pKa = 4.17PAWDD96 pKa = 3.74YY97 pKa = 11.66TKK99 pKa = 9.35ITPEE103 pKa = 3.79GKK105 pKa = 10.15EE106 pKa = 3.84FLGQQ110 pKa = 3.27

Molecular weight: 12.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B7UFT1|A0A5B7UFT1_9FLAO Nucleoside triphosphate pyrophosphohydrolase OS=Elizabethkingia sp. JS20170427COW OX=2583851 GN=mazG PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.55RR3 pKa = 11.84TFQPSEE9 pKa = 3.58RR10 pKa = 11.84KK11 pKa = 9.37KK12 pKa = 10.2RR13 pKa = 11.84NKK15 pKa = 9.71HH16 pKa = 4.09GFRR19 pKa = 11.84EE20 pKa = 4.33RR21 pKa = 11.84MSTPNGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.76GRR39 pKa = 11.84KK40 pKa = 8.66RR41 pKa = 11.84LTVSVARR48 pKa = 11.84AKK50 pKa = 10.51RR51 pKa = 3.35

Molecular weight: 6.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2276

0

2276

784826

38

2351

344.8

39.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.385 ± 0.05

0.721 ± 0.014

5.143 ± 0.029

6.79 ± 0.055

5.22 ± 0.046

6.367 ± 0.044

1.885 ± 0.024

7.948 ± 0.051

8.224 ± 0.047

9.39 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.273 ± 0.02

5.903 ± 0.05

3.481 ± 0.026

4.026 ± 0.032

3.344 ± 0.027

6.43 ± 0.042

5.211 ± 0.031

5.929 ± 0.039

1.078 ± 0.015

4.251 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski