Bacillus phage Palmer

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Pagevirus; Bacillus virus Palmer

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5AFI4|A0A0C5AFI4_9CAUD Uncharacterized protein OS=Bacillus phage Palmer OX=1597966 GN=CPT_Palmer45 PE=4 SV=1
MM1 pKa = 7.32NLGEE5 pKa = 4.62AKK7 pKa = 10.34AHH9 pKa = 6.22ALALMAEE16 pKa = 4.16YY17 pKa = 10.45SIDD20 pKa = 3.45GLEE23 pKa = 4.55ISDD26 pKa = 5.36AEE28 pKa = 4.07NADD31 pKa = 3.5YY32 pKa = 10.84LKK34 pKa = 10.85RR35 pKa = 11.84MPLFAHH41 pKa = 7.09EE42 pKa = 4.19AQMDD46 pKa = 3.72ISARR50 pKa = 11.84SGVTAVMSFEE60 pKa = 4.81LVKK63 pKa = 10.66QDD65 pKa = 3.55DD66 pKa = 3.62VKK68 pKa = 11.45YY69 pKa = 11.14NYY71 pKa = 9.35IALPADD77 pKa = 3.74YY78 pKa = 10.93RR79 pKa = 11.84EE80 pKa = 4.09MKK82 pKa = 9.89YY83 pKa = 10.14VFWNEE88 pKa = 3.37EE89 pKa = 4.35LFTGNYY95 pKa = 10.01SILNNQIRR103 pKa = 11.84FNAGFDD109 pKa = 3.67GTIDD113 pKa = 3.92LYY115 pKa = 9.91YY116 pKa = 10.12WKK118 pKa = 10.42YY119 pKa = 8.95PQQLTTQTEE128 pKa = 3.81DD129 pKa = 3.35DD130 pKa = 4.27YY131 pKa = 11.74EE132 pKa = 4.44FEE134 pKa = 4.15IDD136 pKa = 4.39RR137 pKa = 11.84EE138 pKa = 4.24YY139 pKa = 11.32HH140 pKa = 6.37HH141 pKa = 7.26LIPYY145 pKa = 9.22YY146 pKa = 10.75LGGKK150 pKa = 9.44CMQDD154 pKa = 3.11EE155 pKa = 4.36NLDD158 pKa = 3.33IAEE161 pKa = 4.77RR162 pKa = 11.84LLSDD166 pKa = 3.7YY167 pKa = 11.02YY168 pKa = 11.67NRR170 pKa = 11.84LSDD173 pKa = 3.48VKK175 pKa = 10.15EE176 pKa = 3.77AHH178 pKa = 6.97EE179 pKa = 5.11DD180 pKa = 3.5GQEE183 pKa = 4.06RR184 pKa = 11.84IDD186 pKa = 4.04NIFTIFF192 pKa = 3.35

Molecular weight: 22.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5ACE6|A0A0C5ACE6_9CAUD Uncharacterized protein OS=Bacillus phage Palmer OX=1597966 GN=CPT_Palmer24 PE=4 SV=1
MM1 pKa = 7.31FGGGRR6 pKa = 11.84SEE8 pKa = 4.12KK9 pKa = 10.59DD10 pKa = 2.7IAAGRR15 pKa = 11.84FDD17 pKa = 3.52CSSYY21 pKa = 10.84VRR23 pKa = 11.84WAYY26 pKa = 10.5SQLGINLGPLTSTSTEE42 pKa = 3.87TLKK45 pKa = 11.4NKK47 pKa = 7.98GTAVKK52 pKa = 10.43GGLANAQPGDD62 pKa = 4.19LIFFDD67 pKa = 4.15TYY69 pKa = 10.87KK70 pKa = 11.0KK71 pKa = 10.0NGHH74 pKa = 4.46VVIYY78 pKa = 10.36LGNNQFIGAQGKK90 pKa = 7.34TGVGIVDD97 pKa = 4.61LNSSYY102 pKa = 11.45GSYY105 pKa = 10.07FKK107 pKa = 10.87KK108 pKa = 10.34RR109 pKa = 11.84FSGNIRR115 pKa = 11.84RR116 pKa = 11.84IAGGGNVGAALGGGGAAMSGAGTGTAYY143 pKa = 10.32RR144 pKa = 11.84QAPSNLMGPINNAAKK159 pKa = 10.29QYY161 pKa = 10.27GVNPNLIAAIIKK173 pKa = 10.22KK174 pKa = 10.2EE175 pKa = 4.13STFKK179 pKa = 11.0SGLTSSAGAKK189 pKa = 10.33GYY191 pKa = 7.86MQLMPATARR200 pKa = 11.84AMGVKK205 pKa = 10.22NPWDD209 pKa = 3.58TQQNINGGTKK219 pKa = 10.51YY220 pKa = 9.98IAQQLKK226 pKa = 8.97TYY228 pKa = 10.29KK229 pKa = 10.78NNIPLALAAYY239 pKa = 9.31NWGPGNLNKK248 pKa = 10.5AIRR251 pKa = 11.84KK252 pKa = 9.59AGGSKK257 pKa = 10.02DD258 pKa = 2.92WNQIRR263 pKa = 11.84RR264 pKa = 11.84FAPKK268 pKa = 8.44EE269 pKa = 3.76TRR271 pKa = 11.84DD272 pKa = 3.79YY273 pKa = 11.28VDD275 pKa = 5.67KK276 pKa = 11.37IMGWRR281 pKa = 3.39

Molecular weight: 29.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

12742

30

1678

254.8

28.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.06 ± 0.367

0.698 ± 0.135

5.556 ± 0.209

7.157 ± 0.521

4.136 ± 0.172

6.906 ± 0.339

1.601 ± 0.154

6.114 ± 0.395

8.28 ± 0.491

7.173 ± 0.341

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.833 ± 0.193

6.169 ± 0.245

3.704 ± 0.36

4.881 ± 0.582

4.128 ± 0.22

5.572 ± 0.23

5.972 ± 0.422

5.957 ± 0.238

1.24 ± 0.119

3.861 ± 0.354
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski