Mycobacterium phage L5 (Mycobacteriophage L5)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 90 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q05305|VG88_BPML5 Gene 88 protein OS=Mycobacterium phage L5 OX=31757 GN=88 PE=4 SV=1
MM1 pKa = 8.2SFEE4 pKa = 3.85TWMAALDD11 pKa = 4.15GYY13 pKa = 7.95MTEE16 pKa = 4.32TFGLGYY22 pKa = 10.06QDD24 pKa = 3.26IADD27 pKa = 3.46WTYY30 pKa = 11.31RR31 pKa = 11.84DD32 pKa = 4.34AYY34 pKa = 10.96DD35 pKa = 4.69DD36 pKa = 3.96GLTFRR41 pKa = 11.84EE42 pKa = 4.2AAHH45 pKa = 6.41RR46 pKa = 11.84AINHH50 pKa = 5.69EE51 pKa = 4.24FLL53 pKa = 5.23

Molecular weight: 6.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q05252|VG41_BPML5 Gene 41 protein OS=Mycobacterium phage L5 OX=31757 GN=41 PE=4 SV=1
MM1 pKa = 7.25SWAGSGRR8 pKa = 11.84RR9 pKa = 11.84QEE11 pKa = 5.38LPEE14 pKa = 4.38DD15 pKa = 3.29WEE17 pKa = 4.35LNYY20 pKa = 10.51RR21 pKa = 11.84LPVLSAANWLCQINGPGCVRR41 pKa = 11.84AATDD45 pKa = 2.9VDD47 pKa = 4.26HH48 pKa = 7.46IKK50 pKa = 10.61RR51 pKa = 11.84GNDD54 pKa = 3.11HH55 pKa = 6.61SRR57 pKa = 11.84SNLQAACHH65 pKa = 5.36VCHH68 pKa = 6.5GKK70 pKa = 10.35KK71 pKa = 9.92SAAEE75 pKa = 3.95GVARR79 pKa = 11.84RR80 pKa = 11.84RR81 pKa = 11.84EE82 pKa = 3.69LRR84 pKa = 11.84ARR86 pKa = 11.84RR87 pKa = 11.84KK88 pKa = 9.79RR89 pKa = 11.84PPEE92 pKa = 3.6RR93 pKa = 11.84HH94 pKa = 5.7PGRR97 pKa = 11.84RR98 pKa = 3.38

Molecular weight: 11.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

90

0

90

15843

37

837

176.0

19.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.676 ± 0.405

0.985 ± 0.141

6.444 ± 0.268

7.031 ± 0.293

3.383 ± 0.168

7.909 ± 0.391

2.241 ± 0.212

4.974 ± 0.159

4.993 ± 0.244

8.142 ± 0.303

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.506 ± 0.133

3.352 ± 0.192

5.34 ± 0.241

3.585 ± 0.212

6.444 ± 0.373

4.961 ± 0.196

5.857 ± 0.246

7.158 ± 0.224

1.995 ± 0.128

3.023 ± 0.198
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski