Emiliania huxleyi virus 202

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Coccolithovirus; unclassified Coccolithovirus

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 482 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8DF26|G8DF26_9PHYC Uncharacterized protein OS=Emiliania huxleyi virus 202 OX=181211 GN=EXVG_00447 PE=4 SV=1
MM1 pKa = 8.05AIPVGAIIAAVIGFIVIVGVGVGLYY26 pKa = 9.26MIAPTARR33 pKa = 11.84TGFGDD38 pKa = 3.88GEE40 pKa = 4.62LPAGMPDD47 pKa = 3.54AAVGAPDD54 pKa = 3.64EE55 pKa = 4.68TEE57 pKa = 4.08SSDD60 pKa = 3.8GLVGDD65 pKa = 4.61TFSPASDD72 pKa = 3.84ADD74 pKa = 3.84NTDD77 pKa = 3.04VTEE80 pKa = 4.23TQIATSVSVEE90 pKa = 4.06TPDD93 pKa = 3.44NYY95 pKa = 10.94EE96 pKa = 4.72FLFDD100 pKa = 4.67DD101 pKa = 4.44CPKK104 pKa = 10.66GLSEE108 pKa = 4.3NVISTSMTIGTCADD122 pKa = 3.39EE123 pKa = 4.19CTKK126 pKa = 10.64RR127 pKa = 11.84EE128 pKa = 3.86NCGAFLITGCTTTKK142 pKa = 10.71NCGGQCTLYY151 pKa = 10.78TNNTTNIIPKK161 pKa = 9.72SAFGNGLCSTTPGDD175 pKa = 3.3MKK177 pKa = 10.95LYY179 pKa = 10.11RR180 pKa = 11.84KK181 pKa = 9.61KK182 pKa = 10.9AA183 pKa = 3.31

Molecular weight: 18.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8DFV8|G8DFV8_9PHYC Uncharacterized protein OS=Emiliania huxleyi virus 202 OX=181211 GN=EXVG_00003 PE=4 SV=1
MM1 pKa = 7.27LHH3 pKa = 7.09IDD5 pKa = 4.52LICYY9 pKa = 7.8EE10 pKa = 4.8KK11 pKa = 10.88PPVPQSRR18 pKa = 11.84YY19 pKa = 6.92LTIDD23 pKa = 2.75IGRR26 pKa = 11.84FVLFGFLRR34 pKa = 11.84YY35 pKa = 9.24HH36 pKa = 7.0KK37 pKa = 10.65RR38 pKa = 11.84RR39 pKa = 11.84DD40 pKa = 3.54LPFLSKK46 pKa = 11.11GEE48 pKa = 3.98KK49 pKa = 9.79KK50 pKa = 10.5NYY52 pKa = 8.19EE53 pKa = 3.65KK54 pKa = 10.72SLRR57 pKa = 11.84KK58 pKa = 9.83CSTARR63 pKa = 11.84FRR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84PSHH71 pKa = 6.52SNPAATRR78 pKa = 11.84EE79 pKa = 3.95NRR81 pKa = 11.84AEE83 pKa = 3.87RR84 pKa = 11.84LKK86 pKa = 10.74CARR89 pKa = 11.84PRR91 pKa = 11.84LWNGLFDD98 pKa = 4.85DD99 pKa = 4.71GGRR102 pKa = 11.84RR103 pKa = 11.84GRR105 pKa = 11.84GQVRR109 pKa = 11.84SGEE112 pKa = 4.12QNKK115 pKa = 9.18KK116 pKa = 9.4QKK118 pKa = 10.56NSEE121 pKa = 4.3KK122 pKa = 10.34INNGINTDD130 pKa = 3.14INVTVTIFDD139 pKa = 4.4VISSSDD145 pKa = 3.18KK146 pKa = 11.0

Molecular weight: 17.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

482

0

482

117186

50

3203

243.1

27.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.508 ± 0.128

1.826 ± 0.08

5.687 ± 0.086

5.236 ± 0.096

4.019 ± 0.092

5.154 ± 0.109

2.493 ± 0.083

7.275 ± 0.131

6.078 ± 0.159

7.507 ± 0.113

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.126 ± 0.078

5.517 ± 0.089

5.775 ± 0.475

3.03 ± 0.06

4.756 ± 0.11

7.438 ± 0.136

6.595 ± 0.085

6.568 ± 0.086

1.103 ± 0.04

4.309 ± 0.082
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski