Papaya lethal yellowing virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Sobemovirus

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J7G6A6|J7G6A6_9VIRU N-terminal protein OS=Papaya lethal yellowing virus OX=685899 GN=p2a PE=4 SV=1
MM1 pKa = 7.15SVVIEE6 pKa = 4.57VYY8 pKa = 10.66DD9 pKa = 3.76EE10 pKa = 4.14QSVSKK15 pKa = 10.72VLIEE19 pKa = 5.22SFKK22 pKa = 10.95LWPKK26 pKa = 10.79KK27 pKa = 10.76LGDD30 pKa = 4.48CVIQLKK36 pKa = 10.46GKK38 pKa = 8.48YY39 pKa = 9.36DD40 pKa = 3.44SSAVCRR46 pKa = 11.84GEE48 pKa = 3.89ITLCCEE54 pKa = 3.26KK55 pKa = 10.46CRR57 pKa = 11.84RR58 pKa = 11.84QEE60 pKa = 3.94IISFEE65 pKa = 4.12KK66 pKa = 10.42KK67 pKa = 10.09RR68 pKa = 11.84ILIHH72 pKa = 6.92CDD74 pKa = 2.83WEE76 pKa = 4.37NKK78 pKa = 9.9LCEE81 pKa = 4.74DD82 pKa = 3.65PCIDD86 pKa = 4.28RR87 pKa = 11.84FVKK90 pKa = 10.28PDD92 pKa = 3.2EE93 pKa = 5.08GFCNWCVEE101 pKa = 4.07DD102 pKa = 4.19HH103 pKa = 6.18QCTSEE108 pKa = 4.23GCSDD112 pKa = 4.3PDD114 pKa = 4.07CVFHH118 pKa = 7.69SDD120 pKa = 3.24SLEE123 pKa = 4.33FGWSEE128 pKa = 5.28SLNKK132 pKa = 9.79WIDD135 pKa = 3.28AHH137 pKa = 5.99KK138 pKa = 10.39HH139 pKa = 4.58GFVFDD144 pKa = 3.93RR145 pKa = 11.84YY146 pKa = 8.31EE147 pKa = 4.18AEE149 pKa = 3.96WYY151 pKa = 10.32HH152 pKa = 6.89PITAEE157 pKa = 3.84ILLSRR162 pKa = 11.84SPGLFRR168 pKa = 11.84DD169 pKa = 3.7

Molecular weight: 19.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J7GE58|J7GE58_9VIRU Capsid protein OS=Papaya lethal yellowing virus OX=685899 GN=cp PE=3 SV=1
MM1 pKa = 7.42ISAGRR6 pKa = 11.84VRR8 pKa = 11.84RR9 pKa = 11.84QTIKK13 pKa = 10.36NAARR17 pKa = 11.84AAAKK21 pKa = 10.33VAVQQAQRR29 pKa = 11.84VQQVAPKK36 pKa = 10.54GMNAKK41 pKa = 8.8QRR43 pKa = 11.84RR44 pKa = 11.84ALRR47 pKa = 11.84RR48 pKa = 11.84KK49 pKa = 8.29MLQSTASVQTRR60 pKa = 11.84QVTAPTAKK68 pKa = 9.38SVQVIRR74 pKa = 11.84AMPTLRR80 pKa = 11.84SRR82 pKa = 11.84NSVTGITHH90 pKa = 5.89MEE92 pKa = 3.94YY93 pKa = 10.32NQPVSVKK100 pKa = 8.83ATPTVTVDD108 pKa = 2.48NCMPYY113 pKa = 9.8TVGTWLRR120 pKa = 11.84GVASNWSKK128 pKa = 10.1WRR130 pKa = 11.84WRR132 pKa = 11.84KK133 pKa = 9.49LRR135 pKa = 11.84FYY137 pKa = 10.23YY138 pKa = 10.54LPSCATTTPGSVHH151 pKa = 6.27MGLTYY156 pKa = 10.52DD157 pKa = 4.26QYY159 pKa = 11.85DD160 pKa = 4.07TVPTNATEE168 pKa = 3.95MCNLQGYY175 pKa = 5.73TTGPVWSGAEE185 pKa = 3.84ASTALQNANVMCPPGAICCEE205 pKa = 3.73VDD207 pKa = 3.23VTRR210 pKa = 11.84FTQAWYY216 pKa = 10.13NYY218 pKa = 6.87ITSVSFTSATTVNVAIGNMYY238 pKa = 10.26SPVRR242 pKa = 11.84LVCLTDD248 pKa = 3.68EE249 pKa = 5.06GPATAVNAGKK259 pKa = 10.15ILVQYY264 pKa = 9.39EE265 pKa = 4.04IEE267 pKa = 4.61LIEE270 pKa = 4.45PVTSALNLL278 pKa = 3.6

Molecular weight: 30.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2009

169

1001

502.3

55.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.869 ± 1.042

3.285 ± 0.772

4.231 ± 0.751

6.969 ± 1.02

2.588 ± 0.558

7.566 ± 0.911

1.941 ± 0.34

4.729 ± 0.415

5.724 ± 0.626

8.86 ± 0.935

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.19 ± 0.353

4.032 ± 0.422

4.629 ± 0.417

2.787 ± 0.72

5.077 ± 0.501

8.213 ± 0.381

5.923 ± 1.272

8.213 ± 0.843

2.439 ± 0.227

3.733 ± 0.379
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski