Rhodosalinus sediminis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodosalinus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2966 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3D9BXE9|A0A3D9BXE9_9RHOB Type III glutamate--ammonia ligase OS=Rhodosalinus sediminis OX=1940533 GN=glnT PE=3 SV=1
MM1 pKa = 7.9EE2 pKa = 5.19DD3 pKa = 3.02TMKK6 pKa = 11.2ALLSTAGALALIATSAAANPPTGTPAAFNNTQGPNGSAKK45 pKa = 9.65TVTLFAEE52 pKa = 4.31VFDD55 pKa = 4.83YY56 pKa = 10.94VAVWGEE62 pKa = 3.73EE63 pKa = 3.77DD64 pKa = 3.81SAVLNVADD72 pKa = 4.11VTTDD76 pKa = 3.12SNTSGNNNDD85 pKa = 3.68TNDD88 pKa = 3.02ARR90 pKa = 11.84TDD92 pKa = 3.32KK93 pKa = 11.37ALFTVTSNVTHH104 pKa = 7.73DD105 pKa = 3.72ITLEE109 pKa = 3.78WEE111 pKa = 3.86TWGDD115 pKa = 3.73VNLSNPTNDD124 pKa = 3.1FDD126 pKa = 4.2QANYY130 pKa = 9.53YY131 pKa = 10.63NSAAEE136 pKa = 4.03CSIGGTIKK144 pKa = 10.71LDD146 pKa = 3.75PNPAPEE152 pKa = 5.0FYY154 pKa = 10.96NDD156 pKa = 4.33AISGGGTGTMTHH168 pKa = 6.6EE169 pKa = 4.68DD170 pKa = 3.72AAPTWAHH177 pKa = 6.53EE178 pKa = 4.08YY179 pKa = 11.06GIGTEE184 pKa = 4.02ASPVHH189 pKa = 5.88TNCPGDD195 pKa = 3.19IAAPGTYY202 pKa = 10.23SLDD205 pKa = 3.15VDD207 pKa = 3.66ITVSKK212 pKa = 11.04AGAA215 pKa = 3.33

Molecular weight: 22.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3D9BNG2|A0A3D9BNG2_9RHOB ABC transporter ATP-binding protein OS=Rhodosalinus sediminis OX=1940533 GN=DRV84_12765 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.44AGRR28 pKa = 11.84KK29 pKa = 8.46VLNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2966

0

2966

913301

29

2079

307.9

33.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.064 ± 0.089

0.782 ± 0.012

5.83 ± 0.046

6.709 ± 0.044

3.328 ± 0.027

9.127 ± 0.042

1.975 ± 0.022

4.092 ± 0.031

2.154 ± 0.037

10.157 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.479 ± 0.022

1.824 ± 0.025

5.573 ± 0.034

2.573 ± 0.025

8.268 ± 0.059

4.001 ± 0.028

5.188 ± 0.041

7.484 ± 0.035

1.409 ± 0.018

1.982 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski