Chitinophagaceae bacterium BSSC1

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; unclassified Chitinophagaceae

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3607 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A257I1M3|A0A257I1M3_9BACT Sortilin-Vps10 domain-containing protein OS=Chitinophagaceae bacterium BSSC1 OX=2015574 GN=CFE25_05275 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.14FYY4 pKa = 11.1SLDD7 pKa = 3.54SKK9 pKa = 10.45FTFGKK14 pKa = 10.87YY15 pKa = 9.85SDD17 pKa = 3.55NTIAEE22 pKa = 4.16VLILDD27 pKa = 3.68KK28 pKa = 11.07AYY30 pKa = 11.1VEE32 pKa = 4.0WCLINLNHH40 pKa = 6.42FVIDD44 pKa = 3.79DD45 pKa = 3.86DD46 pKa = 5.07VIEE49 pKa = 4.7EE50 pKa = 4.36VQSHH54 pKa = 5.77NPSFQLSKK62 pKa = 10.82EE63 pKa = 3.91SDD65 pKa = 3.32EE66 pKa = 4.53ARR68 pKa = 11.84VAKK71 pKa = 10.35LDD73 pKa = 3.64EE74 pKa = 4.66YY75 pKa = 11.54YY76 pKa = 10.64EE77 pKa = 4.17EE78 pKa = 3.94QASYY82 pKa = 11.33DD83 pKa = 3.81EE84 pKa = 5.26EE85 pKa = 5.41DD86 pKa = 4.4FEE88 pKa = 6.39DD89 pKa = 5.02PEE91 pKa = 5.85DD92 pKa = 4.86DD93 pKa = 3.86YY94 pKa = 12.11GSSGEE99 pKa = 4.09KK100 pKa = 9.95YY101 pKa = 8.76GWYY104 pKa = 10.43NGFSDD109 pKa = 5.54DD110 pKa = 5.56AIDD113 pKa = 5.09DD114 pKa = 4.12AFEE117 pKa = 4.76GDD119 pKa = 5.9PMNTWNVDD127 pKa = 2.92

Molecular weight: 14.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A257HYU4|A0A257HYU4_9BACT 30S ribosomal protein S5 OS=Chitinophagaceae bacterium BSSC1 OX=2015574 GN=rpsE PE=3 SV=1
MM1 pKa = 7.76PPKK4 pKa = 10.33QPWAILGHH12 pKa = 5.0GVKK15 pKa = 10.4LATAYY20 pKa = 9.75HH21 pKa = 6.05AQRR24 pKa = 11.84GIPVEE29 pKa = 3.86HH30 pKa = 6.71SRR32 pKa = 11.84AISVGSVKK40 pKa = 10.73GNLNAAIATAGGCKK54 pKa = 7.59WRR56 pKa = 11.84RR57 pKa = 11.84DD58 pKa = 3.54VLMAHH63 pKa = 7.39PP64 pKa = 4.72

Molecular weight: 6.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3607

0

3607

1338094

29

3700

371.0

41.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.603 ± 0.04

0.871 ± 0.012

5.036 ± 0.028

5.392 ± 0.046

4.969 ± 0.027

7.005 ± 0.038

1.921 ± 0.021

7.154 ± 0.032

7.096 ± 0.039

9.601 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.497 ± 0.023

5.629 ± 0.043

3.947 ± 0.025

4.299 ± 0.031

3.504 ± 0.028

6.44 ± 0.037

5.596 ± 0.055

6.133 ± 0.031

1.368 ± 0.018

3.941 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski