Microviridae Fen51_42

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae; Microviridae Bog1249_12

Average proteome isoelectric point is 7.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G2UEF0|A0A0G2UEF0_9VIRU Replication protein VP4 OS=Microviridae Fen51_42 OX=1655655 PE=4 SV=1
MM1 pKa = 7.1KK2 pKa = 8.26TTYY5 pKa = 10.06DD6 pKa = 3.01IHH8 pKa = 7.79RR9 pKa = 11.84GWEE12 pKa = 3.89LEE14 pKa = 4.17YY15 pKa = 10.96TEE17 pKa = 5.33SSHH20 pKa = 6.45NFPSVTVPNLDD31 pKa = 3.22FTLAEE36 pKa = 4.55LIEE39 pKa = 4.17RR40 pKa = 11.84HH41 pKa = 5.26VRR43 pKa = 11.84GDD45 pKa = 3.51LTDD48 pKa = 4.1LGIGRR53 pKa = 11.84EE54 pKa = 4.12GLYY57 pKa = 10.69EE58 pKa = 5.05LDD60 pKa = 4.8DD61 pKa = 4.88DD62 pKa = 5.28SEE64 pKa = 4.84EE65 pKa = 4.02FDD67 pKa = 4.19SPDD70 pKa = 3.52FEE72 pKa = 5.95KK73 pKa = 10.68SAQMDD78 pKa = 3.8LVDD81 pKa = 3.4RR82 pKa = 11.84ARR84 pKa = 11.84FIEE87 pKa = 4.0EE88 pKa = 4.09TKK90 pKa = 10.25ISKK93 pKa = 10.43AMYY96 pKa = 9.3DD97 pKa = 3.1KK98 pKa = 10.92AYY100 pKa = 10.63RR101 pKa = 11.84EE102 pKa = 4.08AEE104 pKa = 4.1AAQKK108 pKa = 10.53VADD111 pKa = 4.06EE112 pKa = 4.57AAAASQRR119 pKa = 11.84AADD122 pKa = 3.75MAAAIQLQKK131 pKa = 10.26STDD134 pKa = 3.38KK135 pKa = 11.11TNEE138 pKa = 3.79KK139 pKa = 10.66

Molecular weight: 15.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G2UMF4|A0A0G2UMF4_9VIRU Uncharacterized protein OS=Microviridae Fen51_42 OX=1655655 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 10.75NFFKK6 pKa = 10.77KK7 pKa = 10.0GARR10 pKa = 11.84LIAYY14 pKa = 7.58RR15 pKa = 11.84RR16 pKa = 11.84CAIKK20 pKa = 10.24RR21 pKa = 11.84AEE23 pKa = 4.14SARR26 pKa = 11.84SGRR29 pKa = 11.84YY30 pKa = 8.13PSKK33 pKa = 9.92TKK35 pKa = 10.7RR36 pKa = 11.84NDD38 pKa = 2.99MTQEE42 pKa = 3.21WRR44 pKa = 11.84HH45 pKa = 4.83AHH47 pKa = 6.16LGGIQSGSLHH57 pKa = 6.81ACPAMAKK64 pKa = 10.07PLLTHH69 pKa = 7.03ILSCHH74 pKa = 6.17IYY76 pKa = 10.71ARR78 pKa = 11.84WSQLGSQLGPILTLLNVLRR97 pKa = 11.84GRR99 pKa = 11.84TGQGNTRR106 pKa = 11.84EE107 pKa = 4.34RR108 pKa = 11.84VNSVPP113 pKa = 4.03

Molecular weight: 12.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1271

113

507

254.2

28.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.868 ± 1.166

0.708 ± 0.42

4.799 ± 1.056

4.249 ± 1.085

4.17 ± 0.7

7.553 ± 0.968

2.046 ± 0.498

4.327 ± 0.113

5.586 ± 2.012

10.307 ± 1.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.203 ± 0.213

5.822 ± 1.179

4.091 ± 0.517

5.665 ± 0.972

5.822 ± 0.771

6.609 ± 0.647

6.845 ± 1.453

5.035 ± 0.92

1.338 ± 0.177

4.957 ± 1.1
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski