Altererythrobacter sp. Root672

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Erythrobacteraceae; Altererythrobacter; unclassified Altererythrobacter

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3454 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0T2QI24|A0A0T2QI24_9SPHN Pilus assembly protein TadC OS=Altererythrobacter sp. Root672 OX=1736584 GN=ASD76_10195 PE=4 SV=1
MM1 pKa = 6.77LTSVRR6 pKa = 11.84SLTAATLLVGTALAATPAFAQDD28 pKa = 3.34APAEE32 pKa = 4.2KK33 pKa = 9.64TPLVTVTGSTTLVTDD48 pKa = 3.14YY49 pKa = 10.91RR50 pKa = 11.84FRR52 pKa = 11.84GVGLSGGDD60 pKa = 3.29IAIQGGLTLNTTPGFYY76 pKa = 10.49VGTWASSLEE85 pKa = 3.98DD86 pKa = 3.36SPVYY90 pKa = 10.92GEE92 pKa = 4.5VEE94 pKa = 3.88LDD96 pKa = 3.98VFGGWTGEE104 pKa = 4.28VTDD107 pKa = 5.24GLAADD112 pKa = 3.92VGVTYY117 pKa = 10.27YY118 pKa = 11.01AYY120 pKa = 9.61PSKK123 pKa = 10.77DD124 pKa = 3.1AGAGPSDD131 pKa = 2.92VWEE134 pKa = 5.02FYY136 pKa = 11.32GKK138 pKa = 10.49LKK140 pKa = 9.16PTLGPVGLTFGLYY153 pKa = 10.06YY154 pKa = 10.91SPDD157 pKa = 3.41QDD159 pKa = 4.31SLGGGDD165 pKa = 4.38NLYY168 pKa = 10.88LSADD172 pKa = 3.47AGVGIPEE179 pKa = 4.26TPISFSAHH187 pKa = 4.54VGYY190 pKa = 9.3TDD192 pKa = 4.61GFLTLTDD199 pKa = 4.36NGKK202 pKa = 9.7AWDD205 pKa = 3.73WSVGASATVLGGLSLGLSYY224 pKa = 11.32VGVEE228 pKa = 4.42GSQINGSIDD237 pKa = 3.27GVTDD241 pKa = 3.49DD242 pKa = 4.52TVVASLGYY250 pKa = 10.91AFF252 pKa = 5.53

Molecular weight: 25.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0T2QAJ2|A0A0T2QAJ2_9SPHN Thioesterase OS=Altererythrobacter sp. Root672 OX=1736584 GN=ASD76_12050 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.89GFFARR21 pKa = 11.84KK22 pKa = 8.02ATVGGRR28 pKa = 11.84KK29 pKa = 9.11VLANRR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.54RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.81KK41 pKa = 10.45LCAA44 pKa = 3.96

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3454

0

3454

1085735

29

1783

314.3

34.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.373 ± 0.055

0.787 ± 0.013

5.793 ± 0.031

6.106 ± 0.043

3.719 ± 0.027

8.924 ± 0.045

1.909 ± 0.021

4.84 ± 0.026

3.035 ± 0.032

10.018 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.314 ± 0.017

2.718 ± 0.024

5.363 ± 0.03

3.302 ± 0.022

6.976 ± 0.038

5.436 ± 0.032

5.299 ± 0.032

7.32 ± 0.031

1.505 ± 0.019

2.265 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski