Mycobacterium phage D29 (Mycobacteriophage D29)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|O64252|PRXH_BPMD2 Putative non-heme haloperoxidase OS=Mycobacterium phage D29 OX=28369 GN=59.2 PE=3 SV=1
MM1 pKa = 7.25NPVLFALDD9 pKa = 3.38AHH11 pKa = 6.96IIACAVLSYY20 pKa = 10.87FCLLMDD26 pKa = 4.48NPKK29 pKa = 10.8GSDD32 pKa = 3.51GEE34 pKa = 4.36SRR36 pKa = 11.84EE37 pKa = 4.03EE38 pKa = 3.94DD39 pKa = 3.33SGVPGAEE46 pKa = 4.02SS47 pKa = 2.94

Molecular weight: 4.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|O64201|VG05_BPMD2 Gene 5 protein OS=Mycobacterium phage D29 OX=28369 GN=5 PE=4 SV=1
MM1 pKa = 7.26SWAGSKK7 pKa = 9.81RR8 pKa = 11.84RR9 pKa = 11.84QEE11 pKa = 4.41LPEE14 pKa = 4.36DD15 pKa = 3.29WEE17 pKa = 4.35LNYY20 pKa = 10.51RR21 pKa = 11.84LPVLSAAGWLCEE33 pKa = 3.95VDD35 pKa = 4.35GPGCVRR41 pKa = 11.84AATDD45 pKa = 3.4VDD47 pKa = 3.89HH48 pKa = 7.26KK49 pKa = 11.42KK50 pKa = 10.38PGNDD54 pKa = 3.02HH55 pKa = 6.58SRR57 pKa = 11.84SNLQAICRR65 pKa = 11.84VCHH68 pKa = 5.79GKK70 pKa = 10.33KK71 pKa = 9.87SAAEE75 pKa = 3.95GVARR79 pKa = 11.84RR80 pKa = 11.84RR81 pKa = 11.84EE82 pKa = 3.88LKK84 pKa = 10.3ARR86 pKa = 11.84RR87 pKa = 11.84KK88 pKa = 9.96RR89 pKa = 11.84PEE91 pKa = 3.3QRR93 pKa = 11.84HH94 pKa = 5.2PGRR97 pKa = 11.84RR98 pKa = 3.42

Molecular weight: 11.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

14885

31

837

193.3

21.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.064 ± 0.371

0.867 ± 0.133

6.47 ± 0.23

6.779 ± 0.281

3.52 ± 0.165

8.21 ± 0.411

2.157 ± 0.197

5.059 ± 0.178

4.582 ± 0.258

8.257 ± 0.308

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.311 ± 0.124

3.238 ± 0.165

5.408 ± 0.228

3.514 ± 0.218

6.456 ± 0.359

5.045 ± 0.229

5.858 ± 0.243

7.269 ± 0.185

2.022 ± 0.144

2.916 ± 0.196
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski