Walkabout Creek virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Sunrhavirus; Walkabout sunrhavirus

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0V5L0|A0A0A0V5L0_9RHAB Matrix protein OS=Walkabout Creek virus OX=1569258 GN=M PE=4 SV=1
MM1 pKa = 7.73LSTKK5 pKa = 8.01TLEE8 pKa = 4.25NLTKK12 pKa = 9.97YY13 pKa = 10.34QEE15 pKa = 4.51AIKK18 pKa = 10.79DD19 pKa = 3.68EE20 pKa = 4.54SDD22 pKa = 3.39PSEE25 pKa = 4.82DD26 pKa = 3.71CVGDD30 pKa = 4.12PEE32 pKa = 5.9SMPQLEE38 pKa = 4.27LSGGVSRR45 pKa = 11.84EE46 pKa = 3.94SSSQLVEE53 pKa = 4.68FMQKK57 pKa = 10.17SDD59 pKa = 3.35GANAGACDD67 pKa = 4.63VPYY70 pKa = 10.0PWAANSIHH78 pKa = 6.75NDD80 pKa = 3.05SFVDD84 pKa = 4.01PEE86 pKa = 4.42GEE88 pKa = 4.19EE89 pKa = 4.24CSDD92 pKa = 3.27RR93 pKa = 11.84TDD95 pKa = 4.3EE96 pKa = 4.46NDD98 pKa = 3.61CGEE101 pKa = 4.26NPYY104 pKa = 10.7SSRR107 pKa = 11.84RR108 pKa = 11.84ARR110 pKa = 11.84KK111 pKa = 9.42LYY113 pKa = 8.82EE114 pKa = 3.58QGYY117 pKa = 8.35EE118 pKa = 3.94DD119 pKa = 3.96GVRR122 pKa = 11.84EE123 pKa = 4.06IGRR126 pKa = 11.84QMRR129 pKa = 11.84QLQADD134 pKa = 4.45GFPFEE139 pKa = 4.86FEE141 pKa = 4.28YY142 pKa = 11.36VDD144 pKa = 3.66DD145 pKa = 4.08RR146 pKa = 11.84LRR148 pKa = 11.84IKK150 pKa = 10.62KK151 pKa = 9.37IVCDD155 pKa = 3.36QRR157 pKa = 11.84YY158 pKa = 9.29LIKK161 pKa = 10.64KK162 pKa = 8.99LIEE165 pKa = 4.33GGFFEE170 pKa = 4.5NLSPEE175 pKa = 4.22SEE177 pKa = 4.08IKK179 pKa = 9.49QKK181 pKa = 10.37QMEE184 pKa = 4.68SSNKK188 pKa = 9.67APPLHH193 pKa = 6.64QILKK197 pKa = 10.29DD198 pKa = 3.67SKK200 pKa = 11.11KK201 pKa = 10.19PNQKK205 pKa = 10.0CMQSPKK211 pKa = 10.12EE212 pKa = 3.86ASQPNSSMNSKK223 pKa = 10.08LGEE226 pKa = 3.72MDD228 pKa = 3.5YY229 pKa = 11.1RR230 pKa = 11.84LSFCGDD236 pKa = 3.42DD237 pKa = 3.52MLTMEE242 pKa = 5.06EE243 pKa = 4.68FIEE246 pKa = 4.26KK247 pKa = 10.33ANRR250 pKa = 11.84GLHH253 pKa = 5.04ICKK256 pKa = 10.08DD257 pKa = 3.73GKK259 pKa = 10.7NKK261 pKa = 9.72FVDD264 pKa = 4.71LEE266 pKa = 4.47DD267 pKa = 4.09FCPQKK272 pKa = 10.3IRR274 pKa = 11.84EE275 pKa = 4.2RR276 pKa = 11.84KK277 pKa = 9.63GDD279 pKa = 3.83LLTIEE284 pKa = 4.49EE285 pKa = 4.62WLACLYY291 pKa = 10.78

Molecular weight: 33.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0V5L7|A0A0A0V5L7_9RHAB Isoform of A0A0A0V394 C'' protein OS=Walkabout Creek virus OX=1569258 GN=P PE=4 SV=1
MM1 pKa = 7.44GRR3 pKa = 11.84TSSLTWKK10 pKa = 9.28TSVPKK15 pKa = 10.5RR16 pKa = 11.84FEE18 pKa = 3.96RR19 pKa = 11.84GKK21 pKa = 9.75ATFF24 pKa = 3.67

Molecular weight: 2.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

3

8

3703

24

2074

462.9

53.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.24 ± 1.072

2.106 ± 0.293

5.995 ± 0.72

6.859 ± 0.638

4.645 ± 0.404

5.455 ± 0.568

1.917 ± 0.423

8.237 ± 0.795

8.291 ± 0.497

9.776 ± 0.529

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.484 ± 0.295

5.59 ± 0.44

3.538 ± 0.319

3.268 ± 0.363

5.104 ± 0.305

8.102 ± 0.658

4.753 ± 0.563

4.213 ± 0.315

1.836 ± 0.252

3.592 ± 0.49
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski