Streptococcus phage SpGS-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C9LX51|A0A1C9LX51_9CAUD Uncharacterized protein OS=Streptococcus phage SpGS-1 OX=1897555 PE=4 SV=1
MM1 pKa = 7.62TEE3 pKa = 4.23TIKK6 pKa = 11.18LPDD9 pKa = 3.79YY10 pKa = 11.12YY11 pKa = 11.1EE12 pKa = 4.8PDD14 pKa = 3.33WKK16 pKa = 10.42NARR19 pKa = 11.84YY20 pKa = 9.81GSLEE24 pKa = 3.85EE25 pKa = 4.48LKK27 pKa = 10.89EE28 pKa = 3.74LLLFKK33 pKa = 10.71RR34 pKa = 11.84IVKK37 pKa = 8.85WDD39 pKa = 3.45KK40 pKa = 11.44DD41 pKa = 3.71FLLLEE46 pKa = 5.42DD47 pKa = 3.96GTKK50 pKa = 8.98VTIEE54 pKa = 4.02MSEE57 pKa = 4.16SDD59 pKa = 4.01CCASAGGEE67 pKa = 4.1FQDD70 pKa = 3.79VSLDD74 pKa = 3.64AVITNIEE81 pKa = 3.73IGEE84 pKa = 4.13PEE86 pKa = 5.23EE87 pKa = 5.91IPDD90 pKa = 3.42HH91 pKa = 6.55WGTGYY96 pKa = 10.68KK97 pKa = 10.37NKK99 pKa = 8.63VTIFHH104 pKa = 6.23NQNPVAIANCEE115 pKa = 4.04AEE117 pKa = 4.32HH118 pKa = 6.09NGYY121 pKa = 10.13YY122 pKa = 10.54YY123 pKa = 10.28SVCSLVIGDD132 pKa = 3.1IHH134 pKa = 6.93FPVVNAA140 pKa = 4.65

Molecular weight: 15.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C9LX70|A0A1C9LX70_9CAUD Tail-length tape measure protein OS=Streptococcus phage SpGS-1 OX=1897555 PE=4 SV=1
MM1 pKa = 7.54SIAIKK6 pKa = 10.64VDD8 pKa = 3.5LQKK11 pKa = 11.21AKK13 pKa = 10.59QKK15 pKa = 10.71LSNEE19 pKa = 3.7SMTRR23 pKa = 11.84GKK25 pKa = 10.05IAVASKK31 pKa = 10.45ILLDD35 pKa = 3.62NEE37 pKa = 4.16QYY39 pKa = 10.4IPLRR43 pKa = 11.84GGEE46 pKa = 4.01LRR48 pKa = 11.84ASGRR52 pKa = 11.84IVGQGDD58 pKa = 3.52AVVYY62 pKa = 7.1GTVYY66 pKa = 10.93ARR68 pKa = 11.84AQFYY72 pKa = 9.69GANGIVTFRR81 pKa = 11.84RR82 pKa = 11.84YY83 pKa = 5.87TTPGTGKK90 pKa = 10.24RR91 pKa = 11.84WDD93 pKa = 3.44QVATSKK99 pKa = 10.7HH100 pKa = 5.05AEE102 pKa = 3.36EE103 pKa = 4.01WARR106 pKa = 11.84AFVKK110 pKa = 10.83GMGLL114 pKa = 3.32

Molecular weight: 12.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

11840

53

1152

223.4

25.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.909 ± 0.555

0.498 ± 0.075

6.47 ± 0.292

7.323 ± 0.475

4.231 ± 0.264

6.715 ± 0.363

1.512 ± 0.191

6.909 ± 0.328

8.361 ± 0.359

7.568 ± 0.297

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.28 ± 0.184

5.938 ± 0.286

2.694 ± 0.182

4.299 ± 0.28

4.24 ± 0.224

6.149 ± 0.436

6.503 ± 0.38

6.242 ± 0.21

1.25 ± 0.135

3.91 ± 0.317
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski