Bacillus phage vB_BveM-Goe7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; unclassified Bastillevirinae

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 240 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A516KN69|A0A516KN69_9CAUD Uncharacterized protein OS=Bacillus phage vB_BveM-Goe7 OX=2593639 GN=Goe7_c00190 PE=4 SV=1
MM1 pKa = 6.93TRR3 pKa = 11.84DD4 pKa = 3.52MQEE7 pKa = 3.67LTDD10 pKa = 4.35RR11 pKa = 11.84LEE13 pKa = 5.07DD14 pKa = 3.46MQTTYY19 pKa = 11.88NDD21 pKa = 2.94MALQYY26 pKa = 10.02EE27 pKa = 4.5EE28 pKa = 4.64TGAEE32 pKa = 4.05YY33 pKa = 11.01LLDD36 pKa = 5.16DD37 pKa = 3.96MTEE40 pKa = 4.08LEE42 pKa = 4.62LDD44 pKa = 3.4IEE46 pKa = 4.26EE47 pKa = 4.23LQIEE51 pKa = 4.25IEE53 pKa = 4.15IMQEE57 pKa = 3.75YY58 pKa = 10.35APYY61 pKa = 10.64EE62 pKa = 4.28HH63 pKa = 6.59MTGIQRR69 pKa = 11.84DD70 pKa = 3.71MKK72 pKa = 11.28LNGLSQADD80 pKa = 3.85FVV82 pKa = 4.22

Molecular weight: 9.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A516KND8|A0A516KND8_9CAUD Uncharacterized protein OS=Bacillus phage vB_BveM-Goe7 OX=2593639 GN=Goe7_c00890 PE=4 SV=1
MM1 pKa = 7.49PRR3 pKa = 11.84KK4 pKa = 9.96KK5 pKa = 10.21SVRR8 pKa = 11.84PKK10 pKa = 10.46LFKK13 pKa = 11.01SNSATKK19 pKa = 9.51STMKK23 pKa = 10.68RR24 pKa = 11.84LGDD27 pKa = 3.54TLVQKK32 pKa = 9.45TLDD35 pKa = 3.5AGMDD39 pKa = 3.68AARR42 pKa = 11.84DD43 pKa = 3.84ALPSDD48 pKa = 3.81TNVVRR53 pKa = 11.84KK54 pKa = 9.07PKK56 pKa = 10.01YY57 pKa = 9.58LQVTEE62 pKa = 3.98KK63 pKa = 10.69RR64 pKa = 11.84MNKK67 pKa = 10.15LGIIDD72 pKa = 4.31LKK74 pKa = 11.04PYY76 pKa = 9.97FKK78 pKa = 10.69KK79 pKa = 10.71SPKK82 pKa = 8.81TKK84 pKa = 8.87RR85 pKa = 11.84TKK87 pKa = 10.41SGGWYY92 pKa = 8.21LTVPISIKK100 pKa = 10.46KK101 pKa = 9.49KK102 pKa = 9.11DD103 pKa = 3.56MSRR106 pKa = 11.84RR107 pKa = 11.84MYY109 pKa = 10.67DD110 pKa = 2.97QLRR113 pKa = 11.84LEE115 pKa = 5.06RR116 pKa = 11.84IAPSTQKK123 pKa = 9.5TVISDD128 pKa = 3.37YY129 pKa = 10.98LYY131 pKa = 10.55DD132 pKa = 3.6RR133 pKa = 11.84RR134 pKa = 11.84RR135 pKa = 11.84TSEE138 pKa = 4.03SSALNYY144 pKa = 9.67KK145 pKa = 9.94PKK147 pKa = 10.71SNNVTKK153 pKa = 10.36TRR155 pKa = 11.84TGKK158 pKa = 9.49NRR160 pKa = 11.84HH161 pKa = 6.09SYY163 pKa = 8.22VAYY166 pKa = 8.91RR167 pKa = 11.84TVTDD171 pKa = 4.35KK172 pKa = 11.48SPASSWVINRR182 pKa = 11.84GKK184 pKa = 10.51VNKK187 pKa = 9.44DD188 pKa = 3.06DD189 pKa = 3.86HH190 pKa = 7.22SKK192 pKa = 9.39TFVKK196 pKa = 10.71NVDD199 pKa = 3.82RR200 pKa = 11.84LMKK203 pKa = 9.95WKK205 pKa = 9.36MKK207 pKa = 10.66NGWSS211 pKa = 3.45

Molecular weight: 24.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

240

0

240

45867

38

1270

191.1

21.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.192 ± 0.184

0.798 ± 0.072

6.639 ± 0.168

8.2 ± 0.332

3.804 ± 0.106

6.543 ± 0.273

1.81 ± 0.095

6.142 ± 0.116

7.467 ± 0.188

8.427 ± 0.184

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.549 ± 0.105

5.008 ± 0.148

3.17 ± 0.158

3.591 ± 0.147

4.646 ± 0.125

6.514 ± 0.209

6.089 ± 0.183

7.101 ± 0.158

0.999 ± 0.058

4.312 ± 0.131
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski