Singapore grouper iridovirus

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Pimascovirales; Iridoviridae; Alphairidovirinae; Ranavirus

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 162 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5YFK3|Q5YFK3_9VIRU IlGF domain-containing protein OS=Singapore grouper iridovirus OX=262968 GN=ORF062R PE=4 SV=1
MM1 pKa = 7.44GSGVVSGDD9 pKa = 3.4SCVAAGDD16 pKa = 4.01DD17 pKa = 3.59VGDD20 pKa = 3.98FSVVSSYY27 pKa = 10.72IPEE30 pKa = 4.55GSWVAWGEE38 pKa = 4.05EE39 pKa = 4.45GSWVAWGEE47 pKa = 4.05EE48 pKa = 4.45GSWVAWGEE56 pKa = 4.05EE57 pKa = 4.45GSWVAWGEE65 pKa = 4.05EE66 pKa = 4.45GSWVAWGEE74 pKa = 4.05EE75 pKa = 4.45GSWVAWGEE83 pKa = 4.05EE84 pKa = 4.45GSWVAWGEE92 pKa = 4.05EE93 pKa = 4.45GSWVAWGEE101 pKa = 4.05EE102 pKa = 4.45GSWVAWGEE110 pKa = 4.05EE111 pKa = 4.45GSWVAWGEE119 pKa = 3.91EE120 pKa = 4.37GSWVISSVIPKK131 pKa = 7.74DD132 pKa = 3.76TPISGATVEE141 pKa = 4.35GATVEE146 pKa = 4.25GATVDD151 pKa = 4.28FLGLQYY157 pKa = 10.66RR158 pKa = 11.84SNSFSSHH165 pKa = 5.98SFLQHH170 pKa = 5.56ASARR174 pKa = 11.84PTLRR178 pKa = 11.84VLLPLLGGVYY188 pKa = 9.8TPLSPQCSVSASINSPPHH206 pKa = 5.02TCSS209 pKa = 2.98

Molecular weight: 22.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5YFG1|Q5YFG1_9VIRU DNA-directed RNA polymerase subunit OS=Singapore grouper iridovirus OX=262968 GN=ORF104L PE=3 SV=1
MM1 pKa = 7.75RR2 pKa = 11.84AAQASSAGRR11 pKa = 11.84QSTYY15 pKa = 9.5PAEE18 pKa = 4.51NGLHH22 pKa = 5.69VSHH25 pKa = 7.42LLTINCTLSVNSAAASSPYY44 pKa = 10.57SKK46 pKa = 10.56YY47 pKa = 10.44SNFMIVFKK55 pKa = 10.54IFLVTCPLTILPAVMTASLHH75 pKa = 6.0FKK77 pKa = 10.44LKK79 pKa = 10.7SRR81 pKa = 11.84GSNSSNKK88 pKa = 9.51PKK90 pKa = 10.92GDD92 pKa = 3.42VASAAGPVEE101 pKa = 4.25ASHH104 pKa = 6.91PSIPNASNKK113 pKa = 7.5QHH115 pKa = 6.42ALSSRR120 pKa = 11.84GLGSRR125 pKa = 11.84FILMRR130 pKa = 11.84CAPSTASCTNTTSADD145 pKa = 3.58GPRR148 pKa = 11.84VLIMTLRR155 pKa = 11.84GAINVNVSAMAWGPCGKK172 pKa = 9.84YY173 pKa = 9.81RR174 pKa = 11.84LPSSPSKK181 pKa = 11.38SMLKK185 pKa = 10.22PAVTDD190 pKa = 3.52RR191 pKa = 11.84LSRR194 pKa = 11.84MVFPGSRR201 pKa = 11.84FTLIPSIEE209 pKa = 3.94LRR211 pKa = 11.84CRR213 pKa = 11.84VGCLLRR219 pKa = 11.84ATEE222 pKa = 4.45SPSSKK227 pKa = 9.16TRR229 pKa = 11.84STVIPGSNLGHH240 pKa = 6.36SNSSPRR246 pKa = 11.84SFTRR250 pKa = 11.84PVTWHH255 pKa = 5.82RR256 pKa = 11.84TTTFFRR262 pKa = 11.84AAVLAGCVTLPSSLPHH278 pKa = 5.69SVSMM282 pKa = 5.16

Molecular weight: 29.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

162

0

162

46194

41

1268

285.1

31.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.293 ± 0.408

2.36 ± 0.15

5.059 ± 0.147

6.029 ± 0.283

3.695 ± 0.18

6.932 ± 0.419

2.054 ± 0.101

4.674 ± 0.164

6.053 ± 0.199

8.168 ± 0.3

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.782 ± 0.109

3.931 ± 0.166

6.107 ± 0.38

2.99 ± 0.145

5.494 ± 0.243

7.306 ± 0.356

6.291 ± 0.186

7.113 ± 0.213

1.442 ± 0.129

3.228 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski