Nocardioides sp. HDW12B

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3834 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G7XQD2|A0A6G7XQD2_9ACTN 5-oxoprolinase subunit PxpB OS=Nocardioides sp. HDW12B OX=2714939 GN=pxpB PE=4 SV=1
MM1 pKa = 7.75RR2 pKa = 11.84RR3 pKa = 11.84PKK5 pKa = 10.53SLLFAIPLTLALALTACSAEE25 pKa = 4.21SDD27 pKa = 3.82SSGGDD32 pKa = 3.21SSSEE36 pKa = 3.94SSSSSEE42 pKa = 3.95GGEE45 pKa = 4.28SAGTAGGTFSVSSSEE60 pKa = 4.29PEE62 pKa = 3.75AFAPTSNCYY71 pKa = 10.55SSDD74 pKa = 3.19CSQIINAVFTGLLKK88 pKa = 10.4VDD90 pKa = 4.63PEE92 pKa = 4.34TSEE95 pKa = 4.49PIYY98 pKa = 10.58TGAEE102 pKa = 4.02SFEE105 pKa = 4.62SEE107 pKa = 5.46DD108 pKa = 3.67GQNWTIKK115 pKa = 10.59LKK117 pKa = 11.04DD118 pKa = 3.23GFTFHH123 pKa = 7.05NGEE126 pKa = 4.08PVDD129 pKa = 3.62AEE131 pKa = 4.49SYY133 pKa = 9.16VRR135 pKa = 11.84AWNYY139 pKa = 9.38AANGANATQLGFFFSKK155 pKa = 11.01VEE157 pKa = 4.51GYY159 pKa = 11.04DD160 pKa = 3.58DD161 pKa = 4.5LQGEE165 pKa = 4.28NPKK168 pKa = 10.54AEE170 pKa = 4.28EE171 pKa = 4.03MSGLTVVDD179 pKa = 4.36DD180 pKa = 3.99LTFEE184 pKa = 4.39VALSEE189 pKa = 4.36PFSQFPLTMSYY200 pKa = 9.66TPAAAPMAQEE210 pKa = 4.52CMDD213 pKa = 5.56DD214 pKa = 3.68IKK216 pKa = 11.4ACNEE220 pKa = 3.73QPIGNGPYY228 pKa = 10.03QFAEE232 pKa = 4.22KK233 pKa = 9.59WNHH236 pKa = 4.8NQSITLEE243 pKa = 4.18KK244 pKa = 10.08YY245 pKa = 10.38ADD247 pKa = 3.73YY248 pKa = 11.11SDD250 pKa = 4.85EE251 pKa = 4.21EE252 pKa = 4.56TAGLADD258 pKa = 3.38GMEE261 pKa = 4.2FKK263 pKa = 10.38IYY265 pKa = 10.87ADD267 pKa = 3.79LSTAFRR273 pKa = 11.84DD274 pKa = 4.27FQGGNLDD281 pKa = 3.82IVIPDD286 pKa = 4.22PSQIPQARR294 pKa = 11.84AQYY297 pKa = 10.76ADD299 pKa = 3.52SLLEE303 pKa = 3.87VDD305 pKa = 4.58SGSFAYY311 pKa = 10.31FGFPFYY317 pKa = 10.94EE318 pKa = 4.58PDD320 pKa = 3.37FQKK323 pKa = 11.16PEE325 pKa = 3.53IRR327 pKa = 11.84QALSLAIDD335 pKa = 3.66RR336 pKa = 11.84QSIIDD341 pKa = 3.69NVLQGRR347 pKa = 11.84FIPATDD353 pKa = 3.81VISPFVPGSRR363 pKa = 11.84DD364 pKa = 3.55DD365 pKa = 3.9ACEE368 pKa = 3.77FCVYY372 pKa = 10.8DD373 pKa = 3.85PDD375 pKa = 4.01QAKK378 pKa = 10.01EE379 pKa = 3.92LWDD382 pKa = 3.68GAGGVEE388 pKa = 4.38GNEE391 pKa = 3.39ISIWFNNDD399 pKa = 2.37GGHH402 pKa = 5.05EE403 pKa = 4.04QWVKK407 pKa = 11.29AMAQGWQQDD416 pKa = 3.66LGVDD420 pKa = 4.32FTFKK424 pKa = 10.56SQPFTPYY431 pKa = 10.55LKK433 pKa = 10.84ALGTQSEE440 pKa = 4.41VDD442 pKa = 3.23GPYY445 pKa = 10.62RR446 pKa = 11.84LGWLPDD452 pKa = 3.49YY453 pKa = 9.91PSPQNYY459 pKa = 9.92LDD461 pKa = 3.86PLYY464 pKa = 11.32GEE466 pKa = 5.05GSSNYY471 pKa = 9.41GKK473 pKa = 9.81WGEE476 pKa = 4.11GDD478 pKa = 3.5SAADD482 pKa = 3.36QQEE485 pKa = 3.89FLDD488 pKa = 4.72LVNEE492 pKa = 4.3GDD494 pKa = 3.45AAATVEE500 pKa = 4.31EE501 pKa = 5.54GIPSYY506 pKa = 10.55QAAADD511 pKa = 3.78VVLDD515 pKa = 3.74NMVVIPLWFGQTFYY529 pKa = 11.9VMSEE533 pKa = 3.59NVEE536 pKa = 4.09NVKK539 pKa = 10.62YY540 pKa = 10.98SPTDD544 pKa = 3.17QLLLDD549 pKa = 5.27EE550 pKa = 5.26ISVTSS555 pKa = 3.62

Molecular weight: 60.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G7XKU7|A0A6G7XKU7_9ACTN GNAT family N-acetyltransferase OS=Nocardioides sp. HDW12B OX=2714939 GN=G7072_01835 PE=4 SV=1
MM1 pKa = 7.28GSVVKK6 pKa = 10.49KK7 pKa = 9.45RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.48RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.12TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84LGKK33 pKa = 10.04

Molecular weight: 4.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3834

0

3834

1259540

33

3771

328.5

35.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.926 ± 0.061

0.723 ± 0.01

6.697 ± 0.032

5.996 ± 0.033

2.711 ± 0.021

9.419 ± 0.036

2.166 ± 0.019

2.915 ± 0.033

1.797 ± 0.034

10.304 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.755 ± 0.017

1.634 ± 0.024

5.748 ± 0.028

2.669 ± 0.019

7.956 ± 0.05

5.35 ± 0.029

6.151 ± 0.03

9.735 ± 0.041

1.485 ± 0.015

1.861 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski