Listeria phage A006

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8ATX0|A8ATX0_9CAUD Gp26 OS=Listeria phage A006 OX=330399 PE=4 SV=1
MM1 pKa = 7.69GKK3 pKa = 8.18TYY5 pKa = 9.62WYY7 pKa = 11.1NEE9 pKa = 3.99GTDD12 pKa = 3.35TLLTEE17 pKa = 4.29KK18 pKa = 10.28EE19 pKa = 4.32YY20 pKa = 11.27KK21 pKa = 10.35EE22 pKa = 4.0LMEE25 pKa = 5.0RR26 pKa = 11.84EE27 pKa = 4.08AKK29 pKa = 10.29ALYY32 pKa = 10.53EE33 pKa = 4.01EE34 pKa = 4.52VQEE37 pKa = 4.16EE38 pKa = 4.33EE39 pKa = 4.39KK40 pKa = 11.12DD41 pKa = 3.76FEE43 pKa = 4.59SSEE46 pKa = 4.05KK47 pKa = 10.25TSFEE51 pKa = 4.18EE52 pKa = 4.55FLKK55 pKa = 9.96TCYY58 pKa = 10.08EE59 pKa = 4.11NEE61 pKa = 4.1SDD63 pKa = 6.08FVLSDD68 pKa = 3.41NEE70 pKa = 4.34GNKK73 pKa = 10.27LEE75 pKa = 4.22EE76 pKa = 4.18WW77 pKa = 3.68

Molecular weight: 9.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8ATZ9|A8ATZ9_9CAUD Gp55 OS=Listeria phage A006 OX=330399 PE=4 SV=1
MM1 pKa = 7.32SRR3 pKa = 11.84KK4 pKa = 8.36EE5 pKa = 3.77LRR7 pKa = 11.84KK8 pKa = 9.96KK9 pKa = 8.16QWEE12 pKa = 4.66VITMIEE18 pKa = 4.01KK19 pKa = 10.72SKK21 pKa = 9.57TLTDD25 pKa = 3.39RR26 pKa = 11.84KK27 pKa = 10.64NLIKK31 pKa = 10.65KK32 pKa = 10.46LEE34 pKa = 4.07TLEE37 pKa = 4.34ARR39 pKa = 11.84GDD41 pKa = 3.85KK42 pKa = 10.58EE43 pKa = 4.63KK44 pKa = 11.31GLATPTQLLSIFTVTEE60 pKa = 3.54YY61 pKa = 10.93RR62 pKa = 11.84RR63 pKa = 11.84LSKK66 pKa = 10.83KK67 pKa = 9.68LTDD70 pKa = 3.48TEE72 pKa = 4.02IAEE75 pKa = 4.37DD76 pKa = 3.58MGISRR81 pKa = 11.84SALIEE86 pKa = 4.18FKK88 pKa = 10.59RR89 pKa = 11.84KK90 pKa = 9.24NGLSIRR96 pKa = 11.84QKK98 pKa = 10.54VATT101 pKa = 4.36

Molecular weight: 11.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

11849

29

1599

191.1

21.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.785 ± 0.584

0.675 ± 0.127

6.102 ± 0.22

8.144 ± 0.599

4.051 ± 0.305

5.773 ± 0.344

1.468 ± 0.182

7.14 ± 0.326

9.241 ± 0.447

8.136 ± 0.297

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.658 ± 0.163

6.583 ± 0.262

2.642 ± 0.212

3.612 ± 0.218

3.781 ± 0.281

6.254 ± 0.319

6.076 ± 0.308

5.823 ± 0.252

1.198 ± 0.156

3.857 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski