Pseudomonas phage PB1 (isolate Pseudomonas aeruginosa/Scotland/Bradley/1968) (PB1) (Bacteriophage PB1)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Pbunavirus; Pseudomonas virus PB1

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B8QUA6|B8QUA6_BPPPB Uncharacterized protein OS=Pseudomonas phage PB1 (isolate Pseudomonas aeruginosa/Scotland/Bradley/1968) OX=1283339 PE=4 SV=1
MM1 pKa = 7.52SGHH4 pKa = 6.4SMFDD8 pKa = 3.3FEE10 pKa = 5.89VDD12 pKa = 4.0FNSATNSATMEE23 pKa = 4.04IDD25 pKa = 3.14PGSFYY30 pKa = 11.36VGFGFGATALSVLIYY45 pKa = 10.73VDD47 pKa = 4.22GVWVSEE53 pKa = 4.11RR54 pKa = 11.84TAWIPDD60 pKa = 3.61PKK62 pKa = 10.43EE63 pKa = 3.61ISPP66 pKa = 4.12

Molecular weight: 7.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B8QUA3|B8QUA3_BPPPB Uncharacterized protein OS=Pseudomonas phage PB1 (isolate Pseudomonas aeruginosa/Scotland/Bradley/1968) OX=1283339 PE=4 SV=1
MM1 pKa = 7.33VGPTGKK7 pKa = 10.31RR8 pKa = 11.84YY9 pKa = 7.21WLRR12 pKa = 11.84YY13 pKa = 4.89EE14 pKa = 3.81TRR16 pKa = 11.84YY17 pKa = 9.33WEE19 pKa = 4.49CLDD22 pKa = 3.64GQTLRR27 pKa = 11.84LVFVINGRR35 pKa = 11.84RR36 pKa = 11.84YY37 pKa = 8.56EE38 pKa = 3.91VNRR41 pKa = 11.84RR42 pKa = 11.84VNVDD46 pKa = 2.73ILSSRR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84FEE55 pKa = 3.79SFGRR59 pKa = 11.84VLARR63 pKa = 11.84MEE65 pKa = 3.73ISLWFAVCEE74 pKa = 4.38QEE76 pKa = 4.66GPWRR80 pKa = 11.84GSVAISDD87 pKa = 3.75TALWFHH93 pKa = 6.44LTGEE97 pKa = 5.11LIHH100 pKa = 6.77KK101 pKa = 9.36CFTRR105 pKa = 11.84RR106 pKa = 11.84PKK108 pKa = 10.74

Molecular weight: 12.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

20251

17

1035

217.8

24.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.98 ± 0.306

0.928 ± 0.098

6.266 ± 0.22

7.175 ± 0.456

4.034 ± 0.142

7.249 ± 0.282

1.694 ± 0.132

6.059 ± 0.184

5.343 ± 0.306

7.506 ± 0.229

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.499 ± 0.09

4.148 ± 0.309

4.582 ± 0.247

3.822 ± 0.173

6.222 ± 0.306

6.429 ± 0.188

5.205 ± 0.222

6.444 ± 0.253

1.536 ± 0.12

2.879 ± 0.112
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski