Listeria phage B054

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8ATM6|A8ATM6_9CAUD Gp68 OS=Listeria phage B054 OX=330397 PE=4 SV=1
MM1 pKa = 7.71SEE3 pKa = 3.97TTEE6 pKa = 4.33MILEE10 pKa = 5.05GILCRR15 pKa = 11.84NCGVFIDD22 pKa = 4.87DD23 pKa = 4.44VNPGYY28 pKa = 9.9PRR30 pKa = 11.84TCEE33 pKa = 3.84DD34 pKa = 4.91CEE36 pKa = 4.94DD37 pKa = 4.15CEE39 pKa = 4.43EE40 pKa = 4.84DD41 pKa = 4.03NNEE44 pKa = 3.94KK45 pKa = 10.6RR46 pKa = 3.83

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8ATN2|A8ATN2_9CAUD Gp74 OS=Listeria phage B054 OX=330397 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 9.77FLKK5 pKa = 10.05MSEE8 pKa = 3.89FRR10 pKa = 11.84LLSTRR15 pKa = 11.84IFNKK19 pKa = 9.26PAFYY23 pKa = 10.29RR24 pKa = 11.84IKK26 pKa = 10.99LNFLLTFYY34 pKa = 11.24RR35 pKa = 11.84LFTGQKK41 pKa = 7.29TRR43 pKa = 11.84NSVVRR48 pKa = 11.84CYY50 pKa = 10.66CQEE53 pKa = 3.81NKK55 pKa = 10.22KK56 pKa = 10.66

Molecular weight: 6.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

15352

38

1571

191.9

21.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.256 ± 0.457

0.69 ± 0.102

5.719 ± 0.269

8.253 ± 0.317

4.019 ± 0.168

5.791 ± 0.513

1.518 ± 0.14

7.523 ± 0.278

9.419 ± 0.383

8.084 ± 0.381

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.365 ± 0.122

6.162 ± 0.225

2.716 ± 0.156

3.465 ± 0.182

4.325 ± 0.271

6.501 ± 0.289

5.713 ± 0.21

5.934 ± 0.233

1.088 ± 0.097

3.459 ± 0.278
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski