Apis mellifera associated microvirus 38

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UTI8|A0A3S8UTI8_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 38 OX=2494767 PE=4 SV=1
MM1 pKa = 7.54KK2 pKa = 10.37VFQNIEE8 pKa = 3.8TGEE11 pKa = 4.32IITTHH16 pKa = 6.82DD17 pKa = 3.47KK18 pKa = 10.84FGQEE22 pKa = 4.46LPDD25 pKa = 4.33PTPMAPPVGYY35 pKa = 10.23VRR37 pKa = 11.84QPSLAEE43 pKa = 3.8QIRR46 pKa = 11.84NMVRR50 pKa = 11.84SQHH53 pKa = 5.55LAMVAEE59 pKa = 4.37SQGFEE64 pKa = 4.21TFEE67 pKa = 4.03EE68 pKa = 4.81ADD70 pKa = 4.47DD71 pKa = 5.34LDD73 pKa = 5.02IPDD76 pKa = 5.31DD77 pKa = 4.32VDD79 pKa = 4.48PSSPWEE85 pKa = 4.05EE86 pKa = 4.48VYY88 pKa = 10.64EE89 pKa = 4.3PPLPPGNITPLEE101 pKa = 4.01EE102 pKa = 5.6AISQAKK108 pKa = 9.14ARR110 pKa = 11.84QPAQSPPEE118 pKa = 4.09PLSAGGASPPAPTSPPPEE136 pKa = 5.22GGAA139 pKa = 3.53

Molecular weight: 14.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UTL9|A0A3S8UTL9_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 38 OX=2494767 PE=4 SV=1
MM1 pKa = 7.7RR2 pKa = 11.84CANPYY7 pKa = 8.39TGSGGMFGCGQCLPCRR23 pKa = 11.84FNRR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84TWQHH32 pKa = 5.33RR33 pKa = 11.84LILEE37 pKa = 4.62ATQHH41 pKa = 4.38SHH43 pKa = 4.27QTFVTLTYY51 pKa = 10.76DD52 pKa = 3.74DD53 pKa = 3.76EE54 pKa = 4.75HH55 pKa = 8.37VPEE58 pKa = 4.38GHH60 pKa = 6.01TLVPKK65 pKa = 10.42HH66 pKa = 5.99LQDD69 pKa = 3.25FLKK72 pKa = 10.52RR73 pKa = 11.84LRR75 pKa = 11.84HH76 pKa = 5.12KK77 pKa = 10.16QQIRR81 pKa = 11.84YY82 pKa = 8.14FAVGEE87 pKa = 4.16YY88 pKa = 11.11GEE90 pKa = 4.57TTQRR94 pKa = 11.84PHH96 pKa = 5.1YY97 pKa = 9.83HH98 pKa = 6.58LALFGYY104 pKa = 7.63PNCNWLQSRR113 pKa = 11.84YY114 pKa = 7.48AHH116 pKa = 7.49RR117 pKa = 11.84KK118 pKa = 7.26RR119 pKa = 11.84CCVACDD125 pKa = 4.23TIRR128 pKa = 11.84DD129 pKa = 3.33TWGRR133 pKa = 11.84GNVFLGTLSSDD144 pKa = 3.45SASYY148 pKa = 9.58VAGYY152 pKa = 9.19VCKK155 pKa = 10.71KK156 pKa = 6.51MTNKK160 pKa = 10.15DD161 pKa = 3.6DD162 pKa = 3.55PRR164 pKa = 11.84LAGRR168 pKa = 11.84HH169 pKa = 5.46PEE171 pKa = 4.19FSRR174 pKa = 11.84MSLKK178 pKa = 10.62PGIGFHH184 pKa = 6.7ALAKK188 pKa = 10.08IADD191 pKa = 4.89AIRR194 pKa = 11.84LNKK197 pKa = 9.51IDD199 pKa = 4.15TLLGDD204 pKa = 3.79VPASLRR210 pKa = 11.84HH211 pKa = 5.92GSRR214 pKa = 11.84IMPLGRR220 pKa = 11.84YY221 pKa = 8.45LRR223 pKa = 11.84SQLRR227 pKa = 11.84LAVMGQKK234 pKa = 9.75NASPEE239 pKa = 3.96AQIKK243 pKa = 10.34AGEE246 pKa = 4.13ALHH249 pKa = 5.81TLRR252 pKa = 11.84ILAKK256 pKa = 9.33ALNYY260 pKa = 9.67EE261 pKa = 4.34KK262 pKa = 10.34EE263 pKa = 4.07VHH265 pKa = 5.95EE266 pKa = 5.01LIKK269 pKa = 11.0EE270 pKa = 4.3EE271 pKa = 4.47IGNTAANAAMKK282 pKa = 10.43HH283 pKa = 5.56KK284 pKa = 9.33LTNRR288 pKa = 11.84RR289 pKa = 11.84SLL291 pKa = 3.44

Molecular weight: 33.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1360

76

493

226.7

25.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.412 ± 0.23

1.103 ± 0.57

4.338 ± 0.324

5.074 ± 0.672

3.456 ± 0.395

8.382 ± 0.584

3.162 ± 0.818

4.559 ± 0.264

4.265 ± 0.756

7.868 ± 0.731

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.353 ± 0.101

4.191 ± 0.342

7.5 ± 1.58

4.559 ± 1.155

7.426 ± 0.871

6.691 ± 0.681

5.956 ± 0.458

5.147 ± 0.722

1.324 ± 0.212

3.235 ± 0.487
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski