Vagococcus lutrae LBD1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Vagococcus; Vagococcus lutrae

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1736 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V6Q2J4|V6Q2J4_9ENTE UPF0145 protein T233_01479 OS=Vagococcus lutrae LBD1 OX=1408226 GN=T233_01479 PE=3 SV=1
MM1 pKa = 7.52ALVKK5 pKa = 10.05IVYY8 pKa = 10.32ASLTGNTEE16 pKa = 3.93EE17 pKa = 4.62IADD20 pKa = 3.64IVAEE24 pKa = 4.02AFEE27 pKa = 4.67NEE29 pKa = 4.19GLDD32 pKa = 4.2VEE34 pKa = 5.21INEE37 pKa = 4.46CTQVDD42 pKa = 3.64AEE44 pKa = 5.03DD45 pKa = 3.96FLEE48 pKa = 5.79ADD50 pKa = 3.26ICVVGTYY57 pKa = 9.64TYY59 pKa = 11.21DD60 pKa = 3.27EE61 pKa = 4.82GALPDD66 pKa = 5.47DD67 pKa = 3.77IVDD70 pKa = 4.75FYY72 pKa = 11.88EE73 pKa = 4.71DD74 pKa = 4.43LKK76 pKa = 11.48EE77 pKa = 4.3LDD79 pKa = 4.13LTGKK83 pKa = 10.62LYY85 pKa = 11.07GVVGSGDD92 pKa = 3.09TFYY95 pKa = 11.36EE96 pKa = 4.49MFCSCVDD103 pKa = 3.26DD104 pKa = 4.26FDD106 pKa = 5.87RR107 pKa = 11.84VFSQTGATKK116 pKa = 9.99GAEE119 pKa = 4.2SVKK122 pKa = 10.29VDD124 pKa = 4.1LAAEE128 pKa = 4.17EE129 pKa = 4.09EE130 pKa = 4.68DD131 pKa = 3.65IVRR134 pKa = 11.84LEE136 pKa = 4.07AFASEE141 pKa = 4.26LASKK145 pKa = 8.61ITEE148 pKa = 3.93

Molecular weight: 16.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V6Q340|V6Q340_9ENTE 50S ribosomal protein L9 OS=Vagococcus lutrae LBD1 OX=1408226 GN=rplI PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.14QPSKK9 pKa = 9.19RR10 pKa = 11.84KK11 pKa = 9.13HH12 pKa = 5.47KK13 pKa = 9.91RR14 pKa = 11.84VHH16 pKa = 6.02GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLANRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.66GRR39 pKa = 11.84KK40 pKa = 8.71VLSSS44 pKa = 3.31

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1736

0

1736

550350

30

3284

317.0

35.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.154 ± 0.06

0.58 ± 0.015

5.358 ± 0.046

7.709 ± 0.071

4.286 ± 0.047

6.522 ± 0.058

2.16 ± 0.03

7.393 ± 0.056

6.464 ± 0.053

9.959 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.913 ± 0.033

4.129 ± 0.045

3.552 ± 0.029

4.239 ± 0.042

4.123 ± 0.046

5.708 ± 0.04

5.982 ± 0.037

7.157 ± 0.057

0.897 ± 0.02

3.7 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski