Cattle blood-associated gemycircularvirus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykibivirus; Gemykibivirus cowchi1

Average proteome isoelectric point is 7.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2L0HH91|A0A2L0HH91_9VIRU Replication-associated protein OS=Cattle blood-associated gemycircularvirus OX=2077296 PE=3 SV=1
MM1 pKa = 7.37SQFQFNHH8 pKa = 5.38IQLGLTYY15 pKa = 9.3PQARR19 pKa = 11.84GLTKK23 pKa = 10.26EE24 pKa = 3.91LLRR27 pKa = 11.84IYY29 pKa = 10.69LEE31 pKa = 3.35NWYY34 pKa = 10.08FNNGTCEE41 pKa = 3.96VRR43 pKa = 11.84VTKK46 pKa = 10.46YY47 pKa = 10.66LIAEE51 pKa = 4.34EE52 pKa = 4.04KK53 pKa = 10.83HH54 pKa = 6.26EE55 pKa = 4.74DD56 pKa = 4.0GEE58 pKa = 4.63PHH60 pKa = 5.36FHH62 pKa = 6.74VYY64 pKa = 10.6LRR66 pKa = 11.84FDD68 pKa = 3.19NKK70 pKa = 9.99LRR72 pKa = 11.84ARR74 pKa = 11.84EE75 pKa = 3.89TRR77 pKa = 11.84LFDD80 pKa = 3.76IFFEE84 pKa = 4.69STGKK88 pKa = 10.53LYY90 pKa = 10.68HH91 pKa = 6.85PYY93 pKa = 9.68IEE95 pKa = 5.0KK96 pKa = 10.28IRR98 pKa = 11.84GLRR101 pKa = 11.84NMIKK105 pKa = 10.22YY106 pKa = 7.58CTKK109 pKa = 10.72EE110 pKa = 3.88DD111 pKa = 4.03TEE113 pKa = 4.27PLANFDD119 pKa = 3.48WHH121 pKa = 6.99PSTEE125 pKa = 4.21NKK127 pKa = 10.37DD128 pKa = 3.66KK129 pKa = 11.56GIDD132 pKa = 3.23WEE134 pKa = 4.45EE135 pKa = 4.37IYY137 pKa = 10.99SVEE140 pKa = 4.18YY141 pKa = 10.07PSAQAFLDD149 pKa = 3.92TVMARR154 pKa = 11.84YY155 pKa = 8.52PSYY158 pKa = 9.96FTGHH162 pKa = 6.86YY163 pKa = 9.83IPLRR167 pKa = 11.84QLAYY171 pKa = 10.47DD172 pKa = 3.72RR173 pKa = 11.84YY174 pKa = 8.52EE175 pKa = 4.53KK176 pKa = 10.78NVEE179 pKa = 4.02EE180 pKa = 4.23YY181 pKa = 10.36RR182 pKa = 11.84PKK184 pKa = 10.67YY185 pKa = 10.81LNFEE189 pKa = 4.23NVPQILNSWVDD200 pKa = 3.64CNLFNDD206 pKa = 3.79KK207 pKa = 10.13EE208 pKa = 4.35RR209 pKa = 11.84PYY211 pKa = 10.52SLIMIGPSRR220 pKa = 11.84TGKK223 pKa = 8.12TEE225 pKa = 3.5WARR228 pKa = 11.84SIGRR232 pKa = 11.84HH233 pKa = 4.97MYY235 pKa = 9.09FCNYY239 pKa = 9.5FNLDD243 pKa = 3.38LWDD246 pKa = 3.97EE247 pKa = 4.39DD248 pKa = 3.25ALYY251 pKa = 10.92AVFDD255 pKa = 5.74DD256 pKa = 4.68MDD258 pKa = 5.5CDD260 pKa = 3.85PDD262 pKa = 4.32IPFEE266 pKa = 4.03KK267 pKa = 10.41CFRR270 pKa = 11.84SWKK273 pKa = 10.55AFFGAQKK280 pKa = 10.49EE281 pKa = 4.23FTVTDD286 pKa = 3.56KK287 pKa = 11.36YY288 pKa = 11.14KK289 pKa = 10.7RR290 pKa = 11.84KK291 pKa = 9.97QKK293 pKa = 10.55VKK295 pKa = 8.81WGKK298 pKa = 9.06PIIWISNNEE307 pKa = 4.09FNCKK311 pKa = 9.79SSTLDD316 pKa = 3.55YY317 pKa = 10.58IRR319 pKa = 11.84KK320 pKa = 9.14NSVRR324 pKa = 11.84VNVYY328 pKa = 10.35SKK330 pKa = 11.11LYY332 pKa = 10.34

Molecular weight: 40.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2L0HH91|A0A2L0HH91_9VIRU Replication-associated protein OS=Cattle blood-associated gemycircularvirus OX=2077296 PE=3 SV=1
MM1 pKa = 7.45AGSVKK6 pKa = 10.53FILPTNKK13 pKa = 8.97MPGRR17 pKa = 11.84NNRR20 pKa = 11.84QKK22 pKa = 10.83RR23 pKa = 11.84IATRR27 pKa = 11.84ATNRR31 pKa = 11.84SRR33 pKa = 11.84RR34 pKa = 11.84ITRR37 pKa = 11.84RR38 pKa = 11.84LVRR41 pKa = 11.84RR42 pKa = 11.84YY43 pKa = 10.23GPDD46 pKa = 2.62IASFMIRR53 pKa = 11.84NLANRR58 pKa = 11.84SNLTSRR64 pKa = 11.84LRR66 pKa = 11.84QRR68 pKa = 11.84LRR70 pKa = 11.84IKK72 pKa = 10.25RR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84KK76 pKa = 8.89AKK78 pKa = 9.63YY79 pKa = 8.11VSVRR83 pKa = 11.84ANVAGTTHH91 pKa = 5.81TTSNMIVRR99 pKa = 11.84KK100 pKa = 7.97TPAEE104 pKa = 3.86QKK106 pKa = 10.38FLRR109 pKa = 11.84KK110 pKa = 9.45LFKK113 pKa = 9.92DD114 pKa = 3.38TPNLVKK120 pKa = 10.45HH121 pKa = 5.66VNRR124 pKa = 11.84FAFSWVGASTCSRR137 pKa = 11.84TIWYY141 pKa = 9.79SVAHH145 pKa = 6.17LKK147 pKa = 10.34FNNVYY152 pKa = 10.42DD153 pKa = 4.1YY154 pKa = 11.2LVHH157 pKa = 7.28KK158 pKa = 10.63ISAPNQGVGSAYY170 pKa = 7.4WTTTPTDD177 pKa = 3.25NRR179 pKa = 11.84YY180 pKa = 9.63VSCNPAQFIYY190 pKa = 10.18IGKK193 pKa = 8.6CTFNYY198 pKa = 10.11EE199 pKa = 3.94IYY201 pKa = 10.91NPTNYY206 pKa = 9.89IMTVYY211 pKa = 10.13IYY213 pKa = 11.07DD214 pKa = 4.57LVCKK218 pKa = 9.98HH219 pKa = 6.22DD220 pKa = 3.84TPFEE224 pKa = 3.98ITYY227 pKa = 10.48GSQTDD232 pKa = 3.6VEE234 pKa = 4.64QISRR238 pKa = 11.84SQPEE242 pKa = 3.93CCMIQSSRR250 pKa = 11.84PLTSSAAVDD259 pKa = 3.51AEE261 pKa = 4.01KK262 pKa = 8.66WTVGDD267 pKa = 3.65TTDD270 pKa = 3.97EE271 pKa = 4.36VNNTQQGVGGPAWNAVGMKK290 pKa = 8.21PTDD293 pKa = 3.57YY294 pKa = 11.22FCFNSLWKK302 pKa = 10.8VKK304 pKa = 10.15GIKK307 pKa = 9.98KK308 pKa = 10.08IILPPASSHH317 pKa = 4.99HH318 pKa = 6.22HH319 pKa = 4.46VVVYY323 pKa = 10.38NPKK326 pKa = 10.56KK327 pKa = 10.49KK328 pKa = 8.87ITWGNLVYY336 pKa = 10.24PRR338 pKa = 11.84QDD340 pKa = 2.53ISKK343 pKa = 8.26RR344 pKa = 11.84TGKK347 pKa = 10.68YY348 pKa = 10.36GIGGLTQSTLFGFEE362 pKa = 3.97GQLGFEE368 pKa = 4.79KK369 pKa = 10.82DD370 pKa = 3.22QALDD374 pKa = 3.59NEE376 pKa = 4.89KK377 pKa = 10.97VGTLPGKK384 pKa = 10.21IVVKK388 pKa = 9.82CIRR391 pKa = 11.84KK392 pKa = 8.48INCYY396 pKa = 9.76NFPITSSTVISKK408 pKa = 10.89NNLYY412 pKa = 10.22TNLSNPEE419 pKa = 3.9IFTDD423 pKa = 3.99LTTRR427 pKa = 11.84PAEE430 pKa = 4.11TAA432 pKa = 3.28

Molecular weight: 49.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

764

332

432

382.0

44.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.843 ± 0.592

2.094 ± 0.009

4.712 ± 1.031

4.974 ± 1.826

5.105 ± 0.972

4.974 ± 0.676

2.094 ± 0.202

6.283 ± 0.358

7.592 ± 0.153

6.414 ± 0.713

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.702 ± 0.068

6.937 ± 0.391

4.843 ± 0.208

3.141 ± 0.275

6.937 ± 0.391

6.021 ± 0.768

7.461 ± 1.688

5.89 ± 1.069

2.094 ± 0.394

5.89 ± 1.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski