Avon-Heathcote Estuary associated circular virus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IBG0|A0A0C5IBG0_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 2 OX=1618243 PE=4 SV=1
MM1 pKa = 7.8PRR3 pKa = 11.84GQLTKK8 pKa = 10.03TEE10 pKa = 4.06KK11 pKa = 10.51KK12 pKa = 8.66QVKK15 pKa = 10.36NIVQSQLSKK24 pKa = 9.7VTEE27 pKa = 4.44LKK29 pKa = 9.66QHH31 pKa = 4.99VVEE34 pKa = 5.11FDD36 pKa = 3.4YY37 pKa = 11.59DD38 pKa = 3.65PVFRR42 pKa = 11.84LNAGGVFHH50 pKa = 7.08CLSNVQLNTSSPAGMTDD67 pKa = 2.78ATRR70 pKa = 11.84NEE72 pKa = 4.08RR73 pKa = 11.84FGSKK77 pKa = 9.86ISPKK81 pKa = 10.48SITVDD86 pKa = 3.14LMLRR90 pKa = 11.84GSRR93 pKa = 11.84DD94 pKa = 3.5SVGFSGKK101 pKa = 8.11TVDD104 pKa = 4.12EE105 pKa = 4.06IRR107 pKa = 11.84VVLFRR112 pKa = 11.84WKK114 pKa = 11.03NEE116 pKa = 3.63TDD118 pKa = 3.57VVAGGTKK125 pKa = 10.17FPTQGLLWGEE135 pKa = 4.38TSPDD139 pKa = 3.07QYY141 pKa = 11.89NILTKK146 pKa = 10.0PLNVFGDD153 pKa = 3.48KK154 pKa = 10.82DD155 pKa = 3.81FEE157 pKa = 4.28IVEE160 pKa = 4.12DD161 pKa = 4.23RR162 pKa = 11.84KK163 pKa = 10.77IKK165 pKa = 9.76LTGDD169 pKa = 3.31ASQYY173 pKa = 9.38MSGVSLISHH182 pKa = 6.57TNSSQNKK189 pKa = 9.58DD190 pKa = 2.75KK191 pKa = 11.08LLRR194 pKa = 11.84LKK196 pKa = 10.74VPYY199 pKa = 10.08KK200 pKa = 10.83DD201 pKa = 3.13IVKK204 pKa = 8.95TLNFVDD210 pKa = 4.11SQVGTGFHH218 pKa = 5.67TAEE221 pKa = 4.38HH222 pKa = 6.61RR223 pKa = 11.84NSYY226 pKa = 8.23WLYY229 pKa = 9.98IATDD233 pKa = 3.96NNIMGHH239 pKa = 6.01PVTMNLPYY247 pKa = 8.62YY248 pKa = 9.33TLYY251 pKa = 11.3SRR253 pKa = 11.84MNFTDD258 pKa = 3.17IGAA261 pKa = 3.97

Molecular weight: 29.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IBG0|A0A0C5IBG0_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 2 OX=1618243 PE=4 SV=1
MM1 pKa = 7.06KK2 pKa = 10.62QNTKK6 pKa = 10.55KK7 pKa = 9.86EE8 pKa = 3.75LRR10 pKa = 11.84YY11 pKa = 9.88RR12 pKa = 11.84RR13 pKa = 11.84WCITEE18 pKa = 3.93NNYY21 pKa = 9.27TDD23 pKa = 3.72EE24 pKa = 4.6SIKK27 pKa = 10.5ILSEE31 pKa = 3.92LPTNYY36 pKa = 9.7IIVGKK41 pKa = 10.37EE42 pKa = 3.08IGKK45 pKa = 9.84KK46 pKa = 10.09KK47 pKa = 9.02KK48 pKa = 8.64TPHH51 pKa = 5.18LQIYY55 pKa = 10.43LEE57 pKa = 4.1FVNAKK62 pKa = 8.55TFSAVKK68 pKa = 10.26KK69 pKa = 9.49LLKK72 pKa = 9.7KK73 pKa = 10.64AHH75 pKa = 6.46IEE77 pKa = 4.04VSKK80 pKa = 11.42GNAKK84 pKa = 10.34QNTDD88 pKa = 3.67YY89 pKa = 11.02CSKK92 pKa = 10.48DD93 pKa = 3.02QDD95 pKa = 4.04VILVRR100 pKa = 11.84GEE102 pKa = 3.97PFNQGKK108 pKa = 10.29RR109 pKa = 11.84NDD111 pKa = 3.66LSCIKK116 pKa = 10.38EE117 pKa = 4.83LIQQDD122 pKa = 3.77YY123 pKa = 10.55NIRR126 pKa = 11.84EE127 pKa = 4.12MLEE130 pKa = 3.92EE131 pKa = 4.01EE132 pKa = 4.86SIKK135 pKa = 10.7SLQGLRR141 pKa = 11.84MAEE144 pKa = 3.7NLMKK148 pKa = 10.66YY149 pKa = 8.46VEE151 pKa = 4.58KK152 pKa = 10.4PRR154 pKa = 11.84SKK156 pKa = 10.68APNIIWRR163 pKa = 11.84YY164 pKa = 7.33GASGSGKK171 pKa = 7.41TRR173 pKa = 11.84WVYY176 pKa = 10.97EE177 pKa = 3.69NYY179 pKa = 10.11TEE181 pKa = 4.35VFCPINFKK189 pKa = 9.01WWEE192 pKa = 4.39GYY194 pKa = 9.96DD195 pKa = 3.43GHH197 pKa = 7.8KK198 pKa = 10.7VVLLDD203 pKa = 4.13DD204 pKa = 4.42LRR206 pKa = 11.84GDD208 pKa = 3.46FCKK211 pKa = 10.65YY212 pKa = 10.56HH213 pKa = 6.95EE214 pKa = 4.43FLKK217 pKa = 10.3LTDD220 pKa = 4.02RR221 pKa = 11.84YY222 pKa = 9.73PYY224 pKa = 10.22RR225 pKa = 11.84VEE227 pKa = 4.09CKK229 pKa = 10.25GGSRR233 pKa = 11.84QLLADD238 pKa = 4.4TIIITSPVHH247 pKa = 6.33PKK249 pKa = 10.1EE250 pKa = 4.0VWDD253 pKa = 4.26TIEE256 pKa = 5.68DD257 pKa = 3.64KK258 pKa = 11.06KK259 pKa = 11.15QLLRR263 pKa = 11.84RR264 pKa = 11.84ITKK267 pKa = 9.07IDD269 pKa = 3.64HH270 pKa = 5.67VVEE273 pKa = 4.5NYY275 pKa = 10.18TEE277 pKa = 4.23VKK279 pKa = 10.35RR280 pKa = 11.84GNTT283 pKa = 3.38

Molecular weight: 33.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

544

261

283

272.0

31.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.941 ± 0.088

1.287 ± 0.642

5.882 ± 0.448

6.25 ± 1.718

3.493 ± 0.785

6.25 ± 0.731

2.206 ± 0.066

6.25 ± 1.174

9.926 ± 1.608

8.272 ± 0.112

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.022 ± 0.469

5.882 ± 0.176

3.493 ± 0.241

3.86 ± 0.252

5.331 ± 0.521

6.25 ± 1.276

6.985 ± 1.025

7.353 ± 1.036

1.654 ± 0.359

4.412 ± 0.684
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski