Hubei tetragnatha maxillosa virus 6

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEQ2|A0A1L3KEQ2_9VIRU RNA-directed RNA polymerase OS=Hubei tetragnatha maxillosa virus 6 OX=1923248 PE=4 SV=1
MM1 pKa = 7.3TSSPGYY7 pKa = 9.29PYY9 pKa = 10.03IYY11 pKa = 9.84HH12 pKa = 6.24HH13 pKa = 6.92TNNASFFHH21 pKa = 6.42YY22 pKa = 10.12KK23 pKa = 10.57DD24 pKa = 3.56GVVDD28 pKa = 4.17PDD30 pKa = 3.33RR31 pKa = 11.84LKK33 pKa = 11.11VVWEE37 pKa = 3.82MVINQIQEE45 pKa = 3.95RR46 pKa = 11.84RR47 pKa = 11.84SDD49 pKa = 4.1PIRR52 pKa = 11.84IFVKK56 pKa = 10.43PEE58 pKa = 3.16PHH60 pKa = 6.69SYY62 pKa = 10.78KK63 pKa = 10.44KK64 pKa = 10.4LQSGRR69 pKa = 11.84YY70 pKa = 8.26RR71 pKa = 11.84LISSVSIIDD80 pKa = 3.78QIIDD84 pKa = 3.08AMLFRR89 pKa = 11.84EE90 pKa = 4.81MNAQMIAQWPLLPTRR105 pKa = 11.84VGWSPYY111 pKa = 8.19YY112 pKa = 10.55GGWKK116 pKa = 8.5MMPYY120 pKa = 9.96VRR122 pKa = 11.84EE123 pKa = 3.51QMAIDD128 pKa = 3.73KK129 pKa = 10.71SSWDD133 pKa = 3.24WTVQPWIVEE142 pKa = 3.84AVLQIRR148 pKa = 11.84TEE150 pKa = 3.92MCANLTPQWEE160 pKa = 4.25EE161 pKa = 3.58LARR164 pKa = 11.84WRR166 pKa = 11.84YY167 pKa = 9.29KK168 pKa = 10.41EE169 pKa = 5.17LYY171 pKa = 9.99GSPLFVTTRR180 pKa = 11.84GSIFKK185 pKa = 9.91QQTPGVMKK193 pKa = 10.31SGCYY197 pKa = 7.69NTLMDD202 pKa = 4.32NSIMQLILHH211 pKa = 6.64ALVTQKK217 pKa = 11.29LNIPFGQIIAMGDD230 pKa = 3.46DD231 pKa = 3.85TLQDD235 pKa = 4.05ALPEE239 pKa = 5.04PILTDD244 pKa = 3.62YY245 pKa = 11.39LDD247 pKa = 3.8QLSMYY252 pKa = 10.07CNVKK256 pKa = 9.96LCEE259 pKa = 4.34KK260 pKa = 10.31KK261 pKa = 10.35RR262 pKa = 11.84EE263 pKa = 3.92FCGFNFNVNGVVEE276 pKa = 4.45PIYY279 pKa = 10.23RR280 pKa = 11.84GKK282 pKa = 9.81HH283 pKa = 4.67AYY285 pKa = 9.43KK286 pKa = 9.09MLHH289 pKa = 5.6MPIDD293 pKa = 4.01IMQSMADD300 pKa = 4.13SYY302 pKa = 11.63TMMYY306 pKa = 9.56HH307 pKa = 7.1KK308 pKa = 10.39SRR310 pKa = 11.84YY311 pKa = 9.22SPLIDD316 pKa = 3.15RR317 pKa = 11.84FFRR320 pKa = 11.84KK321 pKa = 9.57IGCEE325 pKa = 3.83VSPRR329 pKa = 11.84PYY331 pKa = 10.21RR332 pKa = 11.84DD333 pKa = 2.94LVYY336 pKa = 11.05GG337 pKa = 3.92

Molecular weight: 39.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEN8|A0A1L3KEN8_9VIRU C2H2-type domain-containing protein OS=Hubei tetragnatha maxillosa virus 6 OX=1923248 PE=4 SV=1
MM1 pKa = 7.58SSNKK5 pKa = 9.13TFVCPRR11 pKa = 11.84DD12 pKa = 3.3SRR14 pKa = 11.84KK15 pKa = 9.87FKK17 pKa = 10.29TRR19 pKa = 11.84AALQQHH25 pKa = 6.19MAASHH30 pKa = 5.19PQGRR34 pKa = 11.84PSPAPKK40 pKa = 10.14APRR43 pKa = 11.84RR44 pKa = 11.84QGNNTRR50 pKa = 11.84VPIVVPGSNGAFVRR64 pKa = 11.84FRR66 pKa = 11.84NKK68 pKa = 10.12EE69 pKa = 3.85FLKK72 pKa = 10.68EE73 pKa = 3.74LSFPTKK79 pKa = 9.68GTTLHH84 pKa = 6.24VGVHH88 pKa = 5.72FNIAGDD94 pKa = 3.86APILKK99 pKa = 9.75KK100 pKa = 10.19MSSIYY105 pKa = 10.33DD106 pKa = 3.35SYY108 pKa = 10.96RR109 pKa = 11.84IHH111 pKa = 6.02GVKK114 pKa = 10.3YY115 pKa = 10.11HH116 pKa = 5.92FTTSVSKK123 pKa = 7.86TTSGLVAMAVDD134 pKa = 4.34PGVTKK139 pKa = 11.12YY140 pKa = 10.68PGDD143 pKa = 3.65LKK145 pKa = 10.85ATLSANPHH153 pKa = 4.33VTGPIHH159 pKa = 7.58SDD161 pKa = 2.78RR162 pKa = 11.84LSITVPTVWCNPMLLRR178 pKa = 11.84EE179 pKa = 4.34VGSSNATPFQLIASVKK195 pKa = 9.31MSADD199 pKa = 3.39QPSNTIVGYY208 pKa = 10.13IEE210 pKa = 3.91IEE212 pKa = 3.81YY213 pKa = 10.31DD214 pKa = 3.21IEE216 pKa = 4.97LSGLTPXAEE225 pKa = 3.98QAGGVVAEE233 pKa = 4.07QHH235 pKa = 6.08NFTEE239 pKa = 4.82GAAARR244 pKa = 11.84IEE246 pKa = 4.12YY247 pKa = 9.98RR248 pKa = 11.84SQAKK252 pKa = 9.27EE253 pKa = 3.63PNYY256 pKa = 10.14YY257 pKa = 10.78ASNLLTNMPTAQYY270 pKa = 11.15LKK272 pKa = 11.24GEE274 pKa = 4.32LDD276 pKa = 3.49YY277 pKa = 11.66DD278 pKa = 4.03FTKK281 pKa = 10.05STTPPSVLGPVEE293 pKa = 4.78EE294 pKa = 4.62INSTGIPQWNVDD306 pKa = 4.24SYY308 pKa = 11.51PHH310 pKa = 6.42TNLTEE315 pKa = 4.97DD316 pKa = 3.52INVQTRR322 pKa = 11.84IVGSMQMTGILFVNWVSFIDD342 pKa = 4.33FLGLDD347 pKa = 3.66VEE349 pKa = 4.69STKK352 pKa = 11.0GFILTASSTVCDD364 pKa = 4.34VIFNAQPKK372 pKa = 9.38IVWNAAKK379 pKa = 8.54STCYY383 pKa = 10.59ASVGMWLKK391 pKa = 9.83TKK393 pKa = 10.21TEE395 pKa = 3.97APVFPVLTLEE405 pKa = 4.79LDD407 pKa = 4.89FIGHH411 pKa = 5.8RR412 pKa = 11.84HH413 pKa = 5.45SGEE416 pKa = 3.61NAIFFVYY423 pKa = 9.57HH424 pKa = 5.84HH425 pKa = 6.76VSMAFSKK432 pKa = 10.71LKK434 pKa = 10.23SLPWSMDD441 pKa = 2.82YY442 pKa = 10.87DD443 pKa = 3.99VPRR446 pKa = 11.84KK447 pKa = 8.56PKK449 pKa = 7.98YY450 pKa = 9.0WFVRR454 pKa = 11.84KK455 pKa = 8.47YY456 pKa = 7.58WRR458 pKa = 11.84KK459 pKa = 9.2FKK461 pKa = 10.6TSAKK465 pKa = 10.16RR466 pKa = 11.84NN467 pKa = 3.61

Molecular weight: 51.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1405

337

601

468.3

53.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.623 ± 0.762

1.352 ± 0.214

4.27 ± 0.352

5.836 ± 1.075

4.057 ± 0.357

5.907 ± 0.293

3.06 ± 0.255

6.263 ± 0.563

6.69 ± 0.535

7.046 ± 0.257

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.915 ± 0.735

3.488 ± 0.65

6.121 ± 0.403

3.701 ± 0.544

5.409 ± 0.463

7.544 ± 0.528

6.762 ± 0.868

6.975 ± 0.481

1.566 ± 0.421

4.342 ± 0.621
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski