Lactococcus phage 936 group phage PhiA1127

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Skunavirus; unclassified Skunavirus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126H9M9|A0A126H9M9_9CAUD Protease OS=Lactococcus phage 936 group phage PhiA1127 OX=1636572 GN=PhiA1127_06 PE=4 SV=1
MM1 pKa = 7.74SEE3 pKa = 3.85FDD5 pKa = 6.09SYY7 pKa = 11.5IDD9 pKa = 3.33WYY11 pKa = 11.58NNLLTMPLNDD21 pKa = 3.86VILGVKK27 pKa = 9.05DD28 pKa = 3.82TIQDD32 pKa = 3.37KK33 pKa = 8.92TVYY36 pKa = 10.59LSLSDD41 pKa = 3.95SKK43 pKa = 10.99VLKK46 pKa = 9.75MDD48 pKa = 3.09NTSFVMGYY56 pKa = 9.13YY57 pKa = 8.74YY58 pKa = 10.45QVVLSVKK65 pKa = 10.52DD66 pKa = 3.48VDD68 pKa = 4.31DD69 pKa = 4.13EE70 pKa = 4.3LVRR73 pKa = 11.84LVGDD77 pKa = 3.57VLRR80 pKa = 11.84DD81 pKa = 3.4GWNMTNWSEE90 pKa = 4.32NSHH93 pKa = 6.61LYY95 pKa = 10.68NYY97 pKa = 8.05TGSVYY102 pKa = 10.35LPCGAGGQPWQQ113 pKa = 3.69

Molecular weight: 12.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126H9R7|A0A126H9R7_9CAUD Uncharacterized protein OS=Lactococcus phage 936 group phage PhiA1127 OX=1636572 GN=PhiA1127_32 PE=4 SV=1
MM1 pKa = 7.68NNMCKK6 pKa = 9.79KK7 pKa = 10.35RR8 pKa = 11.84KK9 pKa = 5.77YY10 pKa = 8.39TKK12 pKa = 9.78MGALYY17 pKa = 10.41SIVSAQHH24 pKa = 5.58AKK26 pKa = 10.24KK27 pKa = 10.44SKK29 pKa = 10.17KK30 pKa = 9.93NKK32 pKa = 8.77NDD34 pKa = 4.1KK35 pKa = 10.66IPVRR39 pKa = 11.84AYY41 pKa = 8.84YY42 pKa = 10.05CKK44 pKa = 9.82WCNLYY49 pKa = 10.42HH50 pKa = 7.32LSSQQRR56 pKa = 11.84LNIKK60 pKa = 9.21TGVIGG65 pKa = 3.97

Molecular weight: 7.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

8706

26

916

167.4

19.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.478 ± 0.628

0.609 ± 0.147

5.594 ± 0.288

7.558 ± 0.587

4.124 ± 0.361

6.076 ± 0.636

1.229 ± 0.189

6.926 ± 0.33

9.028 ± 0.48

8.615 ± 0.314

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.493 ± 0.175

6.915 ± 0.292

2.32 ± 0.258

3.756 ± 0.228

3.618 ± 0.296

6.191 ± 0.552

6.559 ± 0.379

6.317 ± 0.314

1.344 ± 0.12

4.25 ± 0.459
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski