Anaerotruncus sp. G3(2012)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Anaerotruncus; unclassified Anaerotruncus

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3406 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9L9F1|R9L9F1_9FIRM Aminotransferase OS=Anaerotruncus sp. G3(2012) OX=1235835 GN=C814_03091 PE=3 SV=1
MM1 pKa = 8.21DD2 pKa = 4.26YY3 pKa = 11.41EE4 pKa = 5.42KK5 pKa = 10.13MDD7 pKa = 3.7SLQIDD12 pKa = 3.91VLRR15 pKa = 11.84EE16 pKa = 3.55IGNIGSGNAATSLSEE31 pKa = 4.41FLSCPVNIGVPSVQILDD48 pKa = 3.54YY49 pKa = 10.61DD50 pKa = 3.96QVVEE54 pKa = 4.12ALGGPEE60 pKa = 3.6TMMVGLLLQLEE71 pKa = 4.37DD72 pKa = 5.35DD73 pKa = 3.93IKK75 pKa = 11.36GMIMFLLHH83 pKa = 7.12KK84 pKa = 9.6EE85 pKa = 4.14FAHH88 pKa = 5.99MALSALMGDD97 pKa = 4.51MYY99 pKa = 11.58NSFDD103 pKa = 3.76EE104 pKa = 4.13MDD106 pKa = 4.89EE107 pKa = 4.09ISASAMQEE115 pKa = 3.74VGNIMAASYY124 pKa = 11.17VNAIADD130 pKa = 3.74MTGLSINISVPSICMDD146 pKa = 3.25MAGAIVSVPAVYY158 pKa = 10.53YY159 pKa = 11.12ADD161 pKa = 3.93ISDD164 pKa = 4.3KK165 pKa = 10.84IIFIGDD171 pKa = 3.36DD172 pKa = 3.62FVRR175 pKa = 11.84DD176 pKa = 4.24DD177 pKa = 3.75SHH179 pKa = 7.31HH180 pKa = 6.13AANFNSHH187 pKa = 5.99ILMIPEE193 pKa = 4.18SDD195 pKa = 3.61SLEE198 pKa = 4.84KK199 pKa = 10.77IMTSLGIEE207 pKa = 3.91II208 pKa = 4.75

Molecular weight: 22.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9LAJ6|R9LAJ6_9FIRM Uncharacterized protein (Fragment) OS=Anaerotruncus sp. G3(2012) OX=1235835 GN=C814_03085 PE=4 SV=1
MM1 pKa = 7.32RR2 pKa = 11.84RR3 pKa = 11.84VYY5 pKa = 10.06PVSLCFFPALVHH17 pKa = 6.87FFTKK21 pKa = 10.65DD22 pKa = 3.27LVVPSASNRR31 pKa = 11.84LGSEE35 pKa = 4.23SSTHH39 pKa = 6.6AYY41 pKa = 9.39LKK43 pKa = 10.44KK44 pKa = 10.21GCLAAAHH51 pKa = 6.69IFCGNKK57 pKa = 9.85APEE60 pKa = 3.91VWIPGCGAFWVPDD73 pKa = 3.32QNAPTRR79 pKa = 11.84GFLAQARR86 pKa = 11.84SAAKK90 pKa = 9.8AATAARR96 pKa = 11.84RR97 pKa = 11.84TPQEE101 pKa = 3.71GLRR104 pKa = 11.84ATEE107 pKa = 3.85VWIPDD112 pKa = 3.62QTSGAQAPFFGGLLGPAARR131 pKa = 11.84RR132 pKa = 11.84ALRR135 pKa = 11.84ATLGQDD141 pKa = 3.07APHH144 pKa = 6.26GRR146 pKa = 11.84GVKK149 pKa = 10.37GSIRR153 pKa = 11.84LAPDD157 pKa = 2.8QTDD160 pKa = 3.01RR161 pKa = 11.84RR162 pKa = 11.84RR163 pKa = 11.84APSSGLLGG171 pKa = 3.77

Molecular weight: 18.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3406

0

3406

991676

22

2436

291.2

32.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.052 ± 0.062

1.611 ± 0.021

5.416 ± 0.035

6.916 ± 0.041

3.971 ± 0.03

7.682 ± 0.045

1.757 ± 0.021

6.03 ± 0.038

4.959 ± 0.043

9.735 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.774 ± 0.023

3.489 ± 0.027

4.269 ± 0.029

3.853 ± 0.029

5.794 ± 0.041

6.035 ± 0.038

5.251 ± 0.045

6.947 ± 0.034

0.975 ± 0.014

3.483 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski