Enterobacteria phage IME08

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Dhakavirus; Escherichia virus IME08

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 253 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D7RM91|D7RM91_9CAUD NrdC thioredoxin OS=Enterobacteria phage IME08 OX=697227 GN=nrdC PE=3 SV=1
MM1 pKa = 7.33LVADD5 pKa = 4.8VYY7 pKa = 11.72DD8 pKa = 4.23LLAFISGWCFILEE21 pKa = 4.06LQMTEE26 pKa = 3.59FDD28 pKa = 3.51IAYY31 pKa = 6.68GTCASEE37 pKa = 4.46FDD39 pKa = 3.66TPAPSKK45 pKa = 10.0EE46 pKa = 4.21TVLANLPDD54 pKa = 3.73WLKK57 pKa = 11.3EE58 pKa = 3.94EE59 pKa = 4.21ANEE62 pKa = 4.17WGWTDD67 pKa = 3.1TCVRR71 pKa = 11.84DD72 pKa = 3.46QLFEE76 pKa = 4.26FMVEE80 pKa = 3.99LL81 pKa = 4.58

Molecular weight: 9.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D7RML5|D7RML5_9CAUD Gp31 head assembly cochaperone with GroEL OS=Enterobacteria phage IME08 OX=697227 GN=31 PE=4 SV=1
MM1 pKa = 7.53AKK3 pKa = 10.16KK4 pKa = 9.46EE5 pKa = 4.17VKK7 pKa = 10.55VVDD10 pKa = 4.16GTSKK14 pKa = 9.78RR15 pKa = 11.84AGYY18 pKa = 9.8KK19 pKa = 9.18RR20 pKa = 11.84ASNKK24 pKa = 10.11RR25 pKa = 11.84INQVVDD31 pKa = 3.24KK32 pKa = 10.27LQARR36 pKa = 11.84ARR38 pKa = 11.84AVLRR42 pKa = 11.84ADD44 pKa = 3.85AACFGKK50 pKa = 9.41PQAA53 pKa = 4.58

Molecular weight: 5.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

251

2

253

53978

46

1299

213.4

24.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.899 ± 0.191

1.039 ± 0.075

6.269 ± 0.108

7.179 ± 0.16

4.205 ± 0.119

6.395 ± 0.224

1.641 ± 0.081

6.856 ± 0.133

7.388 ± 0.195

7.736 ± 0.161

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.044 ± 0.103

5.447 ± 0.127

3.576 ± 0.111

3.513 ± 0.111

4.393 ± 0.104

6.282 ± 0.126

5.941 ± 0.192

6.642 ± 0.117

1.36 ± 0.062

4.194 ± 0.116
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski