Clostridium sp. CAG:307

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1655 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7GE95|R7GE95_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:307 OX=1262795 GN=BN598_00178 PE=4 SV=1
MM1 pKa = 8.05EE2 pKa = 6.27DD3 pKa = 3.08RR4 pKa = 11.84TLTITTDD11 pKa = 2.98SGEE14 pKa = 4.34EE15 pKa = 3.83ILCEE19 pKa = 3.93ILFTTHH25 pKa = 6.85SEE27 pKa = 4.23EE28 pKa = 4.02FNKK31 pKa = 10.71DD32 pKa = 3.14YY33 pKa = 11.42VVFVEE38 pKa = 4.8KK39 pKa = 9.93GTNTASAAIYY49 pKa = 9.15NANDD53 pKa = 3.34KK54 pKa = 11.68GEE56 pKa = 4.23GKK58 pKa = 8.48LTQITSEE65 pKa = 4.46EE66 pKa = 4.36EE67 pKa = 3.6WTMLEE72 pKa = 4.44EE73 pKa = 4.73MLDD76 pKa = 3.6DD77 pKa = 4.07YY78 pKa = 11.95YY79 pKa = 11.94NNQEE83 pKa = 4.13NGCGGSCEE91 pKa = 4.34SCSGCDD97 pKa = 3.42EE98 pKa = 4.71TIEE101 pKa = 4.44DD102 pKa = 3.83

Molecular weight: 11.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7GJ17|R7GJ17_9CLOT 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase OS=Clostridium sp. CAG:307 OX=1262795 GN=ispE PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.31QPNKK9 pKa = 8.55HH10 pKa = 5.61KK11 pKa = 10.83RR12 pKa = 11.84KK13 pKa = 7.42VTHH16 pKa = 6.15GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSVGGRR28 pKa = 11.84LVIKK32 pKa = 10.47RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.28GRR39 pKa = 11.84KK40 pKa = 8.68RR41 pKa = 11.84LSKK44 pKa = 10.84

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1655

0

1655

538369

32

8184

325.3

36.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.675 ± 0.06

1.327 ± 0.023

6.008 ± 0.042

6.549 ± 0.063

4.592 ± 0.048

5.822 ± 0.056

1.627 ± 0.027

9.44 ± 0.077

8.578 ± 0.094

9.506 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.465 ± 0.029

6.756 ± 0.065

2.662 ± 0.035

2.284 ± 0.03

3.099 ± 0.042

6.615 ± 0.068

5.284 ± 0.089

5.975 ± 0.047

0.564 ± 0.018

5.17 ± 0.053
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski