Bacteriophage Lily

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Lilyvirus; Paenibacillus virus Lily

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5AJ96|A0A0C5AJ96_9CAUD ArpU-like transcriptional regulator OS=Bacteriophage Lily OX=1589751 GN=LILY_69 PE=4 SV=1
MM1 pKa = 7.17KK2 pKa = 10.41VKK4 pKa = 9.05YY5 pKa = 8.25TLSIGYY11 pKa = 8.78PGACQEE17 pKa = 4.77DD18 pKa = 4.77EE19 pKa = 4.43IEE21 pKa = 4.87IDD23 pKa = 3.95DD24 pKa = 4.6EE25 pKa = 4.22EE26 pKa = 6.18LEE28 pKa = 4.26GLTPEE33 pKa = 4.15EE34 pKa = 4.16AEE36 pKa = 3.88EE37 pKa = 4.27RR38 pKa = 11.84IYY40 pKa = 10.95DD41 pKa = 3.79IVDD44 pKa = 4.06EE45 pKa = 4.34YY46 pKa = 11.78ALGYY50 pKa = 10.06ISLYY54 pKa = 8.01WEE56 pKa = 4.44RR57 pKa = 11.84VDD59 pKa = 4.38EE60 pKa = 4.37

Molecular weight: 7.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5AEE3|A0A0C5AEE3_9CAUD Uncharacterized protein OS=Bacteriophage Lily OX=1589751 GN=LILY_8 PE=4 SV=1
MM1 pKa = 7.1WTLQEE6 pKa = 4.33AQTHH10 pKa = 5.51LKK12 pKa = 10.11AWLEE16 pKa = 3.96AEE18 pKa = 4.3MAVASAQSYY27 pKa = 8.2TIAGRR32 pKa = 11.84SLTRR36 pKa = 11.84ANLPEE41 pKa = 3.92IRR43 pKa = 11.84KK44 pKa = 9.35QIQFWRR50 pKa = 11.84NEE52 pKa = 3.73IEE54 pKa = 4.55KK55 pKa = 10.76LNNPQRR61 pKa = 11.84RR62 pKa = 11.84SRR64 pKa = 11.84IFRR67 pKa = 11.84GIPMDD72 pKa = 3.18II73 pKa = 4.08

Molecular weight: 8.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

13639

40

837

184.3

20.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.965 ± 0.34

0.916 ± 0.104

5.822 ± 0.175

8.153 ± 0.396

3.754 ± 0.169

6.826 ± 0.35

1.679 ± 0.163

6.606 ± 0.306

8.234 ± 0.347

8.226 ± 0.267

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.727 ± 0.158

4.099 ± 0.167

3.937 ± 0.234

4.062 ± 0.239

4.846 ± 0.292

6.188 ± 0.231

5.294 ± 0.228

6.782 ± 0.323

1.415 ± 0.13

3.468 ± 0.231
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski