Brachionus plicatilis (Marine rotifer) (Brachionus muelleri)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Spiralia; Gnathifera; Rotifera; Eurotatoria; Monogononta; Pseudotrocha; Ploima; Brachionidae; Brachionus

Average proteome isoelectric point is 7.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52387 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M7PUD6|A0A3M7PUD6_BRAPC Uncharacterized protein OS=Brachionus plicatilis OX=10195 GN=BpHYR1_033835 PE=4 SV=1
MM1 pKa = 7.42LNIRR5 pKa = 11.84IGSAKK10 pKa = 9.51TLQLNCAVEE19 pKa = 4.76LSLCVIFNQDD29 pKa = 2.13VRR31 pKa = 11.84FNYY34 pKa = 10.21DD35 pKa = 3.21LNNVLVKK42 pKa = 10.36SYY44 pKa = 11.02QEE46 pKa = 3.79ILKK49 pKa = 10.71VSAKK53 pKa = 10.06YY54 pKa = 9.61YY55 pKa = 10.72RR56 pKa = 11.84PDD58 pKa = 3.51TVSDD62 pKa = 4.23SIGDD66 pKa = 4.41LYY68 pKa = 11.17QEE70 pKa = 4.1AVLYY74 pKa = 7.56EE75 pKa = 4.27TEE77 pKa = 3.97GDD79 pKa = 4.03GYY81 pKa = 11.15DD82 pKa = 5.11DD83 pKa = 4.63EE84 pKa = 5.43TDD86 pKa = 3.38QSDD89 pKa = 4.01TNSEE93 pKa = 4.09VSDD96 pKa = 4.44DD97 pKa = 3.76EE98 pKa = 4.71SLL100 pKa = 3.65

Molecular weight: 11.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M7PK26|A0A3M7PK26_BRAPC Uncharacterized protein OS=Brachionus plicatilis OX=10195 GN=BpHYR1_034096 PE=4 SV=1
MM1 pKa = 6.69FTKK4 pKa = 10.53PVQSSNRR11 pKa = 11.84FSKK14 pKa = 10.45PVQSSNRR21 pKa = 11.84FSKK24 pKa = 10.32QVPSSNRR31 pKa = 11.84FTKK34 pKa = 10.26PVSSSNRR41 pKa = 11.84FTKK44 pKa = 10.21QVSSSNRR51 pKa = 11.84FSKK54 pKa = 10.37PVPSSNRR61 pKa = 11.84FTKK64 pKa = 10.26PVSSSNRR71 pKa = 11.84FSKK74 pKa = 10.37PVPSSNRR81 pKa = 11.84FTKK84 pKa = 10.21PVPTSNRR91 pKa = 11.84FSKK94 pKa = 10.65PVFLL98 pKa = 5.84

Molecular weight: 11.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52211

176

52387

10963705

8

6181

209.3

24.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.295 ± 0.011

2.052 ± 0.01

5.033 ± 0.009

6.118 ± 0.016

5.834 ± 0.013

3.836 ± 0.014

2.09 ± 0.006

7.62 ± 0.011

8.757 ± 0.017

10.114 ± 0.015

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.17 ± 0.005

7.183 ± 0.015

3.373 ± 0.01

4.06 ± 0.011

4.315 ± 0.01

8.661 ± 0.017

4.888 ± 0.01

5.037 ± 0.01

0.942 ± 0.004

3.552 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski