Macrophomina phaseolina (strain MS6) (Charcoal rot fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetes incertae sedis; Botryosphaeriales; Botryosphaeriaceae; Macrophomina; Macrophomina phaseolina

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13803 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K2S100|K2S100_MACPH Ribonuclease H-like protein OS=Macrophomina phaseolina (strain MS6) OX=1126212 GN=MPH_04142 PE=4 SV=1
MM1 pKa = 7.67LKK3 pKa = 10.05QLTLVALATTAFAQTQDD20 pKa = 3.64LNATLTSTPEE30 pKa = 3.88LSNLTTYY37 pKa = 11.26LGMFPDD43 pKa = 3.88ILTTLSGVSNITILAPNNDD62 pKa = 3.54AFGKK66 pKa = 10.47LLASPIGSQLASNPALIQSVLTYY89 pKa = 10.35HH90 pKa = 6.25VLNGTYY96 pKa = 9.64TSNQITDD103 pKa = 3.81DD104 pKa = 3.67VAFIPTLLTDD114 pKa = 3.9PAYY117 pKa = 10.76ANVTGGQRR125 pKa = 11.84VGVEE129 pKa = 4.14TEE131 pKa = 3.98DD132 pKa = 4.19DD133 pKa = 3.51NVIIYY138 pKa = 10.58SGLLQNSTVTQADD151 pKa = 3.63VQFTGGVVHH160 pKa = 7.57IIDD163 pKa = 3.88TVLTLPANASTTAIAANLTALAGALTTANLVSAVDD198 pKa = 3.56TARR201 pKa = 11.84DD202 pKa = 3.39ITVFAPTNAAFEE214 pKa = 5.08AIGSVLANATTQQLTDD230 pKa = 3.2VLTYY234 pKa = 10.36HH235 pKa = 6.03VVNGTVAYY243 pKa = 8.01STTLQPGEE251 pKa = 4.08QTVTTLNGGQLTIRR265 pKa = 11.84VQDD268 pKa = 4.27DD269 pKa = 3.31GDD271 pKa = 3.96VFVNGARR278 pKa = 11.84VVLTDD283 pKa = 3.12VLIANGVVHH292 pKa = 7.15VIDD295 pKa = 4.69NVLNPANATAAPPTGDD311 pKa = 3.63NADD314 pKa = 3.81DD315 pKa = 3.74TGSVQFGGASSGNVPFTAGIPSPTGTIGGGNLSSGNATPTAGTQPSSGAMANTVISGGLMGIAIGLGGVVLGLL388 pKa = 3.79

Molecular weight: 39.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K2SE33|K2SE33_MACPH WW/Rsp5/WWP OS=Macrophomina phaseolina (strain MS6) OX=1126212 GN=MPH_02029 PE=4 SV=1
MM1 pKa = 7.78LSTLAPRR8 pKa = 11.84PTLSSATPTSRR19 pKa = 11.84TRR21 pKa = 11.84MTALLSTPARR31 pKa = 11.84TLPSPAAPALVATVSPSAPLVAAPTTLSRR60 pKa = 11.84ASRR63 pKa = 11.84SRR65 pKa = 11.84TLPSRR70 pKa = 11.84TPTTVSASRR79 pKa = 11.84PSTAPPALSPVSPTAASPSRR99 pKa = 11.84TLPSMVSSLSRR110 pKa = 11.84TTRR113 pKa = 11.84TAPPLASLPAAFRR126 pKa = 11.84SPTSPSPTSRR136 pKa = 11.84APSPPAVPMSTSSALLVPAPTGSGPVSRR164 pKa = 11.84SLVARR169 pKa = 11.84RR170 pKa = 11.84APSAPAFPAALALLAKK186 pKa = 10.12CLVPVARR193 pKa = 11.84RR194 pKa = 11.84VAALPKK200 pKa = 9.76RR201 pKa = 11.84VHH203 pKa = 6.32FSSKK207 pKa = 10.23VKK209 pKa = 10.69GLTTEE214 pKa = 4.41GFLGSS219 pKa = 3.71

Molecular weight: 22.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13803

0

13803

5914650

8

10745

428.5

47.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.403 ± 0.022

1.271 ± 0.009

5.578 ± 0.016

6.181 ± 0.023

3.727 ± 0.013

7.016 ± 0.022

2.414 ± 0.01

4.57 ± 0.014

4.68 ± 0.02

8.807 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.099 ± 0.008

3.523 ± 0.013

6.141 ± 0.025

3.937 ± 0.018

6.357 ± 0.021

8.052 ± 0.026

5.842 ± 0.026

6.148 ± 0.018

1.513 ± 0.008

2.741 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski