Escherichia phage BZ13 (Bacteriophage BZ13)

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Allassoviricetes; Levivirales; Leviviridae; Levivirus

Average proteome isoelectric point is 8.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C8YJG9|C8YJG9_BPBZ1 RNA replicase beta chain OS=Escherichia phage BZ13 OX=329853 PE=4 SV=1
MM1 pKa = 7.37FRR3 pKa = 11.84FTEE6 pKa = 4.06IEE8 pKa = 3.83KK9 pKa = 9.03TLCMDD14 pKa = 4.01RR15 pKa = 11.84TRR17 pKa = 11.84DD18 pKa = 3.47CAVRR22 pKa = 11.84FHH24 pKa = 7.27VYY26 pKa = 9.84LQSLDD31 pKa = 3.7MGSSDD36 pKa = 3.38PHH38 pKa = 8.16SPDD41 pKa = 3.31FDD43 pKa = 3.55GLAYY47 pKa = 10.7LRR49 pKa = 11.84DD50 pKa = 3.68EE51 pKa = 5.36CLTKK55 pKa = 10.6HH56 pKa = 6.26PSLGNSNSDD65 pKa = 3.19ARR67 pKa = 11.84RR68 pKa = 11.84KK69 pKa = 9.05EE70 pKa = 3.55LAYY73 pKa = 10.51AKK75 pKa = 10.79LMDD78 pKa = 4.09SDD80 pKa = 4.22QRR82 pKa = 11.84CKK84 pKa = 10.15IQNSNGYY91 pKa = 9.18DD92 pKa = 3.48YY93 pKa = 11.88SHH95 pKa = 7.24IEE97 pKa = 3.94SSVLSGILKK106 pKa = 8.38TAQALVANLLTGFEE120 pKa = 4.52SHH122 pKa = 7.27FLNDD126 pKa = 3.53CSFSNGASQGFKK138 pKa = 10.78LRR140 pKa = 11.84DD141 pKa = 3.12AAPFKK146 pKa = 10.75KK147 pKa = 9.93IAGQATVTAPAYY159 pKa = 9.54NIAVAAVKK167 pKa = 9.25TCAPWYY173 pKa = 10.42AYY175 pKa = 8.05MQEE178 pKa = 4.29TYY180 pKa = 11.15GDD182 pKa = 3.35EE183 pKa = 4.32TRR185 pKa = 11.84WFRR188 pKa = 11.84RR189 pKa = 11.84VYY191 pKa = 10.78GNGLFSVPKK200 pKa = 9.87NNKK203 pKa = 8.33IDD205 pKa = 3.54RR206 pKa = 11.84AACKK210 pKa = 10.1EE211 pKa = 3.91PDD213 pKa = 3.19MNMYY217 pKa = 9.01LQKK220 pKa = 10.87GAGSFIRR227 pKa = 11.84KK228 pKa = 9.06RR229 pKa = 11.84LRR231 pKa = 11.84SVGIDD236 pKa = 4.97LNDD239 pKa = 3.29QTCNQEE245 pKa = 3.95LARR248 pKa = 11.84LGSIDD253 pKa = 4.18GSLATIDD260 pKa = 4.48LSSASDD266 pKa = 3.65SVSDD270 pKa = 3.73RR271 pKa = 11.84LVWDD275 pKa = 4.22LLPPHH280 pKa = 6.96VYY282 pKa = 10.32SYY284 pKa = 10.95LARR287 pKa = 11.84IRR289 pKa = 11.84SSFTMIDD296 pKa = 3.2GRR298 pKa = 11.84LHH300 pKa = 5.44KK301 pKa = 9.96WGLFSTMGNGFTFEE315 pKa = 4.45LEE317 pKa = 4.66SMIFWALSKK326 pKa = 10.98SVMLSMGVTGSLGIYY341 pKa = 9.83GDD343 pKa = 4.61DD344 pKa = 3.23IIVPVEE350 pKa = 3.98CAPTLLKK357 pKa = 10.81VLSAVNFLPNKK368 pKa = 9.88KK369 pKa = 8.54KK370 pKa = 9.16TFTTGYY376 pKa = 9.79FRR378 pKa = 11.84EE379 pKa = 4.32SCGAHH384 pKa = 5.89FFKK387 pKa = 10.9GADD390 pKa = 3.36MKK392 pKa = 10.8PFYY395 pKa = 10.15CKK397 pKa = 10.27RR398 pKa = 11.84PMEE401 pKa = 4.28TLPDD405 pKa = 3.68VMLLCNRR412 pKa = 11.84IRR414 pKa = 11.84GWQTVGGMSDD424 pKa = 3.34PRR426 pKa = 11.84LFPIWKK432 pKa = 8.89EE433 pKa = 3.85FADD436 pKa = 4.28MIPPKK441 pKa = 10.62FKK443 pKa = 10.78GGCNLDD449 pKa = 3.23RR450 pKa = 11.84DD451 pKa = 4.59TYY453 pKa = 11.03LVSPDD458 pKa = 3.54KK459 pKa = 11.19PGVTLVRR466 pKa = 11.84VAKK469 pKa = 10.23VRR471 pKa = 11.84SGFNHH476 pKa = 6.19SFPYY480 pKa = 9.35GHH482 pKa = 6.64EE483 pKa = 3.96NGRR486 pKa = 11.84YY487 pKa = 6.63VHH489 pKa = 6.72WLHH492 pKa = 6.32MGSGEE497 pKa = 4.1VLEE500 pKa = 5.22TISSARR506 pKa = 11.84FRR508 pKa = 11.84CKK510 pKa = 10.16PNSEE514 pKa = 3.83WRR516 pKa = 11.84TQIPLFPQEE525 pKa = 5.23LEE527 pKa = 4.08ACVLSS532 pKa = 3.99

Molecular weight: 59.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C8YJG7|C8YJG7_BPBZ1 Coat protein OS=Escherichia phage BZ13 OX=329853 PE=4 SV=1
MM1 pKa = 7.25FPKK4 pKa = 10.75SNIDD8 pKa = 3.14RR9 pKa = 11.84NYY11 pKa = 9.93KK12 pKa = 8.32VTLISYY18 pKa = 9.08DD19 pKa = 3.53EE20 pKa = 4.29KK21 pKa = 11.47GKK23 pKa = 10.6LVSDD27 pKa = 4.32DD28 pKa = 4.13SFEE31 pKa = 4.16QVEE34 pKa = 4.6NYY36 pKa = 10.25LFQNRR41 pKa = 11.84SNTYY45 pKa = 7.69KK46 pKa = 10.13PGYY49 pKa = 8.49IRR51 pKa = 11.84RR52 pKa = 11.84DD53 pKa = 3.36FRR55 pKa = 11.84RR56 pKa = 11.84PTNFWNGFRR65 pKa = 11.84YY66 pKa = 9.82FNQPVGTFTRR76 pKa = 11.84KK77 pKa = 10.07LSDD80 pKa = 3.08GGRR83 pKa = 11.84QVADD87 pKa = 3.47YY88 pKa = 11.18GIVNPNKK95 pKa = 8.88FTGNSQHH102 pKa = 6.86LGDD105 pKa = 4.79NMVIYY110 pKa = 9.42PGPFSINIDD119 pKa = 2.87HH120 pKa = 6.84RR121 pKa = 11.84ASVEE125 pKa = 4.02VLNKK129 pKa = 10.41LSQSNLNIGVAIAEE143 pKa = 4.38AKK145 pKa = 7.55MTASLLAKK153 pKa = 10.16QSIALIRR160 pKa = 11.84AYY162 pKa = 9.19TAAKK166 pKa = 8.36RR167 pKa = 11.84GKK169 pKa = 7.83WRR171 pKa = 11.84EE172 pKa = 3.81VLSQLLISEE181 pKa = 4.33HH182 pKa = 6.69RR183 pKa = 11.84FRR185 pKa = 11.84APVKK189 pKa = 10.38DD190 pKa = 4.07LGGRR194 pKa = 11.84WLEE197 pKa = 4.16LQYY200 pKa = 11.47GWLPLMSDD208 pKa = 3.52MKK210 pKa = 10.8AAYY213 pKa = 9.68DD214 pKa = 4.5LLTQTKK220 pKa = 9.72LPAFMPLRR228 pKa = 11.84VTRR231 pKa = 11.84TVGGTHH237 pKa = 6.26NYY239 pKa = 9.16KK240 pKa = 9.95VRR242 pKa = 11.84NVEE245 pKa = 4.16SAGDD249 pKa = 3.35TWSYY253 pKa = 11.33SDD255 pKa = 4.03RR256 pKa = 11.84LSVNYY261 pKa = 9.8RR262 pKa = 11.84IWYY265 pKa = 7.99FISDD269 pKa = 4.2PRR271 pKa = 11.84LAWASSLGLLNPLEE285 pKa = 4.69IYY287 pKa = 9.32WEE289 pKa = 4.0KK290 pKa = 10.17TPWSFVVDD298 pKa = 3.47WFLPVGNLIEE308 pKa = 5.13AMSNPLGLDD317 pKa = 3.5IISGTKK323 pKa = 7.87TWQLEE328 pKa = 4.42SKK330 pKa = 10.88LNATLTASGWSGTAKK345 pKa = 10.38LSAYY349 pKa = 9.86AKK351 pKa = 10.31AYY353 pKa = 10.1DD354 pKa = 3.42RR355 pKa = 11.84STFYY359 pKa = 11.1SFPTPMPYY367 pKa = 10.25VKK369 pKa = 10.55SPLSGLHH376 pKa = 6.17LANALALINQRR387 pKa = 11.84LKK389 pKa = 10.91RR390 pKa = 3.85

Molecular weight: 44.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1115

63

532

278.8

31.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.444 ± 0.91

1.435 ± 0.881

5.291 ± 0.606

3.587 ± 0.434

4.843 ± 0.558

6.726 ± 0.548

1.704 ± 0.419

4.933 ± 0.841

5.74 ± 0.106

10.045 ± 0.999

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.691 ± 0.516

5.022 ± 0.714

4.664 ± 0.52

2.691 ± 0.192

5.83 ± 0.68

9.148 ± 0.534

5.471 ± 0.325

6.368 ± 1.434

2.332 ± 0.496

4.036 ± 0.521
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski