Corynebacterium phage IME1320_01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6JQB5|A0A1W6JQB5_9CAUD Uncharacterized protein OS=Corynebacterium phage IME1320_01 OX=1965535 PE=4 SV=1
MM1 pKa = 7.94ALRR4 pKa = 11.84KK5 pKa = 9.75SYY7 pKa = 8.44PTDD10 pKa = 4.44DD11 pKa = 4.39DD12 pKa = 5.13LEE14 pKa = 4.42DD15 pKa = 3.93VEE17 pKa = 5.59ISEE20 pKa = 4.85FPCMRR25 pKa = 11.84DD26 pKa = 3.07AVRR29 pKa = 11.84KK30 pKa = 9.35SVEE33 pKa = 4.26TADD36 pKa = 3.8HH37 pKa = 7.23LEE39 pKa = 4.57DD40 pKa = 3.8TDD42 pKa = 3.83EE43 pKa = 6.24ADD45 pKa = 3.4IQLAYY50 pKa = 10.59QLADD54 pKa = 3.68IIDD57 pKa = 4.19DD58 pKa = 3.67ARR60 pKa = 11.84EE61 pKa = 4.16SGDD64 pKa = 3.38PDD66 pKa = 5.4AIHH69 pKa = 6.18KK70 pKa = 7.63TAFGPMPTLHH80 pKa = 7.04KK81 pKa = 10.82VLTSLGLNPEE91 pKa = 4.2GRR93 pKa = 11.84DD94 pKa = 3.39KK95 pKa = 11.51LGLNTQEE102 pKa = 6.04DD103 pKa = 4.26DD104 pKa = 4.09EE105 pKa = 4.76DD106 pKa = 3.59WW107 pKa = 4.79

Molecular weight: 11.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6JQ93|A0A1W6JQ93_9CAUD Uncharacterized protein OS=Corynebacterium phage IME1320_01 OX=1965535 PE=4 SV=1
MM1 pKa = 7.54GPNPFTKK8 pKa = 10.25KK9 pKa = 9.98EE10 pKa = 4.04STMKK14 pKa = 10.56LIKK17 pKa = 10.52ALFHH21 pKa = 6.95RR22 pKa = 11.84GRR24 pKa = 11.84HH25 pKa = 4.82RR26 pKa = 11.84KK27 pKa = 8.59PRR29 pKa = 11.84TRR31 pKa = 11.84TFWVVWRR38 pKa = 4.56

Molecular weight: 4.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12430

37

1636

234.5

25.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.539 ± 0.645

0.611 ± 0.102

6.629 ± 0.422

6.951 ± 0.322

3.146 ± 0.22

8.286 ± 0.56

2.188 ± 0.241

4.602 ± 0.188

5.221 ± 0.336

8.238 ± 0.406

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.172 ± 0.142

3.057 ± 0.174

5.004 ± 0.3

3.652 ± 0.199

6.114 ± 0.426

6.05 ± 0.441

6.09 ± 0.269

6.79 ± 0.224

2.132 ± 0.178

2.526 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski