Mycobacterium phage Nyxis

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W0LJ83|W0LJ83_9CAUD Uncharacterized protein OS=Mycobacterium phage Nyxis OX=1445714 GN=42 PE=4 SV=1
MM1 pKa = 7.4KK2 pKa = 10.52LVTALLLLAVVLGLTACEE20 pKa = 4.21GDD22 pKa = 3.92SGGSDD27 pKa = 3.46YY28 pKa = 11.41DD29 pKa = 3.77GPNGVIFMPVQGNPVGIPIFFF50 pKa = 4.82

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W0LNF3|W0LNF3_9CAUD Uncharacterized protein OS=Mycobacterium phage Nyxis OX=1445714 GN=51 PE=4 SV=1
MM1 pKa = 7.74RR2 pKa = 11.84KK3 pKa = 9.38KK4 pKa = 9.93EE5 pKa = 4.04LKK7 pKa = 9.85RR8 pKa = 11.84ALRR11 pKa = 11.84EE12 pKa = 4.06ANLSIDD18 pKa = 3.98ALASSNTTLWEE29 pKa = 3.82QRR31 pKa = 11.84EE32 pKa = 3.98EE33 pKa = 3.83LARR36 pKa = 11.84QNRR39 pKa = 11.84EE40 pKa = 3.47LRR42 pKa = 11.84AKK44 pKa = 10.83LEE46 pKa = 4.41TKK48 pKa = 10.43GPNRR52 pKa = 11.84PNRR55 pKa = 11.84PKK57 pKa = 10.59LDD59 pKa = 3.4KK60 pKa = 11.19TEE62 pKa = 3.83VAFIKK67 pKa = 10.66DD68 pKa = 3.5LVRR71 pKa = 11.84AGVSRR76 pKa = 11.84RR77 pKa = 11.84DD78 pKa = 3.36VARR81 pKa = 11.84SFDD84 pKa = 4.06VNPSTISRR92 pKa = 11.84IVRR95 pKa = 11.84GQYY98 pKa = 9.84HH99 pKa = 6.14RR100 pKa = 4.58

Molecular weight: 11.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

87

0

87

16056

26

819

184.6

20.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.635 ± 0.333

0.828 ± 0.111

6.222 ± 0.23

6.596 ± 0.286

3.245 ± 0.218

8.994 ± 0.58

2.012 ± 0.179

4.652 ± 0.167

4.397 ± 0.27

8.458 ± 0.249

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.46 ± 0.148

3.301 ± 0.194

5.786 ± 0.368

3.494 ± 0.207

6.863 ± 0.381

5.35 ± 0.169

5.655 ± 0.251

7.113 ± 0.198

2.043 ± 0.147

2.896 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski