Edwardsiella phage eiAU-183

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Eiauvirus; unclassified Eiauvirus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W0LI09|W0LI09_9CAUD Uncharacterized protein OS=Edwardsiella phage eiAU-183 OX=1391659 GN=O197_08 PE=4 SV=1
MM1 pKa = 7.47TSYY4 pKa = 10.83IDD6 pKa = 3.62QSAKK10 pKa = 10.28LDD12 pKa = 3.65PSGRR16 pKa = 11.84IVLVEE21 pKa = 3.74VDD23 pKa = 3.64ASEE26 pKa = 4.69FGAGVHH32 pKa = 6.01RR33 pKa = 11.84MHH35 pKa = 6.92YY36 pKa = 10.89APFPHH41 pKa = 6.45SAAEE45 pKa = 3.97IEE47 pKa = 4.24AAAGDD52 pKa = 4.06EE53 pKa = 4.51AKK55 pKa = 10.68LGPKK59 pKa = 9.82PIYY62 pKa = 10.29FGGLMFDD69 pKa = 3.76FWPFSVSGLSLSTEE83 pKa = 3.99QAATPTITVSNLAGYY98 pKa = 10.13LSRR101 pKa = 11.84LCLDD105 pKa = 3.35YY106 pKa = 11.18RR107 pKa = 11.84DD108 pKa = 4.9LINAKK113 pKa = 9.52VRR115 pKa = 11.84VIYY118 pKa = 9.39TYY120 pKa = 11.33AEE122 pKa = 3.97YY123 pKa = 11.05LDD125 pKa = 4.42ARR127 pKa = 11.84NFPDD131 pKa = 4.22GNPNADD137 pKa = 4.14PDD139 pKa = 3.83ACSYY143 pKa = 7.6QTFWVDD149 pKa = 3.23TKK151 pKa = 11.01SAEE154 pKa = 4.23DD155 pKa = 4.23DD156 pKa = 4.27EE157 pKa = 6.16SITWTLSSPADD168 pKa = 3.73LQGLKK173 pKa = 10.07IPTRR177 pKa = 11.84QITSLCTWAMRR188 pKa = 11.84GQYY191 pKa = 10.04RR192 pKa = 11.84SGDD195 pKa = 3.2GCTYY199 pKa = 10.85NGNAYY204 pKa = 10.1FDD206 pKa = 4.21AKK208 pKa = 10.37GNPVSDD214 pKa = 4.3PALDD218 pKa = 3.61RR219 pKa = 11.84CGGCYY224 pKa = 9.84SDD226 pKa = 3.62CVKK229 pKa = 10.81RR230 pKa = 11.84FGADD234 pKa = 2.93MADD237 pKa = 3.66PKK239 pKa = 10.98AAALDD244 pKa = 3.89FGGFLAAQLINRR256 pKa = 4.72

Molecular weight: 27.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W0LI36|W0LI36_9CAUD Putative lysis accessory protein OS=Edwardsiella phage eiAU-183 OX=1391659 GN=O197_38 PE=4 SV=1
MM1 pKa = 7.29QKK3 pKa = 10.59LIAYY7 pKa = 6.58ITAPMRR13 pKa = 11.84AIMKK17 pKa = 9.08YY18 pKa = 9.21RR19 pKa = 11.84RR20 pKa = 11.84EE21 pKa = 4.02AKK23 pKa = 9.74RR24 pKa = 11.84RR25 pKa = 11.84AFLQSAIEE33 pKa = 3.98KK34 pKa = 10.43ADD36 pKa = 3.8YY37 pKa = 9.81QWRR40 pKa = 11.84TLPRR44 pKa = 11.84AKK46 pKa = 9.68MYY48 pKa = 9.72NAEE51 pKa = 4.05RR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84LEE56 pKa = 3.9RR57 pKa = 11.84QLDD60 pKa = 3.72SMRR63 pKa = 11.84CEE65 pKa = 4.19LEE67 pKa = 4.56LMDD70 pKa = 6.1GRR72 pKa = 11.84LQQ74 pKa = 3.63

Molecular weight: 9.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

12853

45

1203

238.0

26.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.137 ± 0.449

1.245 ± 0.143

6.403 ± 0.258

6.092 ± 0.262

3.143 ± 0.161

8.193 ± 0.435

1.657 ± 0.197

5.034 ± 0.205

5.306 ± 0.304

7.796 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.638 ± 0.182

3.626 ± 0.162

4.217 ± 0.298

3.781 ± 0.285

6.543 ± 0.337

5.415 ± 0.301

5.89 ± 0.219

6.481 ± 0.3

1.486 ± 0.124

2.918 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski