Streptomyces phage Attoomi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Attoomivirus; Streptomyces virus Attoomi

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5BLJ4|A0A2H5BLJ4_9CAUD Uncharacterized protein OS=Streptomyces phage Attoomi OX=2059881 GN=SEA_ATTOOMI_41 PE=4 SV=1
MM1 pKa = 7.81AEE3 pKa = 4.21TFGCDD8 pKa = 2.61WDD10 pKa = 4.38GNQAVTVDD18 pKa = 3.57HH19 pKa = 6.74DD20 pKa = 4.21TEE22 pKa = 4.24HH23 pKa = 8.25VEE25 pKa = 4.36VNLHH29 pKa = 5.48EE30 pKa = 4.52VGEE33 pKa = 4.63PGDD36 pKa = 3.76ATCVYY41 pKa = 9.8LAPEE45 pKa = 4.03VARR48 pKa = 11.84RR49 pKa = 11.84VARR52 pKa = 11.84AITLASFAVEE62 pKa = 4.25GQDD65 pKa = 3.21VTDD68 pKa = 4.34ALFVSPEE75 pKa = 3.87ALEE78 pKa = 4.09AATRR82 pKa = 11.84AEE84 pKa = 4.79GGVEE88 pKa = 4.16MPDD91 pKa = 4.36AIPVEE96 pKa = 4.52PCPEE100 pKa = 4.24TNVGCDD106 pKa = 3.32PVCPSRR112 pKa = 11.84EE113 pKa = 4.11DD114 pKa = 3.53CRR116 pKa = 11.84EE117 pKa = 3.54SAMRR121 pKa = 11.84DD122 pKa = 3.56LFRR125 pKa = 11.84EE126 pKa = 4.04PDD128 pKa = 3.8MEE130 pKa = 4.35PVPYY134 pKa = 10.78ALVDD138 pKa = 3.45EE139 pKa = 4.95HH140 pKa = 7.06QDD142 pKa = 3.22RR143 pKa = 11.84ADD145 pKa = 3.27AFLAARR151 pKa = 11.84EE152 pKa = 4.11LAGPAANLDD161 pKa = 4.01DD162 pKa = 4.22VLKK165 pKa = 10.78LAAFLEE171 pKa = 4.81SS172 pKa = 3.42

Molecular weight: 18.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5BLP3|A0A2H5BLP3_9CAUD HNH endonuclease OS=Streptomyces phage Attoomi OX=2059881 GN=SEA_ATTOOMI_53 PE=4 SV=1
MM1 pKa = 7.46AWYY4 pKa = 9.81GSDD7 pKa = 3.1RR8 pKa = 11.84RR9 pKa = 11.84EE10 pKa = 3.73RR11 pKa = 11.84LPGNWRR17 pKa = 11.84TLRR20 pKa = 11.84AKK22 pKa = 9.65VLKK25 pKa = 10.01RR26 pKa = 11.84DD27 pKa = 3.34GYY29 pKa = 8.95MCVAVLRR36 pKa = 11.84DD37 pKa = 3.24TGLRR41 pKa = 11.84CTAMATDD48 pKa = 3.79VDD50 pKa = 4.93HH51 pKa = 7.26IVPGDD56 pKa = 3.68DD57 pKa = 3.71HH58 pKa = 7.83SLSNLQSLCTWHH70 pKa = 7.13HH71 pKa = 5.35ARR73 pKa = 11.84KK74 pKa = 7.34TAAEE78 pKa = 4.12GAAARR83 pKa = 11.84GRR85 pKa = 11.84RR86 pKa = 11.84VPRR89 pKa = 11.84KK90 pKa = 9.62RR91 pKa = 11.84PADD94 pKa = 3.78RR95 pKa = 11.84HH96 pKa = 5.98PGDD99 pKa = 3.63LAA101 pKa = 5.54

Molecular weight: 11.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12401

43

1141

234.0

25.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.451 ± 0.567

0.605 ± 0.109

6.37 ± 0.41

6.193 ± 0.456

2.379 ± 0.203

8.709 ± 0.335

1.653 ± 0.188

2.984 ± 0.325

3.637 ± 0.382

8.701 ± 0.483

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.105 ± 0.112

2.056 ± 0.13

6.249 ± 0.425

3.008 ± 0.185

7.951 ± 0.491

4.693 ± 0.314

6.435 ± 0.417

8.903 ± 0.381

1.742 ± 0.163

2.177 ± 0.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski