Prune dwarf virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Bromoviridae; Ilarvirus

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q91NQ3|Q91NQ3_9BROM RNA-directed RNA polymerase 2a OS=Prune dwarf virus OX=33760 PE=4 SV=1
MM1 pKa = 8.28DD2 pKa = 4.95SLLEE6 pKa = 3.95FLLPSHH12 pKa = 7.34DD13 pKa = 3.63EE14 pKa = 4.1DD15 pKa = 6.7AIEE18 pKa = 5.0LVDD21 pKa = 3.89TNSVKK26 pKa = 10.52PSFSLSDD33 pKa = 3.28SSGDD37 pKa = 3.14VSPIYY42 pKa = 10.33RR43 pKa = 11.84SLCKK47 pKa = 10.38FFGFLMIRR55 pKa = 11.84DD56 pKa = 3.91TVVRR60 pKa = 11.84PDD62 pKa = 3.16KK63 pKa = 10.48FTGEE67 pKa = 4.84FITLPFVIDD76 pKa = 3.83RR77 pKa = 11.84LRR79 pKa = 11.84MTFDD83 pKa = 4.83LEE85 pKa = 3.95DD86 pKa = 4.88DD87 pKa = 3.98YY88 pKa = 12.05AFPVDD93 pKa = 3.74DD94 pKa = 4.4CSYY97 pKa = 11.5DD98 pKa = 4.4LEE100 pKa = 4.49LTDD103 pKa = 4.71AQLDD107 pKa = 4.02YY108 pKa = 11.85AEE110 pKa = 5.1VLQQQKK116 pKa = 11.16DD117 pKa = 3.88FFSEE121 pKa = 4.09SLGKK125 pKa = 10.59VVVDD129 pKa = 3.62YY130 pKa = 11.29DD131 pKa = 4.14FSLDD135 pKa = 4.34SEE137 pKa = 4.71DD138 pKa = 4.42PSIKK142 pKa = 10.39DD143 pKa = 3.27VVKK146 pKa = 10.69IPDD149 pKa = 4.41EE150 pKa = 4.07ISEE153 pKa = 4.79DD154 pKa = 3.92FPQEE158 pKa = 3.89NVPAEE163 pKa = 4.1IVADD167 pKa = 3.91VPQIVSDD174 pKa = 3.88DD175 pKa = 3.79VEE177 pKa = 4.4EE178 pKa = 4.0QVVAGEE184 pKa = 5.05LIPSCEE190 pKa = 3.98TVNEE194 pKa = 4.24NVCVEE199 pKa = 4.45VKK201 pKa = 10.59FPTHH205 pKa = 6.86LAPKK209 pKa = 9.98FLATHH214 pKa = 6.71LPDD217 pKa = 3.27VDD219 pKa = 4.39RR220 pKa = 11.84VILVDD225 pKa = 3.76GDD227 pKa = 4.1TVDD230 pKa = 5.55GFDD233 pKa = 5.0KK234 pKa = 11.09LISRR238 pKa = 11.84NDD240 pKa = 2.86WFFLKK245 pKa = 10.5KK246 pKa = 10.3LLPNIPLPSDD256 pKa = 3.51GDD258 pKa = 3.89VLLSGIKK265 pKa = 8.34PTSPGVLQFALDD277 pKa = 3.69EE278 pKa = 4.47LFPYY282 pKa = 10.28HH283 pKa = 6.73NLVDD287 pKa = 3.99DD288 pKa = 4.58RR289 pKa = 11.84FFQEE293 pKa = 5.27LVEE296 pKa = 4.7CNDD299 pKa = 3.13IALEE303 pKa = 4.58LDD305 pKa = 3.8DD306 pKa = 5.99CSFDD310 pKa = 3.71LSHH313 pKa = 6.66FVSWDD318 pKa = 3.14KK319 pKa = 11.42GKK321 pKa = 11.01SGVDD325 pKa = 3.33STLMTGQTSNRR336 pKa = 11.84QSTFRR341 pKa = 11.84EE342 pKa = 3.88VALSVKK348 pKa = 9.06KK349 pKa = 10.68RR350 pKa = 11.84NMNVPILNTVLNVEE364 pKa = 4.36DD365 pKa = 4.03VSNKK369 pKa = 8.55IVNRR373 pKa = 11.84FFEE376 pKa = 4.53TVVDD380 pKa = 4.07INLLTTLPDD389 pKa = 3.63VISIGEE395 pKa = 3.9VNYY398 pKa = 10.44FSDD401 pKa = 4.0YY402 pKa = 11.43LKK404 pKa = 11.18GKK406 pKa = 9.97AINDD410 pKa = 3.53DD411 pKa = 3.92EE412 pKa = 6.18LYY414 pKa = 10.87VDD416 pKa = 5.22PICLVSMDD424 pKa = 4.85KK425 pKa = 10.07YY426 pKa = 10.41RR427 pKa = 11.84HH428 pKa = 5.64MIKK431 pKa = 10.09SQLKK435 pKa = 8.41PVEE438 pKa = 4.72DD439 pKa = 3.54NSLMFEE445 pKa = 4.3RR446 pKa = 11.84PLAATITFHH455 pKa = 7.97DD456 pKa = 4.18KK457 pKa = 10.93GKK459 pKa = 10.93VMSTSPIFLAAATRR473 pKa = 11.84LFACLNEE480 pKa = 4.85KK481 pKa = 10.33ISIPSGKK488 pKa = 9.3YY489 pKa = 8.95HH490 pKa = 6.71QLFSLDD496 pKa = 3.33AEE498 pKa = 4.52RR499 pKa = 11.84FDD501 pKa = 5.45SVRR504 pKa = 11.84FWKK507 pKa = 10.54EE508 pKa = 3.1VDD510 pKa = 3.75FSKK513 pKa = 10.67FDD515 pKa = 3.3KK516 pKa = 10.83SQQEE520 pKa = 4.0LHH522 pKa = 6.95HH523 pKa = 6.46EE524 pKa = 4.12IQRR527 pKa = 11.84KK528 pKa = 8.29IFLRR532 pKa = 11.84LGVPQEE538 pKa = 4.65FVNTWFTSHH547 pKa = 6.31CRR549 pKa = 11.84SHH551 pKa = 8.38ISDD554 pKa = 3.32ASGLRR559 pKa = 11.84FSVNYY564 pKa = 8.2QRR566 pKa = 11.84RR567 pKa = 11.84AGDD570 pKa = 3.01ACTYY574 pKa = 10.2LGNTIVTLAALCYY587 pKa = 10.4VYY589 pKa = 10.82DD590 pKa = 3.94LRR592 pKa = 11.84SSNVAMVVASGDD604 pKa = 3.49DD605 pKa = 3.69SLIGSYY611 pKa = 10.72SEE613 pKa = 4.19LDD615 pKa = 3.0RR616 pKa = 11.84SFEE619 pKa = 4.32HH620 pKa = 7.2LFSTLFNFEE629 pKa = 4.2AKK631 pKa = 10.1FPHH634 pKa = 5.93NQPFICSKK642 pKa = 10.66FLLTMPTKK650 pKa = 10.73GGGKK654 pKa = 9.22QVVAVPNPAKK664 pKa = 10.68LLIKK668 pKa = 10.57LGVKK672 pKa = 10.41SMTVDD677 pKa = 4.69KK678 pKa = 10.9FDD680 pKa = 5.55DD681 pKa = 3.94WFQSWLDD688 pKa = 3.97LIHH691 pKa = 6.63YY692 pKa = 9.88FDD694 pKa = 5.6DD695 pKa = 4.5YY696 pKa = 11.83YY697 pKa = 11.18LIEE700 pKa = 4.63VVCSMTSYY708 pKa = 10.81RR709 pKa = 11.84YY710 pKa = 9.59IRR712 pKa = 11.84RR713 pKa = 11.84PSQFLVSAISSLKK726 pKa = 10.88SLFANKK732 pKa = 9.63KK733 pKa = 7.62KK734 pKa = 10.96CKK736 pKa = 10.24DD737 pKa = 3.38FLFPGLRR744 pKa = 11.84DD745 pKa = 3.79KK746 pKa = 11.67NPDD749 pKa = 2.76LSMRR753 pKa = 11.84LDD755 pKa = 3.65TPIKK759 pKa = 10.41IRR761 pKa = 11.84DD762 pKa = 3.65VTSKK766 pKa = 9.59RR767 pKa = 11.84TRR769 pKa = 11.84GKK771 pKa = 10.83KK772 pKa = 9.36KK773 pKa = 9.52MKK775 pKa = 9.9EE776 pKa = 3.82NSHH779 pKa = 5.55FQIDD783 pKa = 4.18GKK785 pKa = 11.4VGGG788 pKa = 4.24

Molecular weight: 89.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q91NQ3|Q91NQ3_9BROM RNA-directed RNA polymerase 2a OS=Prune dwarf virus OX=33760 PE=4 SV=1
MM1 pKa = 7.37SGKK4 pKa = 10.02AIKK7 pKa = 10.06SGKK10 pKa = 7.27PTTRR14 pKa = 11.84SQSFALARR22 pKa = 11.84KK23 pKa = 9.26NNNTTPPAGFVKK35 pKa = 10.47KK36 pKa = 9.7QFPSGSSKK44 pKa = 10.92SISEE48 pKa = 4.12WMLHH52 pKa = 5.59GPNVPVKK59 pKa = 9.8SFSGMISRR67 pKa = 11.84TEE69 pKa = 3.77NLTVNSTASGVYY81 pKa = 7.41YY82 pKa = 9.7TMKK85 pKa = 10.08VRR87 pKa = 11.84EE88 pKa = 4.32LFKK91 pKa = 11.24DD92 pKa = 3.87FAVDD96 pKa = 3.3TKK98 pKa = 11.17VYY100 pKa = 10.39GIVFRR105 pKa = 11.84YY106 pKa = 9.74CLDD109 pKa = 3.27VSNGVYY115 pKa = 10.84GLIKK119 pKa = 10.62GFDD122 pKa = 3.58VNAPVAPNPLQRR134 pKa = 11.84RR135 pKa = 11.84KK136 pKa = 8.55FTAKK140 pKa = 9.56QASGVQILAPTGMTVGDD157 pKa = 4.48IPDD160 pKa = 4.59DD161 pKa = 3.27LWFVIKK167 pKa = 10.42YY168 pKa = 10.8DD169 pKa = 3.69NAFQPNVPVWFCTQYY184 pKa = 10.72LQHH187 pKa = 6.99SMPKK191 pKa = 9.55RR192 pKa = 11.84VEE194 pKa = 4.13VPDD197 pKa = 3.55SVLYY201 pKa = 10.74AEE203 pKa = 5.5RR204 pKa = 11.84DD205 pKa = 3.63TALMDD210 pKa = 3.94AMDD213 pKa = 5.32KK214 pKa = 10.57IVSGG218 pKa = 3.91

Molecular weight: 24.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2354

218

1055

588.5

66.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.202 ± 0.894

1.997 ± 0.416

7.562 ± 0.917

5.353 ± 0.428

5.183 ± 0.919

4.97 ± 0.348

2.421 ± 0.376

5.65 ± 0.314

7.604 ± 0.581

8.794 ± 0.617

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.251 ± 0.202

3.526 ± 0.273

4.291 ± 0.538

2.889 ± 0.175

4.418 ± 0.428

8.709 ± 0.468

5.692 ± 0.495

8.156 ± 0.452

0.892 ± 0.065

3.441 ± 0.473
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski