Pelagibacter phage HTVC031P

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Pelagivirus; unclassified Pelagivirus

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y1NU04|A0A4Y1NU04_9CAUD Uncharacterized protein OS=Pelagibacter phage HTVC031P OX=2283017 GN=P031_gp36 PE=4 SV=1
MM1 pKa = 7.47ANSFVRR7 pKa = 11.84YY8 pKa = 7.28TGNGTTTTYY17 pKa = 10.69AIPFSYY23 pKa = 10.37RR24 pKa = 11.84SAEE27 pKa = 4.12DD28 pKa = 3.34LSTTVAGVNVTAYY41 pKa = 9.24TLDD44 pKa = 3.52AAGTNLTFTTAPANNAAIEE63 pKa = 4.04IRR65 pKa = 11.84RR66 pKa = 11.84TTSQNTKK73 pKa = 10.27LVDD76 pKa = 3.75YY77 pKa = 10.91VSGSVLTEE85 pKa = 3.67NDD87 pKa = 3.94LDD89 pKa = 4.06TDD91 pKa = 3.89SDD93 pKa = 3.77QAFYY97 pKa = 10.38MSQEE101 pKa = 4.76AIDD104 pKa = 3.77KK105 pKa = 10.49AGDD108 pKa = 3.78VISLDD113 pKa = 3.33NVDD116 pKa = 5.31FNWDD120 pKa = 3.32IQNKK124 pKa = 8.13RR125 pKa = 11.84LKK127 pKa = 10.59NVADD131 pKa = 3.99PVDD134 pKa = 3.52NTDD137 pKa = 3.33AVNKK141 pKa = 9.89QFISTNIPNITTVAGIASDD160 pKa = 3.7VTTVANNNANITAVAADD177 pKa = 3.65ATDD180 pKa = 3.18IGTVATNISSVNTVATNINDD200 pKa = 4.03VIAVANDD207 pKa = 3.18LAEE210 pKa = 4.27AVSEE214 pKa = 4.53VEE216 pKa = 4.57TVANDD221 pKa = 3.58LNEE224 pKa = 4.27ATSEE228 pKa = 3.92IDD230 pKa = 3.42TVATNITNVNTVGTNIANVNTVAGVNANVTTVAGISADD268 pKa = 3.56VTGVAGISTAVTNVNSNSTNINAVNANSANINTVAGISSDD308 pKa = 3.61VTSVANISSDD318 pKa = 3.3VAAVEE323 pKa = 4.67NIASNVTTVAGISSDD338 pKa = 3.67VTSVAGISSNVTTVASNNANVSAVGGAITNVNNVGNSIASVNTVATNLASVNSFANTYY396 pKa = 10.15LGASATAPTQDD407 pKa = 4.04PDD409 pKa = 4.31GSSLDD414 pKa = 4.3LGDD417 pKa = 5.38LYY419 pKa = 11.35FDD421 pKa = 3.82TASNTMKK428 pKa = 10.62VYY430 pKa = 10.66GSSGWTAAGSSVNGTASRR448 pKa = 11.84FKK450 pKa = 9.8YY451 pKa = 8.75TATSGQTTFTGTDD464 pKa = 3.49DD465 pKa = 3.89NSATLAYY472 pKa = 10.09DD473 pKa = 3.52AGFLDD478 pKa = 4.4VYY480 pKa = 11.11LNGIRR485 pKa = 11.84LVNGTDD491 pKa = 4.05FTASTGNSIVLTTGANLNDD510 pKa = 3.44ILEE513 pKa = 4.34IVAFGTFALANFSITDD529 pKa = 3.59ATDD532 pKa = 3.24VPPLGSAGQALVVNSGGTALEE553 pKa = 4.1FSNASSAEE561 pKa = 3.88VYY563 pKa = 10.55GFHH566 pKa = 7.42KK567 pKa = 10.63DD568 pKa = 3.38SNGDD572 pKa = 4.12LIVTTTNQGVDD583 pKa = 3.74NISSATYY590 pKa = 8.63ATFDD594 pKa = 3.88DD595 pKa = 4.41VLFSASGFTFSLNNGEE611 pKa = 4.99LIATII616 pKa = 4.25

Molecular weight: 62.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y1NTZ6|A0A4Y1NTZ6_9CAUD Lectin domain containing protein OS=Pelagibacter phage HTVC031P OX=2283017 GN=P031_gp42 PE=4 SV=1
MM1 pKa = 7.28PAKK4 pKa = 10.22RR5 pKa = 11.84YY6 pKa = 8.18QSPSGGLNAAGRR18 pKa = 11.84RR19 pKa = 11.84YY20 pKa = 9.67FKK22 pKa = 10.8RR23 pKa = 11.84KK24 pKa = 7.88TGANLKK30 pKa = 10.69APVTGKK36 pKa = 10.18VKK38 pKa = 10.54RR39 pKa = 11.84GSKK42 pKa = 8.47AAKK45 pKa = 9.57RR46 pKa = 11.84RR47 pKa = 11.84ASFCARR53 pKa = 11.84MSGVKK58 pKa = 10.22GAMKK62 pKa = 10.17KK63 pKa = 10.48PNGQPTRR70 pKa = 11.84KK71 pKa = 9.87ALALRR76 pKa = 11.84KK77 pKa = 8.38WKK79 pKa = 10.43CRR81 pKa = 3.49

Molecular weight: 8.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12937

40

1345

244.1

27.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.668 ± 0.421

0.819 ± 0.15

6.068 ± 0.212

6.292 ± 0.429

3.795 ± 0.149

6.423 ± 0.373

1.639 ± 0.187

6.23 ± 0.184

8.333 ± 0.692

7.969 ± 0.336

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.373 ± 0.189

6.47 ± 0.427

3.115 ± 0.202

4.019 ± 0.265

3.594 ± 0.249

6.671 ± 0.445

7.49 ± 0.552

6.137 ± 0.389

1.19 ± 0.114

3.703 ± 0.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski