Hubei picorna-like virus 77

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KM48|A0A1L3KM48_9VIRU Calici_coat domain-containing protein OS=Hubei picorna-like virus 77 OX=1923161 PE=4 SV=1
MM1 pKa = 7.31SHH3 pKa = 7.51PGMSAAPAVSGDD15 pKa = 3.76PAISASTDD23 pKa = 3.16AAQLPLSTISTAPAPAAEE41 pKa = 5.44AIPHH45 pKa = 5.9TGSLNALDD53 pKa = 5.01PYY55 pKa = 11.03VKK57 pKa = 10.27EE58 pKa = 3.97QFISQGSFEE67 pKa = 4.65WTTADD72 pKa = 4.83LVGKK76 pKa = 9.95LIFSCPIHH84 pKa = 6.43PVNANPILAYY94 pKa = 10.28LSKK97 pKa = 10.38IYY99 pKa = 8.84NTWAGGLDD107 pKa = 4.3FKK109 pKa = 11.63AKK111 pKa = 9.55IAGTGFHH118 pKa = 7.01AGALALVRR126 pKa = 11.84LPPNVDD132 pKa = 3.14PATVSDD138 pKa = 4.13VNKK141 pKa = 7.92FTCFEE146 pKa = 4.22YY147 pKa = 10.73EE148 pKa = 4.06IIDD151 pKa = 4.06PKK153 pKa = 11.13LLEE156 pKa = 4.4CVSRR160 pKa = 11.84SVMDD164 pKa = 3.45QRR166 pKa = 11.84NTMYY170 pKa = 10.36HH171 pKa = 5.62YY172 pKa = 11.06NPYY175 pKa = 10.68DD176 pKa = 3.32ATNYY180 pKa = 10.63NSFGGNFCIFVLQALNTSSSGSTSISVQIFSKK212 pKa = 10.59ASEE215 pKa = 4.44DD216 pKa = 3.97FEE218 pKa = 4.38MLQIIPPNITTLSSTT233 pKa = 3.75

Molecular weight: 24.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KLY1|A0A1L3KLY1_9VIRU Uncharacterized protein OS=Hubei picorna-like virus 77 OX=1923161 PE=4 SV=1
MM1 pKa = 7.06FQYY4 pKa = 10.48PHH6 pKa = 7.36LVRR9 pKa = 11.84PNPSNFIKK17 pKa = 10.27IYY19 pKa = 9.87VYY21 pKa = 10.44QIVHH25 pKa = 6.52LRR27 pKa = 11.84LIMYY31 pKa = 7.61LTNRR35 pKa = 11.84YY36 pKa = 8.66AHH38 pKa = 7.23LLPHH42 pKa = 6.63PLGRR46 pKa = 11.84TPRR49 pKa = 11.84DD50 pKa = 3.45PNSVFTSRR58 pKa = 11.84DD59 pKa = 3.32LPLTPLTTNRR69 pKa = 11.84PRR71 pKa = 11.84NISHH75 pKa = 6.62INEE78 pKa = 4.42SVSSPSRR85 pKa = 11.84YY86 pKa = 9.79SSLSNHH92 pKa = 6.41GPLTHH97 pKa = 6.79SSYY100 pKa = 10.6ATASTPSIHH109 pKa = 6.49SVPSRR114 pKa = 11.84SPSSVFSRR122 pKa = 11.84ANSSSSRR129 pKa = 11.84NSSSIFSRR137 pKa = 11.84SGSQHH142 pKa = 4.48STVPHH147 pKa = 6.81RR148 pKa = 11.84LPHH151 pKa = 5.21NQIVTRR157 pKa = 11.84ATHH160 pKa = 5.99PMSTLTHH167 pKa = 6.71PLSLVPFVGSNSKK180 pKa = 10.21PRR182 pKa = 11.84VDD184 pKa = 4.72FPSHH188 pKa = 6.71KK189 pKa = 9.37YY190 pKa = 10.51SKK192 pKa = 11.05VSIQDD197 pKa = 3.32VTPTKK202 pKa = 10.62SKK204 pKa = 11.54SNFLGDD210 pKa = 3.58AMHH213 pKa = 6.97GAGSGFFAGAGLATGQIYY231 pKa = 8.08STNRR235 pKa = 11.84KK236 pKa = 8.94AKK238 pKa = 10.52AIEE241 pKa = 4.04QYY243 pKa = 10.96NADD246 pKa = 3.56KK247 pKa = 10.85VAAYY251 pKa = 7.4EE252 pKa = 4.09KK253 pKa = 10.65SGLPSYY259 pKa = 9.41MAYY262 pKa = 10.91SNTAPSPPVTATIQGNIVHH281 pKa = 6.18KK282 pKa = 9.32TGNSFQSIGNQPSSYY297 pKa = 10.38LSQASGFANMM307 pKa = 4.8

Molecular weight: 33.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2802

233

2029

700.5

78.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.103 ± 1.065

1.499 ± 0.419

6.103 ± 1.217

3.747 ± 0.855

4.996 ± 0.343

4.889 ± 0.173

3.069 ± 0.476

5.996 ± 0.319

5.318 ± 0.781

9.35 ± 0.829

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.856 ± 0.098

5.746 ± 0.329

6.067 ± 0.979

3.319 ± 0.155

4.033 ± 0.502

8.708 ± 2.744

7.423 ± 0.275

6.567 ± 0.794

0.714 ± 0.253

4.497 ± 0.346
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski