Lake Sarah-associated circular virus-18

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126GA83|A0A126GA83_9VIRU Coat protein OS=Lake Sarah-associated circular virus-18 OX=1685744 PE=4 SV=1
MM1 pKa = 7.46TSLLPTDD8 pKa = 3.91SKK10 pKa = 8.98STRR13 pKa = 11.84WAFTAFEE20 pKa = 4.08SQYY23 pKa = 10.77TLFNEE28 pKa = 4.28MPAFIAQWGWQQEE41 pKa = 4.27VSPTTGKK48 pKa = 9.22PHH50 pKa = 5.4FQGYY54 pKa = 8.55FRR56 pKa = 11.84TTTQVRR62 pKa = 11.84FAYY65 pKa = 10.32LKK67 pKa = 8.74RR68 pKa = 11.84QFPGVHH74 pKa = 6.54LEE76 pKa = 3.9IAKK79 pKa = 10.26DD80 pKa = 3.32WEE82 pKa = 4.41SLKK85 pKa = 10.71KK86 pKa = 10.32YY87 pKa = 10.0CNKK90 pKa = 10.4AEE92 pKa = 4.05TAIPGTQVQATSNFMNMYY110 pKa = 10.46DD111 pKa = 3.93YY112 pKa = 11.19LPWILLQTIDD122 pKa = 3.31TYY124 pKa = 11.45GWQEE128 pKa = 3.63LWDD131 pKa = 4.32DD132 pKa = 3.6SRR134 pKa = 11.84EE135 pKa = 3.97EE136 pKa = 4.12YY137 pKa = 10.06IEE139 pKa = 3.88KK140 pKa = 10.53VMYY143 pKa = 7.85TARR146 pKa = 11.84AHH148 pKa = 5.91IASGNTWASWIIQNPNFKK166 pKa = 10.69QNLKK170 pKa = 10.0EE171 pKa = 3.91NGRR174 pKa = 11.84AILLGFKK181 pKa = 10.02HH182 pKa = 6.08RR183 pKa = 11.84QTDD186 pKa = 3.49RR187 pKa = 11.84QTEE190 pKa = 3.99

Molecular weight: 22.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126GA83|A0A126GA83_9VIRU Coat protein OS=Lake Sarah-associated circular virus-18 OX=1685744 PE=4 SV=1
MM1 pKa = 7.65PRR3 pKa = 11.84RR4 pKa = 11.84SNRR7 pKa = 11.84LASKK11 pKa = 10.23PRR13 pKa = 11.84LDD15 pKa = 3.41YY16 pKa = 10.95KK17 pKa = 10.71KK18 pKa = 10.29YY19 pKa = 7.86HH20 pKa = 4.79TTGIIVEE27 pKa = 4.25KK28 pKa = 10.55GKK30 pKa = 8.37TRR32 pKa = 11.84AKK34 pKa = 9.88RR35 pKa = 11.84VQSARR40 pKa = 11.84ATGALATAIKK50 pKa = 10.34KK51 pKa = 8.11VVRR54 pKa = 11.84GQAEE58 pKa = 4.28TKK60 pKa = 9.06MATWYY65 pKa = 10.86SGGTNPLGVGARR77 pKa = 11.84SNWAAEE83 pKa = 3.85PHH85 pKa = 5.85NAQITSNTTDD95 pKa = 2.71IMRR98 pKa = 11.84LIPVVAVGTGDD109 pKa = 3.32NQRR112 pKa = 11.84IGEE115 pKa = 4.5RR116 pKa = 11.84ISPSSFIVNGVVSINLSNVLVNVAPLDD143 pKa = 3.69IVAVIYY149 pKa = 9.7VLQHH153 pKa = 6.57KK154 pKa = 8.49SLKK157 pKa = 9.63TYY159 pKa = 11.07NSLQTVTQPGPPIVQSGNDD178 pKa = 3.63FSQLLLTGEE187 pKa = 4.43GDD189 pKa = 3.69TVGFDD194 pKa = 3.04GHH196 pKa = 7.03PYY198 pKa = 10.09DD199 pKa = 5.86ASLQVADD206 pKa = 5.06QYY208 pKa = 12.18YY209 pKa = 10.36KK210 pKa = 11.06LCAKK214 pKa = 10.32KK215 pKa = 10.54LIPLRR220 pKa = 11.84YY221 pKa = 9.0AGSNVVPTGSPIPGGAGVVSVANSHH246 pKa = 6.05TYY248 pKa = 8.15SARR251 pKa = 11.84YY252 pKa = 6.87TFNLKK257 pKa = 9.91KK258 pKa = 10.12HH259 pKa = 5.83IPSVLKK265 pKa = 9.58YY266 pKa = 10.17QEE268 pKa = 4.35SAVTTGQPGDD278 pKa = 4.21PLNSSIFMCVGYY290 pKa = 10.57YY291 pKa = 10.48RR292 pKa = 11.84LDD294 pKa = 3.38QTVPGAAAFLSNSYY308 pKa = 10.6VATLKK313 pKa = 10.99YY314 pKa = 10.8KK315 pKa = 10.96DD316 pKa = 3.41MM317 pKa = 5.4

Molecular weight: 34.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

507

190

317

253.5

28.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.284 ± 0.552

0.592 ± 0.039

3.748 ± 0.038

3.55 ± 1.666

3.353 ± 1.468

7.101 ± 1.424

1.972 ± 0.08

5.325 ± 0.038

5.917 ± 0.077

7.298 ± 0.275

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.972 ± 0.397

5.128 ± 0.236

5.325 ± 0.672

5.72 ± 1.31

4.734 ± 0.002

7.298 ± 1.226

8.481 ± 0.915

7.101 ± 2.693

2.17 ± 1.547

4.931 ± 0.117
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski