Alpaca respiratory coronavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Duvinacovirus; Human coronavirus 229E

Average proteome isoelectric point is 7.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I1VWF6|I1VWF6_CVH22 Truncated ORF4 OS=Alpaca respiratory coronavirus OX=1176035 GN=ORF4 PE=4 SV=1
MM1 pKa = 7.09FVLVAYY7 pKa = 10.03ALLHH11 pKa = 6.13IAGCQTINVLNTSHH25 pKa = 6.59SVCNGCVGYY34 pKa = 10.09SEE36 pKa = 5.49NVFAVEE42 pKa = 4.01SGGYY46 pKa = 9.24IPSDD50 pKa = 3.14FAFNNWFLLTNTSSLVDD67 pKa = 3.51GVVRR71 pKa = 11.84SFQPLLLNCLWSVSGSRR88 pKa = 11.84FTTGFVYY95 pKa = 10.41FNGTGRR101 pKa = 11.84GDD103 pKa = 3.63CKK105 pKa = 10.87GFSSNVLSDD114 pKa = 3.61VIRR117 pKa = 11.84YY118 pKa = 8.25NINFEE123 pKa = 4.16EE124 pKa = 4.17NLRR127 pKa = 11.84RR128 pKa = 11.84GTILFKK134 pKa = 10.5TSFGVVVFYY143 pKa = 8.58CTNNTLVSGDD153 pKa = 3.46AYY155 pKa = 10.18IPFGTVLGNFYY166 pKa = 10.84CFVNTTIGNEE176 pKa = 4.22TTSSFVGALPKK187 pKa = 9.64TVRR190 pKa = 11.84EE191 pKa = 4.1FVISRR196 pKa = 11.84TGHH199 pKa = 6.13FYY201 pKa = 10.78INGYY205 pKa = 10.46RR206 pKa = 11.84YY207 pKa = 7.33FTLGNVEE214 pKa = 3.93AVNFNVTNAEE224 pKa = 4.21TTFCTVALASYY235 pKa = 11.29ADD237 pKa = 3.47VLVNVSQTAIANIIYY252 pKa = 9.57CNSVINRR259 pKa = 11.84LRR261 pKa = 11.84CDD263 pKa = 3.35QLSFDD268 pKa = 4.48VPDD271 pKa = 4.15GFYY274 pKa = 9.65STSPIQSVEE283 pKa = 4.08LPVSIVSLPVYY294 pKa = 9.8HH295 pKa = 6.85KK296 pKa = 9.52HH297 pKa = 4.94TFIVLYY303 pKa = 10.81VDD305 pKa = 5.71FKK307 pKa = 9.27PQSGAGTCYY316 pKa = 9.24NCRR319 pKa = 11.84PSVVNITLANFNEE332 pKa = 4.85TKK334 pKa = 10.76GPLCVEE340 pKa = 4.67TSHH343 pKa = 6.75FTTKK347 pKa = 10.41YY348 pKa = 7.97VASNVGRR355 pKa = 11.84WSASINTGNCPFSFGKK371 pKa = 10.56VNNFVKK377 pKa = 10.67FGSVCFSLKK386 pKa = 10.45DD387 pKa = 3.56IPGGCAMPIVANFAYY402 pKa = 9.7INSYY406 pKa = 9.54TIGSLYY412 pKa = 10.74VSWSDD417 pKa = 3.16GDD419 pKa = 4.55GITGVPKK426 pKa = 10.08PVEE429 pKa = 4.35GVSSFMNVTLNKK441 pKa = 7.67CTKK444 pKa = 9.28YY445 pKa = 10.78NIYY448 pKa = 10.19DD449 pKa = 3.42VSGVGVIRR457 pKa = 11.84ISNDD461 pKa = 2.59TFLNGITYY469 pKa = 9.16TSTSGNLLGFKK480 pKa = 10.35DD481 pKa = 3.72VTNGIIYY488 pKa = 10.18SITPCNPPDD497 pKa = 3.49QLVVYY502 pKa = 8.2QQAVVGAMLSEE513 pKa = 4.31NSSSYY518 pKa = 10.69GFSNVVEE525 pKa = 4.56LPNFFYY531 pKa = 10.92ASNGTYY537 pKa = 10.3NCTDD541 pKa = 2.86AVLTYY546 pKa = 11.05SSFGVCADD554 pKa = 3.43GSIIAVQPRR563 pKa = 11.84NVSYY567 pKa = 10.96DD568 pKa = 3.28GVSAIVTANLSIPSNWTTSVQVEE591 pKa = 4.12YY592 pKa = 10.83LQITSTPILVDD603 pKa = 3.61CSTYY607 pKa = 10.14VCNGNVRR614 pKa = 11.84CVEE617 pKa = 4.02LLKK620 pKa = 10.78QYY622 pKa = 10.53TSACKK627 pKa = 9.83TIEE630 pKa = 4.06DD631 pKa = 4.09ALRR634 pKa = 11.84ISAMLEE640 pKa = 3.94SADD643 pKa = 3.73VGEE646 pKa = 4.64MLTFDD651 pKa = 3.92EE652 pKa = 5.67KK653 pKa = 11.5AFTLANVSSFGDD665 pKa = 3.69YY666 pKa = 10.6NLSSVIPSLPTSGSRR681 pKa = 11.84VAGRR685 pKa = 11.84SAIEE689 pKa = 5.09DD690 pKa = 3.29ILFSKK695 pKa = 10.76VVTSGLGTVDD705 pKa = 4.5ADD707 pKa = 3.86YY708 pKa = 11.22KK709 pKa = 11.03KK710 pKa = 9.54CTKK713 pKa = 10.13GLSIADD719 pKa = 4.44LACAQYY725 pKa = 11.34YY726 pKa = 9.2NGIMVLPGVADD737 pKa = 4.01AEE739 pKa = 4.12RR740 pKa = 11.84MAMYY744 pKa = 9.17TGSLIGGIALGGLTSAAAIPFSLALQARR772 pKa = 11.84LNYY775 pKa = 9.74VALQTDD781 pKa = 4.22VLQEE785 pKa = 3.94NQKK788 pKa = 9.9ILAASFNKK796 pKa = 10.34AMTNIVDD803 pKa = 3.87AFTGVNDD810 pKa = 6.2AITQTSQAIQTVATALNKK828 pKa = 10.09IQDD831 pKa = 3.83VVNQQGNALNHH842 pKa = 5.93LTSQLRR848 pKa = 11.84QNFQAISSSIQAIYY862 pKa = 10.75DD863 pKa = 3.62RR864 pKa = 11.84LDD866 pKa = 3.71TIQADD871 pKa = 3.81QQVDD875 pKa = 3.53RR876 pKa = 11.84LITGRR881 pKa = 11.84LAALNAFVAQTLTKK895 pKa = 8.19YY896 pKa = 9.39TEE898 pKa = 4.13VRR900 pKa = 11.84ASRR903 pKa = 11.84QLAQQKK909 pKa = 10.1VNEE912 pKa = 4.46CVKK915 pKa = 10.52SQSNRR920 pKa = 11.84YY921 pKa = 7.84GFCGNGTHH929 pKa = 6.84IFSIVNSAPEE939 pKa = 4.07GLVFLHH945 pKa = 6.06TVLLPTQYY953 pKa = 11.19KK954 pKa = 10.38DD955 pKa = 3.27VEE957 pKa = 4.35AWSGLCVDD965 pKa = 3.87NTNGYY970 pKa = 7.62VLRR973 pKa = 11.84QPNLALYY980 pKa = 10.59KK981 pKa = 9.95EE982 pKa = 4.32GNYY985 pKa = 10.13YY986 pKa = 10.71RR987 pKa = 11.84ITSRR991 pKa = 11.84FMFEE995 pKa = 3.65PRR997 pKa = 11.84IPTMADD1003 pKa = 3.53FVQIEE1008 pKa = 4.38KK1009 pKa = 11.09CNVTFVNISRR1019 pKa = 11.84SEE1021 pKa = 3.96LQTIVPEE1028 pKa = 4.33YY1029 pKa = 10.5IDD1031 pKa = 3.57VNKK1034 pKa = 9.35TLQEE1038 pKa = 4.88LIDD1041 pKa = 4.09KK1042 pKa = 9.89LPNYY1046 pKa = 7.3TVPDD1050 pKa = 4.22LGIDD1054 pKa = 3.54QYY1056 pKa = 12.07NQTILNLTSEE1066 pKa = 4.16ISTLEE1071 pKa = 4.02NKK1073 pKa = 9.98SAEE1076 pKa = 4.14LNYY1079 pKa = 9.72TVQRR1083 pKa = 11.84LQTLIDD1089 pKa = 4.6NINSTLVDD1097 pKa = 4.5LKK1099 pKa = 10.17WLNRR1103 pKa = 11.84VEE1105 pKa = 5.47TYY1107 pKa = 10.68LKK1109 pKa = 9.22WPWWVWLCISVVLIFVVSILLLCCCSTGCCGFFSCLASSTRR1150 pKa = 11.84GCCEE1154 pKa = 3.91STKK1157 pKa = 10.6LPYY1160 pKa = 10.38YY1161 pKa = 10.29DD1162 pKa = 3.56VEE1164 pKa = 5.49KK1165 pKa = 10.86IHH1167 pKa = 6.47IQQ1169 pKa = 3.23

Molecular weight: 127.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I1VWF7|I1VWF7_CVH22 Envelope small membrane protein OS=Alpaca respiratory coronavirus OX=1176035 GN=E PE=3 SV=1
MM1 pKa = 7.62ALGLFTLQIEE11 pKa = 4.69SAVNQSLSKK20 pKa = 10.41SKK22 pKa = 10.65VSAVVSRR29 pKa = 11.84QVIQDD34 pKa = 3.1VRR36 pKa = 11.84AAAVTFNLLAYY47 pKa = 8.89TLMSLFVVYY56 pKa = 9.16FAFFQSKK63 pKa = 8.24II64 pKa = 3.68

Molecular weight: 7.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

12866

64

6762

1608.3

178.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.917 ± 0.169

3.311 ± 0.396

5.41 ± 0.443

4.205 ± 0.155

5.689 ± 0.217

6.451 ± 0.135

1.687 ± 0.127

5.099 ± 0.254

6.008 ± 0.515

8.651 ± 0.226

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.153 ± 0.224

5.682 ± 0.283

3.397 ± 0.39

2.899 ± 0.413

3.257 ± 0.313

7.205 ± 0.559

5.938 ± 0.415

10.353 ± 0.323

1.36 ± 0.136

4.329 ± 0.207
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski