Mycobacterium phage TM4 (Mycobacteriophage TM4)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; Mycobacterium virus TM4

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9ZX40|Q9ZX40_BPMT4 Uncharacterized protein OS=Mycobacterium phage TM4 OX=88870 GN=38 PE=4 SV=1
MM1 pKa = 7.55SDD3 pKa = 3.86EE4 pKa = 4.09VWVLDD9 pKa = 4.65FEE11 pKa = 5.41AEE13 pKa = 4.29GPEE16 pKa = 4.04PGDD19 pKa = 3.38YY20 pKa = 10.64VGYY23 pKa = 10.33QSVHH27 pKa = 4.62RR28 pKa = 11.84TRR30 pKa = 11.84DD31 pKa = 3.27GASNRR36 pKa = 11.84LLEE39 pKa = 4.28RR40 pKa = 11.84LADD43 pKa = 3.58VDD45 pKa = 3.67VDD47 pKa = 4.01VAEE50 pKa = 4.43VEE52 pKa = 4.42ALGGAQADD60 pKa = 3.94DD61 pKa = 3.59GSYY64 pKa = 10.74AGEE67 pKa = 4.18LDD69 pKa = 3.64ADD71 pKa = 4.11GMTISYY77 pKa = 9.56GVHH80 pKa = 6.18RR81 pKa = 11.84MPIEE85 pKa = 4.19DD86 pKa = 3.32

Molecular weight: 9.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9ZX32|Q9ZX32_BPMT4 Uncharacterized protein OS=Mycobacterium phage TM4 OX=88870 GN=46 PE=4 SV=1
MM1 pKa = 7.7SDD3 pKa = 3.94LVIFDD8 pKa = 4.41EE9 pKa = 4.58LQQGEE14 pKa = 4.53EE15 pKa = 4.01PRR17 pKa = 11.84DD18 pKa = 3.46YY19 pKa = 11.49VPAFEE24 pKa = 4.72RR25 pKa = 11.84AVLYY29 pKa = 11.04AMQFKK34 pKa = 10.19PMYY37 pKa = 9.11EE38 pKa = 3.84GTVPHH43 pKa = 6.92RR44 pKa = 11.84VRR46 pKa = 11.84AKK48 pKa = 8.72RR49 pKa = 11.84RR50 pKa = 11.84EE51 pKa = 3.96RR52 pKa = 11.84NRR54 pKa = 11.84VARR57 pKa = 11.84RR58 pKa = 11.84SRR60 pKa = 11.84KK61 pKa = 9.21INRR64 pKa = 11.84KK65 pKa = 9.62AII67 pKa = 3.72

Molecular weight: 8.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

16776

29

1229

182.3

19.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.096 ± 0.471

1.073 ± 0.165

6.748 ± 0.206

5.705 ± 0.373

2.665 ± 0.134

8.703 ± 0.438

2.355 ± 0.199

3.94 ± 0.228

3.636 ± 0.259

8.274 ± 0.202

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.337 ± 0.129

2.706 ± 0.157

5.895 ± 0.249

3.499 ± 0.19

7.111 ± 0.384

4.954 ± 0.271

5.538 ± 0.192

7.558 ± 0.262

2.086 ± 0.112

2.122 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski