Rotavirus I

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3JKM5|A0A0E3JKM5_9REOV NSP2 OS=Rotavirus I OX=1637496 PE=4 SV=1
MM1 pKa = 7.71WFLLLLPIVSSTVVIQEE18 pKa = 3.96YY19 pKa = 10.48KK20 pKa = 10.45PVDD23 pKa = 3.41VCIFVYY29 pKa = 10.58ASSGDD34 pKa = 3.6NSSAYY39 pKa = 10.13YY40 pKa = 10.66AFHH43 pKa = 6.3TWMQRR48 pKa = 11.84LQPVLATTYY57 pKa = 9.89HH58 pKa = 5.15VCGVQAVDD66 pKa = 3.45SVDD69 pKa = 3.67VIKK72 pKa = 10.79AVAEE76 pKa = 4.43CSCLNDD82 pKa = 3.74KK83 pKa = 9.63LTDD86 pKa = 3.62VNIVYY91 pKa = 10.37SDD93 pKa = 3.93EE94 pKa = 4.43PQSTLATFIGNQNTCTKK111 pKa = 10.2LQVRR115 pKa = 11.84KK116 pKa = 9.29AAYY119 pKa = 8.15IHH121 pKa = 6.39LNKK124 pKa = 10.2SEE126 pKa = 3.93EE127 pKa = 3.75FFIYY131 pKa = 9.99GKK133 pKa = 10.24RR134 pKa = 11.84DD135 pKa = 3.2LKK137 pKa = 11.26LCFLSSSYY145 pKa = 11.12LGLDD149 pKa = 3.5CDD151 pKa = 4.59SANPTTWPTIDD162 pKa = 4.41YY163 pKa = 9.03NIQSIIHH170 pKa = 6.5EE171 pKa = 4.44KK172 pKa = 10.05PIIYY176 pKa = 9.2DD177 pKa = 3.32IPEE180 pKa = 3.38ITYY183 pKa = 10.66AVGIYY188 pKa = 10.15PEE190 pKa = 4.91ADD192 pKa = 2.77PTYY195 pKa = 8.93ICKK198 pKa = 10.27RR199 pKa = 11.84VDD201 pKa = 3.51DD202 pKa = 4.45NGINPTYY209 pKa = 10.5FFAGDD214 pKa = 4.23NSIQWGDD221 pKa = 3.35QMARR225 pKa = 11.84EE226 pKa = 4.38INWGNVWNKK235 pKa = 9.36VVSVSQALYY244 pKa = 10.42KK245 pKa = 10.5ILDD248 pKa = 3.52LFFNEE253 pKa = 4.69KK254 pKa = 9.99EE255 pKa = 4.28RR256 pKa = 11.84IEE258 pKa = 3.99QQPRR262 pKa = 11.84YY263 pKa = 9.27EE264 pKa = 3.96LL265 pKa = 3.9

Molecular weight: 30.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3M4A3|A0A0E3M4A3_9REOV NSP4 OS=Rotavirus I OX=1637496 PE=4 SV=1
MM1 pKa = 7.33GNTYY5 pKa = 10.4NVHH8 pKa = 6.43NNQQVSSNTVHH19 pKa = 7.19GSGQIHH25 pKa = 6.61SEE27 pKa = 4.05DD28 pKa = 3.58QKK30 pKa = 10.02TSSQITTIVQFSNLSLLFLIALFLFISLLFKK61 pKa = 10.6CDD63 pKa = 3.54KK64 pKa = 10.37NRR66 pKa = 11.84KK67 pKa = 6.34KK68 pKa = 10.72QKK70 pKa = 8.55WNTRR74 pKa = 11.84EE75 pKa = 4.01IIEE78 pKa = 4.19LL79 pKa = 3.69

Molecular weight: 9.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

5698

79

1162

474.8

54.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.441 ± 0.676

1.211 ± 0.274

6.546 ± 0.368

5.704 ± 0.511

4.581 ± 0.349

4.23 ± 0.317

1.983 ± 0.237

8.424 ± 0.45

6.704 ± 0.491

8.529 ± 0.333

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.861 ± 0.289

6.897 ± 0.491

3.703 ± 0.302

3.896 ± 0.315

4.756 ± 0.316

7.687 ± 0.372

6.722 ± 0.348

5.476 ± 0.312

0.86 ± 0.163

3.791 ± 0.452
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski