Leptospira ryugenii

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Leptospirales; Leptospiraceae; Leptospira

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3659 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P2E5F7|A0A2P2E5F7_9LEPT Methyltransferase OS=Leptospira ryugenii OX=1917863 GN=LPTSP4_36450 PE=3 SV=1
MM1 pKa = 7.01FAEE4 pKa = 5.42LEE6 pKa = 4.42SSCNGTTAFGNAGTGGDD23 pKa = 3.99FNLLQSNTAPAGYY36 pKa = 8.6PYY38 pKa = 11.63YY39 pKa = 9.77MISVDD44 pKa = 3.59TTAKK48 pKa = 10.42DD49 pKa = 3.51VNNVSFPSTFNFSMEE64 pKa = 3.99AKK66 pKa = 10.21

Molecular weight: 7.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P2DXK1|A0A2P2DXK1_9LEPT DedA-like protein OS=Leptospira ryugenii OX=1917863 GN=LPTSP4_08630 PE=3 SV=1
MM1 pKa = 7.29VDD3 pKa = 4.26SRR5 pKa = 11.84FLLDD9 pKa = 4.16GFLDD13 pKa = 3.99LVEE16 pKa = 4.05GLIWGMVRR24 pKa = 11.84SRR26 pKa = 11.84SRR28 pKa = 11.84IRR30 pKa = 11.84RR31 pKa = 11.84SLYY34 pKa = 10.38LINHH38 pKa = 5.51VHH40 pKa = 6.78PAQNVRR46 pKa = 11.84KK47 pKa = 8.93QKK49 pKa = 10.69GMLASIYY56 pKa = 10.57HH57 pKa = 6.16PFGG60 pKa = 4.62

Molecular weight: 7.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3659

0

3659

1185067

23

2707

323.9

36.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.137 ± 0.036

0.773 ± 0.011

4.891 ± 0.025

7.237 ± 0.044

5.541 ± 0.037

6.368 ± 0.037

1.853 ± 0.02

7.484 ± 0.032

7.446 ± 0.042

10.569 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.046 ± 0.017

4.546 ± 0.03

3.937 ± 0.023

3.718 ± 0.022

4.446 ± 0.025

7.569 ± 0.037

4.951 ± 0.029

5.769 ± 0.031

1.13 ± 0.015

3.59 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski