Varroa destructor virus 3

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0CQB9|A0A1J0CQB9_9VIRU RNA-directed RNA polymerase OS=Varroa destructor virus 3 OX=2056385 PE=4 SV=1
MM1 pKa = 7.31NCIEE5 pKa = 4.15LQYY8 pKa = 10.93TFDD11 pKa = 3.68LRR13 pKa = 11.84EE14 pKa = 3.59MRR16 pKa = 11.84FDD18 pKa = 5.4HH19 pKa = 7.31IICTLIWTMALIFLVVNGVLGLEE42 pKa = 3.89YY43 pKa = 10.22RR44 pKa = 11.84IKK46 pKa = 10.89NIMYY50 pKa = 10.94NMFLLDD56 pKa = 3.75APKK59 pKa = 8.5TTIYY63 pKa = 10.66EE64 pKa = 4.03DD65 pKa = 3.36TAMLEE70 pKa = 4.24SMKK73 pKa = 10.61VNPLVTAHH81 pKa = 7.64PDD83 pKa = 3.58TFPSCQLMVLTCNDD97 pKa = 2.9QGVWCRR103 pKa = 11.84AGNGFRR109 pKa = 11.84ISKK112 pKa = 8.41TIVVPTHH119 pKa = 6.51VIDD122 pKa = 4.29TSAFTAKK129 pKa = 9.89YY130 pKa = 9.79VRR132 pKa = 11.84LAVPEE137 pKa = 4.25GKK139 pKa = 10.1RR140 pKa = 11.84LGKK143 pKa = 9.23MVVVEE148 pKa = 4.32TPIKK152 pKa = 10.53DD153 pKa = 3.07ILTIGDD159 pKa = 4.94DD160 pKa = 3.12ISLINISEE168 pKa = 4.14IEE170 pKa = 4.12LNRR173 pKa = 11.84LGVTAARR180 pKa = 11.84IKK182 pKa = 10.04TLDD185 pKa = 3.45TKK187 pKa = 9.6QTSVMAMIYY196 pKa = 10.13SYY198 pKa = 11.78APLFKK203 pKa = 10.78ASVGKK208 pKa = 10.62ISPFDD213 pKa = 3.5GLAQQIVFSGSTIQGFSGALYY234 pKa = 10.23VAGKK238 pKa = 9.55NDD240 pKa = 3.66VYY242 pKa = 11.53GMHH245 pKa = 6.83TSGGAVNFGISASYY259 pKa = 10.24LQLLVLGTEE268 pKa = 4.39SNDD271 pKa = 3.94TIIKK275 pKa = 10.06SQDD278 pKa = 3.22NPLSRR283 pKa = 11.84LIKK286 pKa = 10.35RR287 pKa = 11.84FMEE290 pKa = 4.3LEE292 pKa = 4.31NGSSVTPEE300 pKa = 3.56SRR302 pKa = 11.84GGYY305 pKa = 9.64SSDD308 pKa = 3.19RR309 pKa = 11.84TLEE312 pKa = 4.03EE313 pKa = 5.39FLSDD317 pKa = 3.17WDD319 pKa = 3.79NKK321 pKa = 10.66KK322 pKa = 10.23KK323 pKa = 11.05GKK325 pKa = 9.75IGRR328 pKa = 11.84NWNDD332 pKa = 2.92SLGAYY337 pKa = 9.32VYY339 pKa = 10.41SIEE342 pKa = 3.88GRR344 pKa = 11.84FYY346 pKa = 11.21NITEE350 pKa = 5.13DD351 pKa = 3.24IDD353 pKa = 3.73NYY355 pKa = 10.04AQIRR359 pKa = 11.84FPHH362 pKa = 6.44LFEE365 pKa = 6.01DD366 pKa = 4.98ADD368 pKa = 3.98FEE370 pKa = 5.18YY371 pKa = 9.18DD372 pKa = 3.3THH374 pKa = 8.38DD375 pKa = 3.43EE376 pKa = 4.34NGDD379 pKa = 3.05KK380 pKa = 10.41RR381 pKa = 11.84VRR383 pKa = 11.84HH384 pKa = 5.62NKK386 pKa = 8.65FSKK389 pKa = 9.67SKK391 pKa = 10.29RR392 pKa = 11.84YY393 pKa = 10.0ANPIEE398 pKa = 4.97DD399 pKa = 4.98EE400 pKa = 4.37IPVEE404 pKa = 4.5DD405 pKa = 3.69IYY407 pKa = 11.43EE408 pKa = 4.13EE409 pKa = 4.18EE410 pKa = 3.84EE411 pKa = 4.23EE412 pKa = 4.23YY413 pKa = 10.81RR414 pKa = 11.84LEE416 pKa = 3.61EE417 pKa = 3.88RR418 pKa = 11.84RR419 pKa = 11.84RR420 pKa = 11.84KK421 pKa = 9.7KK422 pKa = 10.84VEE424 pKa = 3.26FDD426 pKa = 3.12EE427 pKa = 5.7FVRR430 pKa = 11.84NMNWPVVLGGLMNQLLSEE448 pKa = 4.27

Molecular weight: 50.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0CQC2|A0A1J0CQC2_9VIRU ORF2 OS=Varroa destructor virus 3 OX=2056385 PE=4 SV=1
MM1 pKa = 7.37KK2 pKa = 10.43RR3 pKa = 11.84LLIQEE8 pKa = 4.69ANANPNGGYY17 pKa = 9.94KK18 pKa = 10.18DD19 pKa = 4.05DD20 pKa = 4.28VLMEE24 pKa = 5.11PIFHH28 pKa = 7.09GDD30 pKa = 3.18SEE32 pKa = 5.05AEE34 pKa = 4.05LLADD38 pKa = 4.19YY39 pKa = 10.67NQMVAPMLVEE49 pKa = 5.4DD50 pKa = 4.59EE51 pKa = 4.96LDD53 pKa = 3.58LLRR56 pKa = 11.84EE57 pKa = 4.28TVNKK61 pKa = 10.62SEE63 pKa = 3.96VSYY66 pKa = 11.56EE67 pKa = 3.89EE68 pKa = 4.0VQSIRR73 pKa = 11.84SICNRR78 pKa = 11.84LTQLGKK84 pKa = 10.56FSNQLLSTIDD94 pKa = 4.11KK95 pKa = 10.5IRR97 pKa = 11.84ADD99 pKa = 3.33KK100 pKa = 10.58KK101 pKa = 10.14KK102 pKa = 10.74RR103 pKa = 11.84EE104 pKa = 4.11AEE106 pKa = 3.69EE107 pKa = 3.64RR108 pKa = 11.84ARR110 pKa = 11.84QIEE113 pKa = 4.42MEE115 pKa = 4.02LKK117 pKa = 8.84MRR119 pKa = 11.84KK120 pKa = 9.21KK121 pKa = 10.71EE122 pKa = 3.97EE123 pKa = 3.89LKK125 pKa = 10.67KK126 pKa = 10.48RR127 pKa = 11.84AIEE130 pKa = 4.04MEE132 pKa = 4.13EE133 pKa = 3.67SRR135 pKa = 11.84ARR137 pKa = 11.84QEE139 pKa = 4.19SEE141 pKa = 4.0AKK143 pKa = 9.13EE144 pKa = 4.22KK145 pKa = 10.16EE146 pKa = 4.47VKK148 pKa = 10.25RR149 pKa = 11.84SAMCLRR155 pKa = 11.84VRR157 pKa = 11.84EE158 pKa = 4.2LFEE161 pKa = 4.39SLTEE165 pKa = 3.96EE166 pKa = 4.3EE167 pKa = 4.05IKK169 pKa = 10.73EE170 pKa = 3.89FSRR173 pKa = 11.84LVVEE177 pKa = 4.99RR178 pKa = 11.84NTKK181 pKa = 9.84RR182 pKa = 11.84ALARR186 pKa = 11.84KK187 pKa = 8.95SEE189 pKa = 4.11KK190 pKa = 10.16EE191 pKa = 3.75KK192 pKa = 10.25MRR194 pKa = 11.84QEE196 pKa = 4.27VISAIDD202 pKa = 3.47RR203 pKa = 11.84PEE205 pKa = 3.88EE206 pKa = 4.13KK207 pKa = 9.88SDD209 pKa = 3.41KK210 pKa = 10.85KK211 pKa = 10.77IVLEE215 pKa = 4.14GTKK218 pKa = 10.73LKK220 pKa = 9.48DD221 pKa = 3.24TKK223 pKa = 11.15SSVKK227 pKa = 10.26EE228 pKa = 4.36DD229 pKa = 3.01IRR231 pKa = 11.84PPPKK235 pKa = 10.74GMIRR239 pKa = 11.84CPSCIIRR246 pKa = 11.84KK247 pKa = 9.72AKK249 pKa = 10.23DD250 pKa = 3.4RR251 pKa = 11.84PCFSNKK257 pKa = 9.24PDD259 pKa = 3.67FFKK262 pKa = 11.01HH263 pKa = 4.91MEE265 pKa = 4.25EE266 pKa = 4.01VHH268 pKa = 5.33LTRR271 pKa = 11.84LEE273 pKa = 4.12AAPSGNN279 pKa = 3.3

Molecular weight: 32.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1220

279

493

406.7

46.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.393 ± 0.802

1.639 ± 0.265

5.902 ± 0.31

8.934 ± 1.713

4.344 ± 0.557

5.656 ± 0.935

2.049 ± 0.516

5.738 ± 1.134

7.049 ± 1.419

9.344 ± 0.521

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.443 ± 0.152

4.426 ± 0.551

4.098 ± 0.439

2.459 ± 0.138

6.639 ± 0.836

6.311 ± 0.435

4.262 ± 0.842

6.803 ± 0.579

1.557 ± 0.681

2.951 ± 0.682
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski