Bos indicus (Zebu)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria;

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 30764 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P5DDE2|A0A6P5DDE2_BOSIN keratin-associated protein 4-7-like isoform X1 OS=Bos indicus OX=9915 GN=LOC109573472 PE=4 SV=1
MM1 pKa = 7.76LEE3 pKa = 4.49PPLFLPAGWGCAQALTSPSLTSQGLSFIIFVCYY36 pKa = 9.76VASSASAFLTAPLLEE51 pKa = 4.94FLLALCFLFADD62 pKa = 5.01AMQLNDD68 pKa = 3.76KK69 pKa = 9.27WQGLCWPMMDD79 pKa = 5.28FLRR82 pKa = 11.84CVTAALIYY90 pKa = 9.36FAISITAVAKK100 pKa = 10.97YY101 pKa = 10.15PDD103 pKa = 4.43GASKK107 pKa = 10.66AAGVFGFFATIVFAIDD123 pKa = 4.16FYY125 pKa = 11.6LIFNDD130 pKa = 3.21VAKK133 pKa = 10.37FLKK136 pKa = 10.42QEE138 pKa = 3.78XSAQEE143 pKa = 3.83TTADD147 pKa = 3.54KK148 pKa = 11.41AEE150 pKa = 4.51EE151 pKa = 4.21EE152 pKa = 4.85DD153 pKa = 4.14SDD155 pKa = 4.61SDD157 pKa = 4.08SDD159 pKa = 3.65

Molecular weight: 17.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P5BKQ1|A0A6P5BKQ1_BOSIN oxidoreductase HTATIP2 OS=Bos indicus OX=9915 GN=HTATIP2 PE=4 SV=1
MM1 pKa = 7.23TKK3 pKa = 8.67VTRR6 pKa = 11.84KK7 pKa = 8.51PRR9 pKa = 11.84QSRR12 pKa = 11.84RR13 pKa = 11.84VPMRR17 pKa = 11.84FASRR21 pKa = 11.84MNGKK25 pKa = 9.72KK26 pKa = 8.49KK27 pKa = 8.62TLCQRR32 pKa = 11.84RR33 pKa = 11.84YY34 pKa = 9.31RR35 pKa = 11.84GSVKK39 pKa = 10.12ARR41 pKa = 11.84NMTMRR46 pKa = 11.84VRR48 pKa = 11.84RR49 pKa = 11.84PLQGTLRR56 pKa = 11.84KK57 pKa = 9.72KK58 pKa = 10.11IRR60 pKa = 11.84SHH62 pKa = 5.74ATQSKK67 pKa = 9.32KK68 pKa = 9.61VKK70 pKa = 8.94KK71 pKa = 8.72TRR73 pKa = 11.84TPNCFFRR80 pKa = 11.84SCARR84 pKa = 11.84KK85 pKa = 9.79KK86 pKa = 10.58LNQSRR91 pKa = 11.84KK92 pKa = 7.74RR93 pKa = 11.84SQRR96 pKa = 11.84MRR98 pKa = 11.84RR99 pKa = 11.84NQRR102 pKa = 11.84RR103 pKa = 11.84RR104 pKa = 11.84QNPKK108 pKa = 9.71RR109 pKa = 11.84RR110 pKa = 3.59

Molecular weight: 13.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19672

11092

30764

18860430

11

33566

613.1

68.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.999 ± 0.014

2.142 ± 0.012

4.82 ± 0.01

7.208 ± 0.018

3.521 ± 0.011

6.413 ± 0.018

2.565 ± 0.008

4.227 ± 0.011

5.762 ± 0.018

9.89 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.117 ± 0.006

3.521 ± 0.011

6.421 ± 0.02

4.856 ± 0.013

5.729 ± 0.014

8.548 ± 0.02

5.274 ± 0.011

5.966 ± 0.012

1.158 ± 0.005

2.572 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski