Azospirillum sp. TSO22-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Azospirillaceae; Azospirillum; unclassified Azospirillum

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6297 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U1W0Q5|A0A2U1W0Q5_9PROT Glycosyl transferase OS=Azospirillum sp. TSO22-1 OX=716789 GN=TSO221_19525 PE=4 SV=1
DDD2 pKa = 4.58LTGGTGNDDD11 pKa = 3.31YYY13 pKa = 11.34VDDD16 pKa = 3.82TGVTVVEEE24 pKa = 4.49AGGGMDDD31 pKa = 3.55EE32 pKa = 4.74RR33 pKa = 11.84TSLASYYY40 pKa = 8.79LAAEEE45 pKa = 4.36EEE47 pKa = 4.47YY48 pKa = 9.46TYYY51 pKa = 10.23GSGNFTGNGNTLANLIQGGAGNDDD75 pKa = 3.54LTGGGGSDDD84 pKa = 3.74LTGAAGADDD93 pKa = 3.11FKKK96 pKa = 11.13GTADDD101 pKa = 3.67VTGANADDD109 pKa = 4.42ITDDD113 pKa = 4.24VAGTDDD119 pKa = 3.74IDDD122 pKa = 3.62SGVDDD127 pKa = 3.34NSAVSGDDD135 pKa = 3.55LYYY138 pKa = 11.29FIGTSAFSGTAGQLRR153 pKa = 11.84YYY155 pKa = 9.79VLGDDD160 pKa = 4.03TTHHH164 pKa = 8.07YYY166 pKa = 10.86DDD168 pKa = 4.02HH169 pKa = 6.76GDDD172 pKa = 3.33NADDD176 pKa = 3.66EEE178 pKa = 4.72VLTGSLSLTAANFKKK193 pKa = 10.48

Molecular weight: 19.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U1W0S5|A0A2U1W0S5_9PROT 50S ribosomal protein L24 OS=Azospirillum sp. TSO22-1 OX=716789 GN=rplX PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.84LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.01VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.91VLSAA44 pKa = 4.11

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6297

0

6297

1943342

25

2863

308.6

33.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.506 ± 0.047

0.885 ± 0.011

5.606 ± 0.023

5.654 ± 0.028

3.411 ± 0.018

8.92 ± 0.033

2.126 ± 0.017

4.133 ± 0.023

2.803 ± 0.027

10.668 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.321 ± 0.014

2.241 ± 0.018

5.614 ± 0.027

2.875 ± 0.017

7.766 ± 0.034

4.536 ± 0.024

5.326 ± 0.027

8.19 ± 0.027

1.341 ± 0.012

2.079 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski